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Showing 31 results for amini
Dr M.r Palizvan , E Ghaznavi, A Amini Komijani , Volume 10, Issue 2 (9-2005)
Abstract
ABSTRACT Background and Aim: In this study the effect of voltage dependent calcium channels of the CA1 region of the hippocampus on the pentylenetetrazol (PTZ) induced kindling and resultant learning deficits were investigated in rats. Materials and Methods: In this study the rats were divided into three groups. In the control group artificial cerebrospinal fluid (ACSF) was infused bilaterally into the hippocampus. In the kindled group, 20 min after infusion of ACSF, kindling was established in rats by injection of subconvulsive dose (37.5 mg/kg i.p.) of PTZ intraperitoneally. The rats were kept under observation for 20 minutes and the convulsive responses were graded on the basis of a 5-scale criterion. In the test or verapamile group, verapamile, were injected in the hippocampus (4 m g/4 min) and after 20 min Kindling was established in rats by use of PTZ. One month after induction of kindling spatial learning and memory were tested by Morris water maze in all three groups. Results: The�results of this study were suggestive of a significant decrease of spatial learning in the rats of the test group. Conclusion: In this research, intera-hippcampal injection of verapamil significantly impaired spatial learning in rats. Key words: Pentylenetetrazol, Kindling, Verapamil, Hippocampus, Spatial learning
Dr R Hosseini Doust, Dr A Mohabati Mobarez, Dr M Amini, F Haghi Tumetri, Volume 13, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2008)
Abstract
ABSTRACT Background and aim: The antibiotic resistance and also harmful side effects of treatments processes are among the most fundamental reasons of treatment failures. Therefore the global efforts are focused on subsidiary and more successful treatment protocols. The aim of this study was to assess inhibitory effect of one probiotic regime on H. pylori isolated from peptic ulcer patients. Material and Methods: Forty species of H. pylori were isolated from stomach biopsy samples from 140 patients with gastritis. After culturing biopsy samples in brucella blood agar and identification of the bacteria by standard complementary tests, antibiotic sensitivity test was performed by disk diffusion and E-Test methods. Antibiotic resistance to metronidazole, claritromycin, amoxicillin, and tetracycline was checked for all H. pylori isolates. H. pylori species were cultured in brucella broth medium and then the isolates were co-cultured with L acidophilus for assessment of antagonistic effect of L acidophilus on H. pylori growth. Results: Among 4 5 species, 25 (55 .5 %) showed antibiotic resistance. The resistance rates to metronidazole, claritromycin, amoxicillin and tetracycline were (65 %), (33%), (22%) and (20%) respectively. Lacto bacillus acidophilus prevented growth of 26 (57.7%) H. pylori- species among which, 68% were antibiotic sensitive and 31.8% antibiotic resistant. Conclusion: Although growth of half of H.pylori isolates were inhibited by Lactobacilli species, the proportion of unaffected isolates is considerable. The results of this study were almost compatible with those of other studies. Checking more isolates under standard laboratory conditions is necessary to determine the exact inhibitory effect of lactobacillus. At the present time the inhibitory effect of lactobacillus on some enteric pathogens has been recognized, but more studies are required to understand the mechanism and factors involved in such inhibitory effect.
Amir Amini, Dr Mohammad Reza Kordi, Dr Abas Ali Gaini, Abbas Ahmadi, Heresh Ayoubian, Fariba Lahoorpour, Volume 15, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2011)
Abstract
ABSTRACT
Background and Aim: Blood coagulation and fibrinolysis are two main components of homeostasis. Lack of balance between activities of these systems and their controlling mechanisms can lead to bleeding and clot formation. There are controversial reports about the effect of physical activities on these systems. The effect of aerobic exercise on coagulation and fibrinolytic factors in inactive old men has not been determined in the previous studies and these factors have direct relationship with cardio vascular problems. In this study we evaluated the effect of this kind of exercise on some of the factors of these systems.
Materials and Methods: This was an experimental study. Sampling method was convenient. For this purpose, 16 male volunteers with age range of 60-70 years were divided randomly into 2 groups: control and aerobic groups (n1=n2=8). Aerobic group performed physical exercise with ergometer in the first 2 weeks with 65%HRmax intensity for 30 minutes, and the next 2 weeks with 75% HRmax intensity for 35 minutes. Blood samples were taken 24 hours before the first, and 24 hours after the last training sessions. Fibrinogen, prothrombin time (PT), partial thromboplastin time (PTT), platelet counts and D-dimer were measured in both groups before and after training. Differences between the above mentioned factors in each person, before and after training, and between test and control groups were analyzed by means of t-test.
