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Journal Citation Index

 

Citation Indices from GS

AllSince 2020
Citations102755683
h-index3925
i10-index268141

 

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Central Library of Kurdistan University of Medical Sciences
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Vice-Chancellery for Research and Technology
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:: Search published articles ::
Showing 10 results for Expression

Nasim Ghorbanmehr, Dr Mansoureh Movahedin, Dr Seyed Javad Mola,
Volume 11, Issue 1 (6-2006)
Abstract

ABSTRACT Background and Aim: Successful development and implantation of cryo-preserved embryos depend on suitable culture medium, cytokines, growth factors and genomic expression. Epidermal growth factor and its receptor have important roles on embryonic development and implantation. The purpose of this study was to investigate the influence of EGF on preimplantation development and to detect mRNA for the EGF receptor in vitrified mouse embryo. Materials and Methods: 8 to 16 celled mouse embryos were collected from super-ovulated NMRI mice 56 to 64 h after HCG injection and divided into 4 groups: two control groups were cultured in MEM-α (control 1) and MEM-α+EGF (10 ng/ml) as control 2. The experimental groups were vitrified immediately after collection and then cultured for 96h in MEM-α and M-α+EGF (l0 ng/ml) respectively. Expression of EGFR in hatched and hatching blastocysts was studied too. Following RT, PCR nested primers were designed to optimize the specificity. Results: The results showed that blastocyst formation and hatching rates were significantly lower in vitrified embryos and degeneration rate was significantly higher in these groups. Blastocyst formation, hatching and degeneration rates were not significantly different in control group 1 compared to control group 2 and also in exp. group 1 in comparison with exp. group 2. 96 hours after culture, mRNA expression of EGFR was detected in embryos in the four groups. Conclusion: In conclusion addition of EGF (10 ng/ml) to a complex medium like (MEM-α) doesn’t have any stimulatory effect on embryonic development of vitrified and non-vitrified embryos. Vitrification did not prevent EGFR expression after 96h of culturing, but it seems that addition of EGF to the medium stimulates EGFR expression in mouse blastocysts. Key words: Preimplantation embryo, Epidermal growth factor, Cryopreservation, Gene expression.
Maryam Moradi Nasab, Dr Mohammad Ali Hossein Pour Feizi, Reyhaneh Ravanbakhsh Gavgani, Dr Naser Pouladi, Aeeda Sedaie Bonab, Parvin Azarfam, Dr Vahid Montazeri, Dr Ashraf Fakhrjoo,
Volume 19, Issue 3 (10-2014)
Abstract

Background and Aim: The TP63 gene, as the oldest homologue of TP53, encodes two main N-terminal variants by different promoters: the trans-activating variant, TAp63 with tumor suppressor activity and the amino terminal truncated variant, ΔNp63 with oncogenic activity. As the result of the deletion of exon 4 in each of the variants, two further N-terminal variants have been reported for p63 in the last decade: d4TAp63 and ΔNp73L, but the exact function of these variants have not been determined in normal and tumoral cells. In this study we evaluated the expression pattern of p63 variants and usefulness of d4TAp63 and ΔNp73L as potential diagnostic molecular markers in breast cancer. Materials and Methods: In this study 30 tumoral and 20 non tumoral samples of marginal tissues were studied by use of Semi-quantitative Reverse Transcriptase-nested PCR (RT-nPCR) method. SPSS16 software was used for data analysis. Results: In all cases with expression of TAp63 and ΔNp63 variants, d4TAp63 and ΔNp73L variants were always expressed with their long variants simultaneously. In tumoral and marginal samples ΔNp73L mean expression level was significantly higher than the mean expression level of d4TAP63 (P = 0.009&P=0.008 respectively). ΔNp63 expression levels in tumoral and marginal samples were also higher than those of TAp63, which had a statistically significant difference in tumoral samples (P=0.03), but no significant difference was detected in marginal samples (P = 0.11). Conclusion: The results indicated dominant negative inhibitory activity of ΔNp63 and its potential role as an oncogene in breast cancer. ΔNp73L variant compared to d4TAp63, in tumoral and non tumoral breast tissues can act as dominant negative variant. Both variants (ΔNp73L and d4TAp63) with similar expression patterns to their respective longer variants (ΔNp63 and TAp63) and simultaneous expression with their variants are likely to be involved in strengthening the tumor suppressor activity in normal and tumoral breast cells. On the other hand, according to the expression patterns of ΔNp73L and d4TAp63 variants, they cannot be considered as molecular markers in the diagnosis of breast tumors. Keywords: Expression, TP63 gene, Breast cancer, Molecular markers, d4TAp63 variants, ΔNp73L variants. Received: Jun 8, 2013 Accepted: May 6, 2014
Marjaneh Kazemi, Dr Jafar Amani, Ali Ali Hatef Salmanian, Elahe Gheybi,
Volume 20, Issue 4 (10-2015)
Abstract

