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Showing 2 results for Tavakkoli
Dr Shabnam Mohammadi, Zahra Pakrouh, Mahin Teimouri, Saedeh Haji Pour, Dr Mehdi Karimi, Dr Maryam Mohammadi, Dr Ali Mohammad Pour, Mohammad Hossein Tani Pour, Dr Isaac Hashemi, Dr Mohammad Sookhtanlu, Dr Kamyar Tavakkoli, Fatemeh Mohammadzadeh, Volume 20, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2015)
Abstract
Background and Aim: Methamphetamine causes damage in different body organs. The aim of this study was to investigate the effects of methamphetamine on morphometric changes and biochemical parameters in kidney of adult mice.
Material and Methods: In this experimental study, 56 mice were randomly divided into 7 groups: control and 6 experimental groups. Experimental groups 1, 2 and 3 received methamphetamine 5, 10 and 20 mg/kg respectively for 10 days. Experimental groups 4, 5 and 6 received methamphetamine (5, 10 and 20 mg/kg) respectively for 40 days. After 10 and 40 days, kidneys of the mice of the experimental groups were removed and after tissue processing and imaging, volume of kidney and glomerulus were measured by using cavalieri's principle. In addition blood urea and creatinine levels were measured. Using SPSS software, data were analyzed by means of ANOVA.
Results: Histopatological study showed that methamphetamine increased renal damage in a dose dependent manner. High doses of methamphetamine caused a moderate lobulation, glomerulosclerosis and kidney congestion. There was also a significant difference between the experimental (groups 3 to 6) and control groups in regard to the volumes of kidney and glomerulus (P=0.000).
Significant differences were found in the level of urea in group 6 and creatinine levels in all experimental groups compared to those of control group (P=0.000).
Conclusion: Use of 10 mg/kg of methamphetamine for 40 days led to destructive effects on kidney tissue. Also, it increased serum levels of urea and creatinine.
Keywords: Methamphetamine, Kidney, Mice.
Received: Dec 31, 2014 Accepted: Jun 6, 2015
Dr Shabnam Mohammadi, Nafiseh Lotfian, Parisa Keshavarz, Mahsa Tavakkoli, Dr Maryam Mohammadi, Dr Kamyar Tavakkoli Tabasi, Ali Delshad, Dr Mehdi Karimi, Fatemeh Mohammadzadeh, Volume 21, Issue 5 (Scientific Journal of Kurdistan University of Medical Sciences 2016)
Abstract
Background and Aim: Formaldehyde is an air contaminant which has wide applications in industry and medicine and its destructive effects have been reported on the body systems.
The aim of this study was to investigate the influence of different doses of onion extract on biochemical parameters and histopathology of mice kidneys exposed to formaldehyde.
Material and Methods: 48 adult male mice were randomly divided into six groups. The control group did not receive any injection. The second group received formaldehyde 10 mg/kg for 14 days and the 3rd, 4th ,5th and 6th groups received 5,10,20 and 40 mg/kg/day of onion extract respectively in addition to intraperitoneal doses of formaldehyde. After 14 days, tissue preparation was done and using stereological technique, the sizes of the kidneys and glomeruli were measured. Also, serum urea and creatinine levels were measured. Data were analyzed by SPSS software.
Results: We found significant decrease in the number of renal glomeruli in all of the groups that received onion and also decrease in the volume of glomeruli in the group which had received 10 mg onion/day, compared to those in the control group (P = 0.000). There were significant increase in the level of urea in the groups which had received doses of 10 and 20 mg of onion and in the creatinine levels of the groups which had received daily doses of 5, 20 and 40 mg of onion compared to those in the control group (P = 0.000). Formaldehyde caused renal damage and an increase in the levels of urea and creatinine as well as a decrease in the number and volume of the glomeruli of the kidneys.
Conclusion: Administration of daily doses of 40 mg hydro-alcoholic extract of onion for two weeks caused protective effect on kidney tissue exposed to the formaldehyde.
Key words: Formaldehyde, kidney, Mouse, Onion, Extract, Hydrochloric, Biochemical, Morphometry.
Received: Jun 13, 2015 Accepted: Aug 22, 2016
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