Results: Data analyses showed that aerobic training induced significant decrease in fibrinogen, PTT, PT and platelets counts (p<0.05), and significant increase in D-dimer (p<0.05). These changes were not observed in the control group (p>0.05).
Conclusion: According to the findings of this study we concluded that 12 sessions of aerobic training can decrease coagulation factors and increase fibrinolytic factors of the blood and can prevent vascular thrombosis.
Key words: Aerobic, Coagulation, Fibrinolytic, Inactive aged men
Conflict of Interest: Nill
Received: Nov 15, 2010 Accepted: Dec 26, 2010
Negin Mollasalimi, Fatemeh Ghanbari, Dr Esmael Izadpanah, Dr Himan Khosropanah, Dr Amin Rostami, Abas Ahmadi, Soleiman Kurd, Amir Amini, Ashkan Tavakoli, Dr Kambiz Hasanzadeh, Volume 15, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2011)
Abstract
ABSTERACT
Background and Aim: Allium porrum L. is a plant from the Liliaceae family and has been used in Iranian foods as flavor. It has been used in traditional medicine in different ways. In the western parts of Iran, people believe that the fresh Allium juice can inhibit epistaxis. The aim of this study was to evaluate the effects of methanolic and n-hexanic extracts of Allium porrum L. on coagulation tests in human beings in vitro.
Materials and Methods: The methanolic and n-hexanic extracts of Allium porrum L. were prepared using continuous extraction method. Effect of different concentrations of extracts on prothrombin time (PT), partial thromboplastin time (PTT) and clotting time (CT) was evaluated. The result was analyzed by analysis of variance.
Results: The results of the present study showed that the methanolic extract of Allium porrum increased PT and PTT but failed to change the clotting time. In the presence of N-hexanic extract, clotting time increased but PT decreased.
Conclusion: The results of this study indicated that Allium porrum extracts had a significant anti-coagulatory effect. However, some of the findings of this study are controversial, and further studies on animal models are needed in order to clarify the possible mechanisms.
Key words: Allium porrum L., Prothrombin time, Partial thromboplastin time, Clotting time.
Conflict of Interest: Nill
Received: July 19, 2010 Accepted: Dec 20, 2010
Dr Sabrieh Amini, Dr Fardin Fathi, Dr Bahram Nikkho, Dr Heshmatollah Sofimajidpour, Dr Jafar Mobaleghi, Volume 16, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2011)
Abstract
ABSTRACT
Background and Aim: Here we examined the expression of self renewal regulatory factors such as, Esrrb, Tcl1, Tbx3 and Dppa4 in several tissue samples of cancers and cancer cell lines. These genes are required for efficient self renewal of embryonic stem cells.
Material and Methods: Caco2, HT-29, HT1376, Ln Cap, and HepG2 cells were cultured in T25 flasks. Considering the clinical and laboratory findings, human tumor samples were obtained under direct supervision of the medical specialists. Then we evaluated expression of self renewal genes (Tbx3, Tcl1, Esrrb, Dppa4) by reverse transcriptase polymerase chain reaction (RT-PCR) in the above mentioned cells and human tumor samples. To confirm the validity of the laboratory tests, we studied negative control samples and internal control genes.
Results: Our data revealed the expression of self renewal genes (TCL1, TBX3, ESRRB and DPPA4) in bladder, liver, prostate and colon cancers and also cancer cell lines.
Conclusion: Colon, liver, prostate and bladder cancer cells can express TCL1, TBX3, ESRRB and DPPA4 genes, which are specific markers of stem cells. Therefore in malignant cells of the above mentioned cancers, some cells have the characteristics of stem cells and can play an essential role in the proliferation of malignant cells.