Background and Aim: Breast cancer is the second most common cause of death among women in the world. Normal mammary gland and breast carcinomas are under the control of regulatory factors, activators and inhibitors inside the breast tissue, as well as a number of growth factors, receptors and proteins outside the breast tissue. Levels of tumor-associated antigens can be used as a predictor in the treatment of this disease. Use of antibodies against MUC1 antigen which is over expressed in 90% of breast cancers is a modern method of treatment. MUC1 tumor antigen disturbs the function of E-cadherin as a cell adhesion molecule. The purpose of this study was to produce MUC-1 recombinant protein for early diagnosis of breast cancer. Material and Methods: A part of MUC-1 gene was amplified by PCR. Then it was cloned into plasmid pET28a in order to be expressed in prokaryotic system. Plasmid pET28a was entered into E.coli BL21DE3 using heat shock method. Cloning process by digestive enzymes and sequence determination were confirmed. Bacteria containing recombinant plasmids were induced by using IPTG and the protein expression was investigated by SDS-PAGE gel. Results: The gene was cloned in the plasmid and the method was confirmed by enzyme digestion and sequencing. Gene expression was confirmed by western blotting. Conclusion: A part of human recombinant MUC-1 gene was produced in E.coli bacteria which can be used as a suitable diagnostic candidate for breast cancer. Keywords: Cloning, MUC-1, Expression Received: May 7, 2014 Accepted: Jun 29, 2015
Narges Heydari, Hamed Tahmasebi, Behrooz Zeini, Sanaz Dehbashi, Dr Mohammad Reza Arabestani ,
Volume 23, Issue 1 (4-2018)
Abstract

Backgrounds and Aim: Staphylococcus aureus biofilms are involved in a multitude of serious chronic infections. Production of biofilms is a defensive-invasive process controlled and regulated by the aap and icaR genes. The expression levels of these genes play an important role in the formation of biofilm. The aim of this study was to investigate the expression of icaR and aap regulatory genes in clinical isolates of S. aureus resistant to methicillin and gentamicin.
Materials and Methods: In this analytical study, among 285 samples, we detected 100 isolates of methicillin resistant and 82 isolates of gentamicin resistant S. aureus. Resistant strains were evaluated for the presence of biofilm regulatory genes. The expression levels of regulatory genes were measured by real-time PCR method. We used SPSS software 16 for statistical analysis and also REST 2008 V3 software for analysis of quantitative results.
Results: Among 100 methicillin resistant and 82 gentamicin resistant isolates of S. aureus the highest expression levels of icaR and aap genes were detected in the smears obtained from the wounds and catheters. Moreover, a different pattern of gene expression was observed in multidrug resistant strains in comparison to the strains with lower rate of resistance. Also, there was a significant relationship between the presence and activity of regulatory genes and biofilm formation in different samples (p≤0 / 05).
Conclusion: Considering the frequency of biofilm producing strains of S. aureus in the smears from the catheters and wounds and also increased gene expression, appropriate therapeutic measures should be considered for methicillin and gentamicin resistant of S. aureus.
Keywords: Drug resistance, S. aureus, Virulence factors, Methicillin, Gentamicin, Gene expression.
 
Received: Aug 2, 2017     Accepted: Nov 6, 2017
Miss Marzieh Ghafari Farsani, Dr. Somayeh Reiisi, Dr. Maryam Peymani,
Volume 23, Issue 4 (9-2018)
Abstract

Background and Aim: Nowadays, breast cancer is the most common cancer among women in the world. Considering the high prevalence, identification of diagnostic markers can be useful for early diagnosis and cancer management. In recent years many studies have been conducted to determine the role of cytokines in cancers, but role of IL-37 in breast cancer has not been determined, yet. The aim of this study was to investigate IL-37 expression in tumor tissues from breast cancer patients and its relationship with pathologic and clinical symptoms.
Material and Methods: In this case-control study we examined 50 formalin-fixed paraffin embedded tumoral tissues from breast cancer patients and 50 non-tumoral adjacent tissues. After informed consent, clinical information of the samples were recorded. Total RNA was extracted and complementary DNA (cDNA) was synthesized. Then, the relative gene expression was determined by using quantitative real-time RT PCR (qRT-PCR) and evaluated by  method. Finally, the expression pattern was analyzed by statistical analysis.
Results: The results of this study indicated that, the mean relative expression of IL-37 in tumor tissues was significantly lower than that in adjacent healthy tissues and there was statistically a significant difference between them with a 95% confidence level (P = 0.003). The results also indicated a significant decrease in the expression of this cytokine in the tumoral tissues with sizes of >3cm (P = 0.02). The reduction in gene expression was clearly observed in the tumors of grade 2 and 3, which showed a significant relationship between gene expression and grade 3 tumors (P = 0.0001).
Conclusion: The results of this study revealed IL-37 can be regarded as a potentially sensitive and valuable biomarker for diagnosis of breast cancer and also tumor size determination. The results of this study can also be used in clinical and diagnostic  studies on breast cancer
Seyede Zahra Hasanpour, Mehdi Allah Bakhshian Farsani, Abbas Hajifathali, Mohammad Hossein Mohammadi,
Volume 23, Issue 6 (2-2019)
Abstract

