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The Effect of Silybinin on the Expression of Vascular Endothelial Growth Factor (VEGF) and Integrin-linked kinase (ILK) in the Olfactory Ensheathing Cells under Normal and High Glucose Conditions
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Saeedeh Rashidi1    , Monireh Azizi 2, Naser Abbasi3 , Somaye Heidarizadi4 |
1- MSc of Anatomy, Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
2- Associate Professor of Anatomy, Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran , azizi.moaz@gmail.com
3- Associate Professor of pharmacology, Biotechnology and Medicinal Plants Research Center, Department of Pharmacology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran
4- MSc of Anatomy, Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran |
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Abstract: (962 Views) |
Background and Aim: Diabetes is a metabolic disease with high blood sugar levels. Olfactory ensheathing cells (OECs) are able to express various growth factors and integrin-linked kinase (ILK) plays an important role in regulating various processes of cells. In this study, we investigated the effects of silybinin on the expression of vascular-endothelial growth factor (VEGF) and ILK under normal and high glucose conditions in OECs.
Materials and Methods: In this study, OECs were extracted from the olfactory mucosa of neonatal rats and cultured. Low (1,5M) and high (50,75 µM) concentrations of silybinin were added to the cell culture media under normal and high glucose conditions. At the end of the study, we measured the expression of VEGF and ILK proteins using western blot technique. Data were analyzed by ANOVA test.
Results: Under high glucose conditions, low concentrations of silybinin maintained expression and at high concentrations silybinin caused a dose-dependent decrease in ILK significantly. However, under high glucose conditions, low concentrations of silybinin significantly decreased expression of VEGF but high concentrations of silybinin caused a significant dose- dependent increase in VEGF expression.
Conclusion: High glucose condition increased ILK and VEGF expression in the OECs, and treatment with lower concentrations of silybinin maintained ILK expression and decreased VEGF expression. Therefore, it seems that lower concentrations of silybinin can be effective in protecting cells from cytotoxicity due to diabetes hyperglycemia by stabilizing ILK and reducing VEGF levels. |
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| Keywords: Silybinin, Integrin-link kinase, Growth factors, Olfactory ensheathing cells, High glucose |
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Full-Text [PDF 1350 kb]
(310 Downloads)
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Type of Study: Original Research |
Subject:
Molecular Medicine and Genetics
Received: 2021/06/3 | Accepted: 2021/10/26 | Published: 2023/01/29
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