Key words: Cancer stem cells Self renewal gene Colon cancer Prostate cancer Bladder cancer
Conflict of Interest: Nill
Received: Oct 14, 2010 Accepted: Dec 28, 2010
Dr Mohammad Taghi Hedayati, Sadegh Khodaveisy, Dr Behrooz Davari, Dr Ghasem Zamini, Volume 16, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2012)
Abstract
ABSTRACT
Invasive aspergillosis (IA) is a major cause of morbidity and mortality in severely immuno-compromised patients. Despite the advances made in diagnostic methods, accurate diagnosis of IA is not easy. At the present time, in Iran, the diagnosis is most often made based on a combination of clinical and abnormal radiologic findings, which are nonspecific, and the treatment is often given without establishing the diagnosis. Considering the invasive and progressive nature of the disease, if proper diagnostic methods are not used, control of the disease will be difficult. Therefore establishing the diagnosis of IA at an early stage by non-invasive and specific methods is necessary for early successful treatment. The detection of circulating galactomannan (GM) antigen in serum, bronchoalveolar lavage fluid, urine, and cerebrospinal fluid and tissue has become an important method for the early diagnosis of IA. Recent data have indicated that this test has a high specify and sensitivity and is more valuable than other methods such as culture and CT scan. In general this method is non-invasive, time-saving and specific which permits early treatment of the disease and helps physicians to select the proper treatment and other clinical measures. Certainly, well designed prospective studies with systematic sampling and use of accepted definitions are required to compare the efficiency of antigen detection in different samples and population.
Kaywords: Invasive aspergillosis, Galactomannan, Diagnosis
Received: May 14, 2011 Accepted: Nov 5, 2011
Conflict of interest: Nill
Naaman Ardalan, Dr Mohammad Abdi, Dr Bahareh Rahimian Zarif, Dr Amir Amini, Farzam Meamari, Erfan Haydari, Abbas Ahmadi, Volume 18, Issue 2 (Scientific Journal of Kurdistan Universiy of Medical Sciences 2013)
Abstract
ABSTRACT
Background and Aim: HTLV-I is the etiological cause of adult T-lymphocytic leukemia (ATL) and a chronic degenerative neurologic disorder, called tropical spastic paraparesis (TSP). HTLV infection can be transmitted through different ways: from mother to child or fetus, sexual intercourse, transfusion of contaminated blood or blood products, and sharing contaminated syringe needles. As the presence of these infections in high risk groups can be an approximate indicator of their prevalence in the society and blood donors, in this study was tried to find prevalence of HTLV in HIV positive or negative intravenous drug users (IVDU patients with major thalassemia, and hemodialysis patients, in Sanandaj.
Materials and Methods: This descriptive study included 351 cases: 130 HIV positive and 110 HIV negative intravenous drug users (IVDU), 46 cases of major thalassemia, and 65 hemodialysis patients. All participants completed written informed consent forms. After obtaining blood samples and serum separation, all specimens were kept in freezer at -20oC up to the time of analysis. Serum samples were screened for measurement of the titers of HTLV I&II antibodies by Dia-Pro ELISA kits, manufactured in Italy. Positive and suspicious reactions were reanalyzed. For confirmation of positive and suspicious reactions, samples with one positive reaction were examined by use of western blot kid (HTLV blot 2.4, manufactured by MP Diagnostics in Switzerland). Data were entered into SPSS 16 software and the prevalence rates of these viruses were obtained by using frequency distribution table.
Results: The results of this study showed that one HIV positive, one HIV negative patient and another patient with major thalassemia were HTLV I positive (0.85%). None of the hemodialysis patients had antibody against HTLV type 1. We did not find any antibody against HTLV type 2 in our study.
Conclusions: The prevalence rate of HTLV (types 1 and 2) among these high risk groups was not high in Sanandaj. This may reflect its low prevalence in general population and in blood donors. However, it is necessary to take preventive measures to reduce its spread. To assess the exact prevalence rate we recommend screening of all donated blood samples and general population.
Conflict of Interest: Nil
Received: May 24, 2012 Accepted: Oct 11, 2012
Mehdi Soleimani, Amir Amini, Abas Ahmadi, Dr Sirvan Atashak, Ayoob Mehdivand, Elias Kawsari, Alireza Shamsoddini , Behzad Bazgir, Volume 18, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2013)
Abstract
ABSTRACT
Background and Aim: Cardiovascular diseases (CVD) are among the most important causes of death and disability all around the world. Blood cells, especially platelets, may play a crucial role in pathophysiology of these disorders. Considering the increased risk of thrombosis after acute physical activities, and the role of the platelets in these disorders, many nutritional approaches had been evaluated for the prevention of thrombosis. Recently, the effects of cocoa consumption on hemostasis have recently attracted the attention of many researchers. In this study we evaluated the effects of cocoa consumption on platelet count, mean platelet volume (MPV), and platelet distribution width (PDW), during one session of incremental exhausting aerobic exercise in male soccer players.