Background and Aim: Acute lymphoblastic leukemia is the most common cancer in children and juveniles and is also seen in adults with lower frequency. Autophagy is a programmed catabolic process of the cell for destruction of damaged organs and proteins which is carried out by lysozymes. Disruption of autophagy leads to abnormalities in cellular processes associated with cancer.
Materials and Methods: In this study, we compared the expression of Beclin 1 and Atg 10 genes between 50 patients with B-ALL and 18 healthy subjects as our control group, by using RNA extraction and cDNA synthesis, and RT-PCR method. Data were analyzed by statistical tests.
Results: The majority of B-ALL patients showed a significant reduction in the Beclin1 and Atg 10 genes compared to control group (P <0.05) and the mean expression of the genes (2 -∆Ct ± SD) were 0.10 ± 0.49 and 1.01 ± 0.27 for ALL patients and control group and 0.15±0.44 and 1.07±0.85 for Atg10 and Beclin1 respectively. There was no significant correlation between expression levels of Beclin1and Atg10 in these patients (r =-0.013, P =0.926).
Conclusion: Considering the reduced expression levels in the essential genes of autophagy in ALL patients in our study and also other studies in this field, disruption of autophagy may be involved in leukomogensis
Dr Fahimeh Ghasemi, Dr Fatemeh Nikumanesh, Dr. Alireza Zomorodipour,
Volume 28, Issue 2 (5-2023)
Abstract

Background and Aim: The current treatment for hemophilia B is replacement therapy, which involves the intravenous infusion of human coagulation factor IX (hFIX) purified from plasma or a recombinant form produced in mammalian cells. In this study, using a bicistronic expression system, the stable expression of the hFIX in a serum-free and suspension-adapted Chinese hamster ovary cell line (CHO-s) was investigated.
Materials and Methods: A DNA fragment consisting of hFIX, Internal Ribosome Entry Site (IRES) and Enhanced Green Fluorescent Protein (EGFP) nucleotide sequences was cloned into pcDNA3.1 expression plasmid under the control of Cytomegalovirus (CMV) promoter. The bicistronic plasmid was then linearized using BglII restriction enzyme and transfected into CHO-s cells. The transfected cells were treated with geneticin for 14 days. The culture medium of the stable cells was then collected and the expression level of the hFIX were examined using western blotting and ELISA. The coagulation activity was also evaluated by the chromogenic method.
Results: The recombinant CHO-s cells resistant to geneticin were observed under a fluorescence microscope in green color, which indicated the expression and accumulation of the EGFP in the cytoplasm of the cells. The results of Western blotting confirmed the expression and secretion of the hFIX into the culture medium. The amount of the secreted hFIX was 150 ng/mL/106cells with a coagulation activity of 5.6 ±0.2 mU/mL.
Conclusion: Our findings demonstrated that this bicistronic expression system could simultaneously produce EGFP and hFIX in CHO-s cells. This expression system facilitates selection and isolation of hFIX-expressing cells.
Dr. Mahsa Rasekhian, Dr. Maryam Pourjalili, Dr. Omid Tavallaei,
Volume 28, Issue 4 (10-2023)
Abstract

