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Background and Aim: Octopamine herbal supplement, recommended for weight loss along with exercise, has antioxidant effects. The purpose of this study was to investigate the simultaneous effect of aerobic training and octopamine on inflammatory factors of brown adipose tissue produced as a result of feeding with deeply heated oil in male rats.
Materials and Methods: 30 Wistar rats were selected and randomly divided into control-poisoning (CP; n=6), training-poisoning (TP; n=6), supplement-poisoning (SP; n= 6), supplement-training-poisoning (STP; n=6), and healthy control (HC; n=6) groups. The rats were fed for four weeks with deeply heated oil. The training program continued for four weeks with an average intensity of 50-65% vo2max for 20 minutes every other day. Octopamine (81 µmol / kg) was given as a supplement 5 days a week for 4 weeks by intraperitoneal injection. Also, we used real-time PCR and Western blot methods to evaluate the expressions of the CCR2 gene and MCP1 protein respectively.
Results: The results showed that after training, MCP1 gene expression was significantly lower in the SP (p=0.001), TP (p=0.027), and STP (p=0.013) groups than that in the CP group. Also, we found that CCR2 gene expression was significantly lower in the SP (p=0.013) compared to that in the CP group, but, the least rate of CCR2 gene expression belonged to the TP and STP groups CP (p≤0.05).
Conclusion: The results showed that aerobic training with octopamine supplementation does not have synergistic effects on CCR2 and MCP-1 gene modifications although aerobic training or octopamine supplementation alone can affect the gene modifications.