Materials and Methods: This semi-experimental study included 20 healthy volunteer male soccer players (age: 22±1years BF%: 22.5±1.2 VO2max:52.6±1.5 ml.kg-1.min). After written consent, all subjects performed Bruce Test within two successive weeks. After the first blood sampling (stage one), 0.5 mg/kg of placebo (0.5 g cocoa powder in 300 ml of 4% sucrose solution) or cocoa solution (18.75 g cocoa powder in 300 ml 4% sucrose solution) was randomly given to the subjects. All cases performed Bruce Test two hours later. Blood samples were collected just before Bruce Test (second stage), immediately after Bruce Test ( third stage) and 1 hour after Bruce Test (fourth stage). After preparation of peripheral blood smears, platelet count, MPV and PDW were measured by Mindray cell counter. Using spss 16, data were analyzed by means of two-factor analysis of variance (ANOVA) and Bonfferoni test at the level 0.01.
Results:Our results indicated a significant decrease in platelet count, MPV, and PDW after cocoa consumption (P<0.01). In addition, there was a significant difference between the 2 groups in these values after Bruse Test (P<0.01). However, in spite of significant increase in platelet count, MPV, and PDW after Bruse Test, cocoa consumption 2 hours before the test decreased these values significantly (P<0.01).
Conclusion: Cocoa consumption before exhaustive physical exercises may prevent exercise induced increase in the platelet indices hence, potentially can prevent cardiovascular and thrombotic events and sudden death in the athletes.
Key words: Cardiovascular disease, Platelet, Cocoa, Exhaustive exercise, Bruce Test
Received: Jul 25, 2012 Accepted: Sep 10, 2012
Conflict of interest: None declared
Dr Afshin Maleki, Hiwa Daraei, Hasan Amini, Pegah Bahmani, Volume 19, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2014)
Abstract
Background and Aim: Nowadays, the increasing concentration of pollutants in water resources, especially ground water, has been a matter of concern. Nitrate has been considered as a chemical indicator of water pollution for a long time. The aim of this study was to determine the chemical characteristics of drinking water and changes of nitrate in drinking water in Diwandareh villages.
Materials and Methods: This was a cross-sectional study to determine the quality of drinking water of 30 villages in Divandareh. A total of 60 samples were obtained in two phases from water supplies and were analyzed according to the standard methods. Descriptive statistics, Piper, Schuler and Wilcox diagrams were used to interpret the results.
Results: The results showed that apart from nitrate, the concentrations of the other chemical quality parameters were below the standard limits. The mean and standard deviation of nitrate during the first and second stages of sampling in water sources were 26.13 ± 25.61 mg L-1 and 28.47 ± 29.80 mg L-1, respectively. According to Schuler diagram, the drinking water quality was moderate to good.
Conclusion: Although the mean nitrate concentration in 80% of samples was lower than the latest national standard for nitrate, continuation of the current trend, particularly in the agricultural activities and uncontrolled use of groundwater resources in agriculture will change and lower the quality of water. Therefore, monitoring of agricultural practices and fertilizer use are necessary.
Keywords: Water quality monitoring, Water recourse, Water quality indices, Nitrate.
