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Showing 2 results for Amphetamine
Dr Shabnam Mohammadi, Zahra Pakrouh, Mahin Teimouri, Saedeh Haji Pour, Dr Mehdi Karimi, Dr Maryam Mohammadi, Dr Ali Mohammad Pour, Mohammad Hossein Tani Pour, Dr Isaac Hashemi, Dr Mohammad Sookhtanlu, Dr Kamyar Tavakkoli, Fatemeh Mohammadzadeh,
Volume 20, Issue 4 (10-2015)
Abstract
Background and Aim: Methamphetamine causes damage in different body organs. The aim of this study was to investigate the effects of methamphetamine on morphometric changes and biochemical parameters in kidney of adult mice.
Material and Methods: In this experimental study, 56 mice were randomly divided into 7 groups: control and 6 experimental groups. Experimental groups 1, 2 and 3 received methamphetamine 5, 10 and 20 mg/kg respectively for 10 days. Experimental groups 4, 5 and 6 received methamphetamine (5, 10 and 20 mg/kg) respectively for 40 days. After 10 and 40 days, kidneys of the mice of the experimental groups were removed and after tissue processing and imaging, volume of kidney and glomerulus were measured by using cavalieri's principle. In addition blood urea and creatinine levels were measured. Using SPSS software, data were analyzed by means of ANOVA.
Results: Histopatological study showed that methamphetamine increased renal damage in a dose dependent manner. High doses of methamphetamine caused a moderate lobulation, glomerulosclerosis and kidney congestion. There was also a significant difference between the experimental (groups 3 to 6) and control groups in regard to the volumes of kidney and glomerulus (P=0.000).
Significant differences were found in the level of urea in group 6 and creatinine levels in all experimental groups compared to those of control group (P=0.000).
Conclusion: Use of 10 mg/kg of methamphetamine for 40 days led to destructive effects on kidney tissue. Also, it increased serum levels of urea and creatinine.
Keywords: Methamphetamine, Kidney, Mice.
Received: Dec 31, 2014 Accepted: Jun 6, 2015
Mohammad Ahmadia Khosroshahi, Parvin Gharbani,
Volume 26, Issue 3 (7-2021)
Abstract
Background and Aim: Nowadays, drug abuse is a major problem in societies, and detection of drugs in urine is very important. In general, immunochromatography (ICG) and thin-layer chromatography (TLC) are routine methods for the detection of drugs in urine. These methods are rapid and economical, but their accuracy rates for the detection of drugs at lower concentrations are low (< cut off). Morphine, amphetamine, and methamphetamine are conventional drugs that are widely used. The purpose of this study was to compare immunochromatography , thin layer chromatography (TLC), and gas chromatography (GC) methods for detection of morphine, amphetamine, and methamphetamine in the spiked healthy human urine samples and urine of addicted people. We also compared their detection limits with one another.
Materials and Methods: This was an experimental study and included urine samples obtained from healthy and addicted people referring to the laboratory of the 7th of Tir marriage counseling center in East Azerbaijan Province, in June 2016. After the collection of urine samples, samples obtained from healthy people were spiked with various concentrations of morphine, amphetamine, and methamphetamine. Then, all samples were tested by immunochromatography, TLC, and GC methods for the detection of morphine, amphetamine, and methamphetamine.
Results: Results showed that ICG and TLC methods can not detect lower concentrations (< cut off) of morphine, amphetamine, and methamphetamine. While, GC can easily detect them in urine samples, even in lower concentrations (< cut off), and has a high detection limit and accuracy rate.
Conclusion: It can be concluded GC method is a powerful and accurate technique for the detection of drugs in urine samples.
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