Background and Aim: Conventional treatments have shown little success in cancer treatment. Using antibody fragments conjugated with specific proteins and targeting specific receptors is a new strategy for producing anticancer drugs. Despite the advantages of prokaryotic hosts, protein expression in the form of inclusion bodies (cytoplasmic) has been challenging and leads to subsequent difficulties. Production of proteins as soluble forms will significantly help to solve this problem.
 The primary purpose of this study was to clone and express Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) conjugated with anti CD20 single chain fragment variable (scFV) region of anti-CD20 antibody as a soluble protein using a small ubiquitin-related modifier (SUMO) fusion protein system in E. coli as a new method for optimal production of anticancer drugs.
Materials and Methods: Appropriate primers for a previously designed DNA sequence encoding the fusion protein were used to amplify the fragment. The amplified fragment was sub-cloned downstream of the SUMO tag in pSUMO vector. Once confirmed by standard methods, the recombinant plasmid was transformed into E. coli strain BL21 (DE3). Expression was induced by isopropyl β- d-1-thiogalactopyranoside. The expressed proteins were assessed by SDS-PAGE and then isolated by affinity chromatography. Western blotting was used to confirm protein expression.
Results: The results showed the DNA of the recombinant fusion protein was sub-cloned correctly, and the relevant analyses indicated that the expression process was successful. The recombinant fusion protein was confirmed by appropriate analysis.
Conclusion: The production of recombinant proteins in a soluble state in the prokaryotic host E. coli can reduce the costs of the downstream process. Production of soluble antiCD20 scFV-TRAIL fusion protein can be considered a proper method to produce recombinant protein in a soluble state in the E. coli system and can decrease the cost of downstream processes.
Ms Sara Rafi Taheri, Ms Arezoo Farhadi, Ms Zahra Shahsavar Haghighi, Dr Ebrahim Hazrati, Dr Foad Heidari Mohammad, Dr Peyman Aslani, Dr Mojgan Mohammadimehr, Dr Reza Heidari, Dr Javad Behroozi, Mrs Ali Zarei,
Volume 29, Issue 2 (5-2024)
Abstract

Background and Aim: Gastric carcinoma (GC) is one of the most common human cancers. Many genes have been analyzed in an attempt to better understand the process of gastric carcinogenesis, however, the underlying mechanism of gastric carcinogenesis is still poorly understood. Dermatopontin (DPT) is an extracellular matrix protein, which regulates multiple physiological processes. The present study aimed to evaluate DPT gene expression in GC.
Materials and Methods: Biopsies of 50 surgically-excised GC tissue specimens and corresponding adjacent normal tissues were examined by real-time PCR. Then, using a bioinformatics analysis we determined DPT gene expression in two different cohorts of GC patients. To determine the effect of DPT expression levels on survival outcome, a Kaplan–Meier analysis was performed. For a comprehensive analysis, DPT gene expression was evaluated in 16 different cancers.
Results: RT-PCR demonstrated that DPT gene expression was decreased in gastric cancer tissues, compared to that in the normal stomach tissues. Mean DPT expression value was significantly lower in different stages of GC. Further, survival analysis revealed that the mRNA expression of DPT is positively correlated with overall survival of GC patients. A relationship was found between DPT expression and primary size of tumor. Pan-cancer analysis in 16 tumor types showed that DPT expression was lower in tumor tissue than in the adjacent normal tissue.
Conclusion: These findings suggested that the decrease in DPT gene expression in gastric tissue may play an important role in gastric carcinogenesis.
 
Mr Moein Taghvaei, Ms Zeynab Askari, Mr Alishir Haidari, Dr Saeedeh Samareh Moosavi, Dr Farimah Beheshti, Dr Seyed Mohsen Saleh, Dr Saeedeh Askarian,
Volume 30, Issue 2 (5-2025)
Abstract

Background and Aim: The use of lithium, a mood-stabilizing drug, is a common treatment for bipolar disorder. Discontinuing lithium during pregnancy can be dangerous, as it may cause manic or severe depression in the mother. Despite the positive effects of lithium, it is important to study the molecular effects of this drug on infants. This study aims to investigate the expression of the miR-132 gene in the neonates of rats whose mothers were exposed to lithium during pregnancy.
Materials and Methods: In this study, experimental female mice received lithium carbonate at a concentration of 30mg/kg in their drinking water daily during pregnancy. The newborns were then divided into two control and experimental groups. When the neonates reached 60 days of age, their hippocampus and blood serum were isolated for molecular analysis. Total RNA was extracted from the blood and serum, followed by cDNA synthesis. The expression of miR-132 was evaluated using qRT-PCR and compared with the control group using the Pfaffl method.
Results: The expression of miR-132 in the hippocampal tissue and serum samples of infants whose mothers received lithium during pregnancy showed a significant decrease compared to the control group. The down-regulation of miR-132 expression was observed to be more pronounced in serum samples compared to hippocampal tissue samples.
Conclusion: The decreased expression of miR-132 in the hippocampus and blood serum of newborn rats exposed to lithium during pregnancy indicates the influence of lithium on gene expression. This miRNA showed potential as a valuable indicator for detecting the effects of drug exposure on offspring. However, identifying specific miRNA markers for bipolar disorder is a challenging quest, and further studies are required.
 

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مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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