Received: Dec 28, 2013 Accepted: March 1, 2014
Halaleh Hassanzadeh, Arman Rahimmi, Dr Sabrieh Amini, Dr Kambiz Hassanzadeh, Volume 20, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2015)
Abstract
Background and Aim: Recent studies have shown that oxidative stress has a critical role in the pathophysiology of Parkinson’s disease. Frontal cortex receives a great amount of dopaminergic neurons from nigrostriatal pathway and is one of the brain regions which aredamagedin Parkinson’s disease. On the other hand anti-oxidant properties of N-acetylcysteine have been proven to occur via fortifying glutathione. Glutathione is one of the main intracellular anti-oxidant systems. Therefore our study was aimed to evaluate the effect of N-acetylcysteine in the management of Parkinson’s disease. Materials and Methods: Male Wistar rats with age range of 10-12 months and weights of 400±50g received rotenone (2.5 mg/kg/48h ip) to induce Parkinson’s disease model. NAC (25 or 50 mg/kg/48h ip) was administered one hour before rotenone injections. In order to measure the motor symptoms and verify the development of the model, rotarod test was performed. Moreover the frontal cortex parkin level, one of the crucial proteins in Parkinson’s disease, was measured using western blot technique. Results: The results indicated that N-acetylcysteine could prevent decline in the motor performance inrotarod test. In addition frontal cortex parkin level was significantly decreased in rotenone received animals while N-acetylcysteine prevented the reduction of parkin in this region. Conclusion:Our results indicated that that N-acetylcysteine could prevent the development of Parkinson’s disease in this model which is probably due to its anti-oxidant properties. Keyword: Parkinson’s disease, Rotenone, N-acetylcysteine, Parkin, Frontal cortex Received: Aug24, 2014 Accepted: Dec 7, 2014
Halaleh Hassanzadeh, Arman Rahimmi, Dr Sabrieh Amini, Dr Kambiz Hassanzadeh, Volume 20, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2015)
Abstract
Background and Aim: Recent studies have shown that oxidative stress has a critical role in the pathophysiology of Parkinson’s disease. Frontal cortex receives a great amount of dopaminergic neurons from nigrostriatal pathway and is one of the brain regions which aredamagedin Parkinson’s disease. On the other hand anti-oxidant properties of N-acetylcysteine have been proven to occur via fortifying glutathione. Glutathione is one of the main intracellular anti-oxidant systems. Therefore our study was aimed to evaluate the effect of N-acetylcysteine in the management of Parkinson’s disease.
Materials and Methods: Male Wistar rats with age range of 10-12 months and weights of 400±50g received rotenone (2.5 mg/kg/48h ip) to induce Parkinson’s disease model. NAC (25 or 50 mg/kg/48h ip) was administered one hour before rotenone injections. In order to measure the motor symptoms and verify the development of the model, rotarod test was performed. Moreover the frontal cortex parkin level, one of the crucial proteins in Parkinson’s disease, was measured using western blot technique.
Results: The results indicated that N-acetylcysteine could prevent decline in the motor performance inrotarod test. In addition frontal cortex parkin level was significantly decreased in rotenone received animals while N-acetylcysteine prevented the reduction of parkin in this region.
Conclusion:Our results indicated that that N-acetylcysteine could prevent the development of Parkinson’s disease in this model which is probably due to its anti-oxidant properties.
Keyword: Parkinson’s disease, Rotenone, N-acetylcysteine, Parkin, Frontal cortex
Received: Aug24, 2014 Accepted: Dec 7, 2014
Abdollah Hassanzadeh, Dr Kaveh Shahvaisi, Dr Kambiz Hassanzadeh, Dr Esmael Izadpanah, Dr Arius Amini, Dr Mohammad Raman Moloudi, Volume 20, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2015)
Abstract
Background and Aim: Rebamipide is a quinolone derivative drug, which stimulate prostaglandin production and mucus secretion. Despite gastric mucosal protection and ulcer healing effect, it has low solubility and low permeability. An efficient way to increase drug permeability and solubility is to use the polymeric carrier and nanoparticles which effectively increase drug delivery. The aim of this study was to evaluate the effect of rebamipide and encapsulating rebamipide with chitosan capsule on inflammatory mediators in rat experimental colitis.
Methods: In this study, 36 male wistar rats were randomly divided into 6 groups (n= 6) including rebamipide-chitosan, rebamipide, chitosan, positive control (hydrocortisone), control (rebamipide vehicle) and sham operated. After 36 h fasting, colitis was induced with 2 ml of acetic acid 4% (except sham operated groups that received saline). After 6 days, animals sacrificed and samples of colon tissues were obtained for MDA, NO and TNF-α levels assessment and determination of MPO and SOD activity. The results were analyzed by one way ANOVA.
Results: Encapsulating rebamipide with chitosan capsule not only significantly reduced TNF-α and NO (P <0.03) compared with rebamipide solution, but also diminished other anti-inflammatory mediators and increased antioxidant capacity compared with other groups.
Conclusion: Encapsulating rebamipide with chitosan capsule can increase the effectiveness of this drug however more studies are needed to support this hypothesis for clinical use.
Key words: Chitosan capsule, ulcerative colitis, Rebamipide, TNF-α and NO.
