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Showing 2 results for Origanum
Ali Mohammadi-Gollou, Reza Mohammadzadeh, Mohsen Sagha, Mohammad Mohammadzadeh-Vardin,
Volume 25, Issue 6 (1-2021)
Abstract
Background and Aim: Acute promyelocytic leukemia is a malignancy of myeloid cells that are associated with resistance to apoptosis and differentiation arrest in promyelocytes in the bone marrow. The aim of this study was to investigate the effect of Origanum vulgare aqueous extract on apoptosis induction in an acute promyelocytic leukemia cell line (HL-60).
Materials and Methods: The viability of HL-60 cells under treatment with various doses of Origanum vulgare extract was assessed by using a cell cytotoxic assay. Then, IC50 values were determined after 24, 48, and 72 hours. The expression of Caspase8, Caspase9, Bax, Bcl2, and Nrf2 genes were measured by real-time PCR assay and different stages of apoptosis and necrosis in HL-60 cells were investigated using acridine orange/ethidium bromide double staining. Finally, data were introduced into SPSS software and analyzed by one-way ANOVA.
Results: The results showed that Origanum vulgare extract decreased HL-60 cell survival and Caspase9, Caspase8, Bax, Bcl2, and Nrf2 gene expressions were changed after 72 hours of treatment with 1/5 of IC50 of Origanum vulgare extract. Also, morphological changes in the nucleus indicated apoptosis induction in HL-60 cells.
Conclusion: It seems that the aqueous extract of Origanum vulgare leaves can induce apoptosis in an acute promyelocytic leukemia cell line (HL-60) under experimental conditions.
Sajjad Abbasi, Sohrab Azin, Masoumeh Fani, Malihe Soltani, Seyed Hosein Abtahi-Eivary, Maryam Moghimian,
Volume 27, Issue 2 (5-2022)
Abstract
Background and Aim: The aim of this study was to investigate the effect of Origanum vulgare administration on testicular injury induced by torsion/detorsion .
Materials and Methods: In this experimental study, 64 adult rats were divided into 8 equal groups, as following:1-Sham: the abdominal cavity was opened and then closed. 2.Torsion/detorsion: the rats underwent spermatic cord twisting for 4 hours and then untwisting (30 minutes before untwisting normal saline was injected). 3.Torsion/detorsion+ Origanum vulgare: Similar to group 2, the only difference was injections of Origanum vulgare with different doses (400 mg/kg, 250, 100) which were given to 3 separate groups. 4. Origanum vulgare recipient: without any surgery, injections of Origanum vulgare were given to 3 separate groups with different doses (400 mg/kg, 250, 100). Twenty-four hours after surgery, blood samples were taken to measure oxidative stress factors. Testicular tissue was removed for assessment of histological changes, and the epididymis tissue was used to measure sperm parameters. Data were analyzed by SPSS software.
Results: We found significant decreases in Johnson's score, epithelium height, and seminiferous tubule diameter (data obtained from histological examination), number and morphology of sperm parameters and oxidative stress factors (Malondialdehyde, Superoxide dismutase, Glutathione Peroxidase and, Catalase) in the torsion/detorsion group compared to those in the sham group (P= 0.0001), while they improved in the torsion/detorsion groups receiving Origanum vulgare group compared to those in the torsion/detorsion group, but in most cases, the results were not significant.
Conclusion: It seems that Origanum vulgare can partially reduce the damage caused by testicular torsion/detorsion.
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