Received: Feb 23, 2015 Accepted: May 11, 2015
Dr Seyed Saman Talebi, Dr Manochehr Amini, Dr Mostafa Qorbani, Volume 20, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2015)
Abstract
Background and Aim: According to the recorded data on the Health Ministry database, a better understanding of clinical and epidemiological factors associated with ESRD can be helpful for decision making about the treatment and preventive interventions with the aim of public health promotion and development of health programs. This study aimed to evaluate the prevalence of ESRD and dialysis-related factors from 2000 to 2009.
Material and methods: In this cross sectional study, data were obtained from specific diseases office of the Ministry of Health and Medical Education of the Islamic Republic of Iran from 2000 to 2009. There was no limitation on patient entry. Finally data were analyzed using SPSS software.
Results: This study included 12797 patients (57.07% male and 42.57% female) from 2000 to 2009. The overall incidence of ESRD in men (compared to the gender distribution in the normal population) with a mean age of 58.8 (SD = 17.61) was higher in comparison to women. The most common etiologies were diabetes (25.5%), and hypertension (23.3%) respectively. There were no significant changes in diabetes and hypertension frequencies in the patients between 2000 and 2009. No significant relationship was found between blood groups and ESRD. Prevalence of anemia was higher in the women with ESRD and hemoglobin levels were directly correlated with educational status (P <0.001).
Conclusion: The incidence of ESRD has been on the increase during this study. Also we found a decrease in the middle and an increase at the end of the study in the prevalence of HIV which calls for further assessments.
Keywords: Iran, ESRD, Hemodialysis, Prevalence, Anemia
Received: Sep 28, 2014 Accepted: Jun 21, 2015
Seyedeh Sara Moosavi, Kambiz Davari, Sabrieh Amini, Volume 20, Issue 6 (Scientifis Journal of Kurdistan University of Medical Sciences 2016)
Abstract
Background and Aim: In recent years, the production of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of bacteria in particular E.coli has been on the rise. Production of beta-Lactamase in E.coli has caused many problems in the treatment of the patients. The CTX-M-2 gene is one of the several factors producing resistance due to ESBL. The aim of this study was to evaluate the antimicrobial susceptibility to beta-lactam antibiotics and investigation of the CTX-M-2 gene level in E. coli isolated from urine samples.
Material and Methods: In this study, 260 UTI samples were collected from medical centers in Sanandaj and 100 E.coli isolates were collected and confirmed by biochemical tests. Then susceptibility test to 11 selected antibiotics were performed by disk diffusion method and the ESBLs producing strains were identified by the combined disk method. Using PCR method, ESBLs positive strains were examined for the presence of CTX-M-2 gene.
Results: The results of the phenotype tests showed that out of 100 E.coli strains, 27 (27%) were ESBLs producing. Also, PCR showed that among these 27 strains, 2 (7.4%) strains contained CTX-M-2 gene.
Conclusion: Considering the high rate of resistance to the third generation cephalosporins, careful antibiogram tests is an inevitable necessity before prescribing antibiotics for the treatment of infections caused by ESBLs producing organisms.
Keywords: E.coli, ESBLs, CTX-M-2.
Received: Aug 16, 2015 Accepted: Nov 8, 2015
Dr Ghasem Zamini, Dr Mohammad Bagher Khadem-Erfan, Dr Mohammad Nasim Karimi, Ashkan Faridi, Volume 21, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2016)
Abstract
Background and Aim: Oxyuris or pin worm is a type of roundworm (nematode genus Enterobius) which has small, thin, white slender shape. The size of adult male is about 2 to 5 mm and adult female measures 8 to 13 mm in length. The purpose of this cross- sectional study was to determine the prevalence of oxyuriasis and its correlation with clinical manifestation and allergic symptoms among children between 1 and 6 years of age in "Oraman Takht" region of Marivan, in Iran. "Oraman Takht" region consists of 10 villages. A total number of 845 children between 1and 6 years of age, were living in this area.
Material and Method: Using systematic random sampling method, 338 children were enrolled in the study. Data were collected by means of a special questionnaire, microscopic examination of scotch tape test specimens and peripheral blood smear. Data about parents, education and occupation and children's sex and age, were recorded in a questionnaire for every subject. Peripheral blood smear were obtained from all children and was examined by a hematologist for presence of eosinophilia. Scotch tape tests were prepared by the parents after necessary instructions, and examined for the presence of oxyuriasis ova by a parasitologist.
Results: Among 340 children in our study 139 (%41.1) were infected by E. vermicularis. The prevalence rate of infection was %37.4 in the boys and %45.3 in the girls. There was not a significant relationship between E. vermiculris infection and its clinical manifestation and allergic disease symptoms. 22 (%6.4) children showed eosinophilia in their peripheral blood smears, among them 17 (%77.3) were infected by E. vermicularis, too. There was a significant relationship between eosinophilia and E.vermicularis infection (p‹0.001).
Conclusion: Considering the results and importance of this subject and for better control and prevention of parasitic infection, we suggest necessary training and education for the people in regard to the life cycle of the parasite, transfer and prevention of infection. Measures for improvement of economical status, public and personal healths are recommended. Finally all the household members should be treated at the same time.
Key words: Enterobius vermicularis, Enterobiasis, Oxyuroidea, Eosinophilia, Children, Iran.
Received: Apr 11, 2016 Accepted: Jun 15, 2016
Najmeh Aminizadeh, Dr Taki Tiraihi, Dr Seyed Alireza Mesbah-Namin, Dr Taher Taheri, Volume 21, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2016)
Abstract
Background and Aim: The number and potential of proliferation and differentiation of bone marrow mesenchymal stromal cells decreases with age. These changes reduce efficacy of autologous transplantation in old people. The purpose of this study was to evaluate the effect of sodium selenite on telomere length and telomerase activity of bone marrow stromal cells (BMSCs) in aged rats.
Material and Methods: The BMSCs collected from aged male rats were cultured and treated with different concentrations of sodium selenite for 72 h. We evaluated the effect of sodium selenite on the proliferation potential of these cells using trypan blue exclusion test. Then, we evaluated the efficacy of the effective concentration of sodium selenite on telomerase activity, telomere length and the related telomerase gene expression. Telomerase activity was assessed byPCR-ELSA method and telomere length, and its related gene expression was assessed by the real time PCR technique.
Results: Use of sodium selenite at the concentration of 100nM led to significant increase in the proliferation of BMSCs and decreased telomere length in the aged rats compared to the control group, although the difference was not significant. Telomerase activity and the related telomerase gene expression did not show any change.
Conclusion: Sodium selenite improved proliferation of BMSCs of the aged rats.
Key words: Sodium selenite, Aging, Mesenchymal stromal cells, Telomere, Telomerase.
Received: Dec 12, 2015 Accepted: Apr 24, 2016
Dr Manoochehr Amini, Dr Anwar Mohammadi Baneh, Dr Ramin Heshmat, Volume 21, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2016)
Abstract
Background and Aim: Establishment of registry system is an important criterion for health care management system. In this study we extended registry system which included only a few items of personal information. Data concerning epidemiologic issues and important criteria influencing the status of these patients were collected.
Materials and methods: In a cross-sectional study, in cooperation with the specific diseases and organ transplantation management of Ministry of Health and Medical Education, we collected and studied data of the hemodialysis patients in our country. This study included 18,160 patients from 428 dialysis departments. We collected demographic, clinical and laboratory data including demographic characteristics, disease duration, cause of renal disease and recorded serum levels of hemoglobin ,calcium, PTH, ferritin and…. ., during a period of 2 months. Using SPSSv.18 software data were analyzed by t-test.
Results: The mean age of the patients was 67.86 ± 16.7 years. The most common causes of ESRD were diabetes (37.35%) and hypertension (23.9%) respectively. 2.2 percent and 3.06 percent of the patients were positive for HBSAg and HCVAb respectively. Most patients ( 75.7%) had been on dialysis 3 times a week. Hemoglobin levels were less than 10 mg / dl in 42.3 % of the patients . 20.3 % of the patients had calcium levels of less than 8 mg/dl. PTH level was more than 300 mg / dl in 22.6% of the patients.
Conclusion: Poor control of hemoglobin, albumin, ferritin, etc., as predictors of survival in hemodialysis patients can lead to higher mortality and development of cardiovascular disease in these patients. Control of diabetes and high blood pressure can be effective in reducing the incidence of ESRD.
Keywords: Registry, Hemodialysis, Pathogens.
Received: Jan 2, 2016 Accepted: Jun 15, 2016
Amin Akia, Dr Kiomars Amini, Volume 21, Issue 6 (Scientific Journal of Kurdistan University of Medical Sciences 2017)
Abstract
Background and Aim: Staphylococcus aureus can cause a range of diseases including skin disorders, food poisoning and nosocomial infections. Resistance to antibiotics is a major problem and widespread use of antibiotics plays a major role in the emergence of resistant bacteria. The aim of this study was to evaluate the antibiotic resistance and isolation of vancomycin resistance genes in S. aureus strains isolated from clinical samples by multiplex PCR.
Material and methods: 150 clinical samples were collected randomly from ulcers and nasal swabs from medical centers in Tehran from April to July 2015. Biochemical and microbiological tests were performed to identify strains of S. aureus. Antibiotic susceptibility test was conducted by disc diffusion (Kirby-Bauer) method.
Results: The highest resistance rate belonged to clindamycin (83.3 %). Vancomycin and linezolid had the highest sensitivity rate (96.6%). Using multiplex PCR, from 60 human samples only one (1.6%) had VanA and VanB genes, but we did not detect VanC gene in the samples.
Conclusion: The results showed a high prevalence rate of S. aureus with low resistance to vancomycin in the clinical samples.
Key words: Staphylococcus aureus, vancomycin resistance genes, Multiplex PCR.
Received: Jan 3, 2016 Accepted: Jul 13, 2016
Rasool Kawyannejad, Javad Aminisaman, Saeed Mohammadi, Shila Amini, Maryam Mirzaei, Hasanali Karimpour, Volume 25, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2020)
Abstract
Background and Aim: Ventilator-associated pneumonia is a common, serious and costly complication in patients of intensive care units. Oral hygiene is one of the important clinical measures to reduce the amount of pulmonary infection in these patients. Therefore, this study compares the effects of chlorhexidine and orthodontal mouthwashes on prevention of pulmonary infection in patients with endotracheal tracheal intubation under mechanical ventilation in intensive care units.
Materials and Methods: This double-blind clinical trial study was performed on 60 patients undergoing mechanical ventilation in two groups of intervention (n = 30) and control (n = 30) in ICU department of Imam Reza hospital in Kermanshah. In the control group, chlorhexidine (0.2%) mouthwash and in the intervention group, Ortodontol mouthwash was used three times a day. Data on the rate of early and late pneumonia were compared based on CPIS criteria applying t-test and Chi-square.
Results: The incidence of early and delay pneumonia in the intervention group was lower than that of control, but no significant difference was observed (P >0.05). The overall frequency of pulmonary infection in the intervention group was significantly lower than the control group (P = 0.03). The frequency of pneumonia causes was lower in the intervention group, but this decrease was statistically significant in Staphylococcus aureus (P = 0.03).
Conclusion: According to the results of this study, orthodontol mouthwash can be effective in reducing the incidence of pulmonary infection associated with mechanical ventilation.
Rasoul Nasiri Kalmarzi, Saman Amini, Mohsen Shahidi, Sima Touzandeh Jani, Bijam Nouri, Behzad Khalafi, Mr Wesam Kooti, Volume 25, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2020)
Abstract
Background and Aim: Chronic urticaria is a dermal disease with continuous rash, itching or angioedema, papules and inflammatory plaques, which under conditions transformed from the acute phase to the chronic phase, causing depression and anxiety for many years in patients. Chronic urticaria has a significant impact on the quality of life of the patient. The aim of this study was to examined the quality of life and related factors of patients with chronic urticaria in Kurdistan Province.
Materials and Methods: This descriptive study was performed on 228 patients referred to the Allergy Clinic of Besat Hospital in 2016 Census sampling method was used for selecting patient/ Information was gathered by using DQLI questionnaire. Then describe the data by frequency and percentage descriptive and quantitative data were calculated with Chi 2 and Spearman correlation, by using SPSS 15.
Results: The mean age of participants was 34.81 ± 10.55 years. Data analysis showed that there was a significant correlation between quality of life and gender (p <0.001). There was a significant correlation between quality of life and type of disease (p <0.001). However, there was no significant correlation between quality of life and place of residence and education (p> 0.05).
Conclusion: The results showed that chronic urticaria has significant effect on patients' quality life. It seems that these patients were psychologically involved and their level of satisfaction of quality life was reduced.
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