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Showing 16 results for MOLOUDI

Abas Ahmadi, Dr Mahnaz Aghaiipoor, Dr Ali Akbar Poorfathollah, Dr Mansour Rezaie, Mahin Nikoo-Ghoftar, Dr Mehrdad Parki , Dr Bahram Nikkhoo, Rohallah Moloudi, Nader Kohan ,
Volume 12, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2007)
Abstract

ABSTRACT Background and Aim: Acute leukemia demonstrating immunophenotypic features of more than 1 cell lineage are referred to as acute leukemia of ambiguous lineage in the World Health Organization classification system. A subtype of acute leukemia is biphenotypic acute leukemia in which the malignant cell population expresses markers of 2 different lineages. This entity has been defined by a scoring system proposed by the European Group for the Immunological Characterization of acute leukemias (EGIL). Cases having a score of greater than 2 for the myeloid and either the B- or T-lymphoid lineages are regarded as biphenotypic acute leukemia in this system. We report morphological, cytochemical, immunophenotypical, and cytogenetic features of a case of acute leukemia referred to our flow cytometry center of Iranian Blood Transfusion Organization (IBTO) in Tehran-Iran. Materials and Methods: Peripheral blood and bone marrow samples of a 52 year-old man with previous diagnosis of AML was submitted to flow cytometry department of IBTO for final diagnosis. Morphology, cytochemistry, and immunophenotypic features of blast cells were studied. Results: Blast cells stained with Wright, were relatively large, with high N/C ratio, and often without any granules. Cytochemical stains (MPO, SBB, NSE, PAS) were negative. Multicolor flow cytometric analysis showed that BM blasts expressed CD19, CD20, CD7, and TdT as lymphoid markers, CD13, CD15, and CD117 as myeloid markers, D34 and HLA-DR as stem cell markers. Cytogenic analysis revealed a normal karyotype. Considering the morphology and CD13 positivity, the patient was diagnosed as a case of MO-AML. The patient was treated with AML chemotherapy regimen. He died 5 months later due to the relapse of the disease. Conclusion: BAL is a rare case of acute leukemia with poor prognosis that can not be diagnosed with morphology and cytochemical stains. Although its clinical and biological significance is not yet determined but correct diagnosis and classification of this disease according to accepted and standard criteria like those of EGIL may help to achieve this aim, improve prognosis, and select a specific therapy. Key words: Biphenotypic acute leukemia, Immunophenotyping, flow cytometry, prognosis
Dr Mohammad Raman Moloudi, Dr Kambiz Hassanzadeh, Shamileh Rouhani, Farid Zandi, Abbas Ahmadi, Pooneh Khalwatian, Dr Amin Rostami, Dr Farshad Sheikh Esmaeili, Dr Esmael Izadpanah,
Volume 19, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2014)
Abstract

Background and Aim: Liver plays important roles in the production of bile, detoxification, and elimination of foreign material and synthesis of plasma proteins. Obstructive cholestasis is one of the liver disorders that can result in increased concentration of oxidants and inflammatory agents in the liver. In traditional medicine, Cichorium intybus has been used as a liver protectant, anti inflamatory and detoxifying agent. The aim of this study was to evaluate the effect of chloroformic extract of Cichorium intybus on liver functional tests and serum level of TNF-α in cholestatic rat model. Materials and Methods: In this experimental study, male Wistar rats were randomly divided into 5 groups (n= 6) including sham operated, control (Bile Duct Ligation (BDL) + vehicle), and 3 groups with BDL + extract treatments (100, 200, 400 mg/kg/day ip). These groups were treated for seven days and on the eighth day, prothrombin time (PT), serum albumin, alanine amino transferase (ALT), aspartate amino transferase (AST), total bilirubin, direct bilirubin, alkaline phosphatase (ALP) and lactate dehydrogenase (LDH), were measured by calorimetric and TNF-α was measured by ELISA methods. The results were analyzed by one way ANOVA. Results: The results of the present study showed that the Cichorium extract (100 mg/kg/day) decreased the serum level of direct bilirubin, AST, ALT, and TNF-α significantly compared to the control group (p <0.05). Furthermore, at the higher dose (200 mg/kg/day) PT, ALP, LDH and AST decreased significantly in comparison to the control group (p <0.05), while the serum albumin level increased significantly in the treated animals (p <0.05). Conclusion: In conclusion, we found that, low doses of chloroformic extract of Cichorium intybus protected the liver against obstructive cholestasis induced -injury. Key words: Cichorium intybus, Obstructive cholestasis, Liver function tests. Received: Apr 29, 2014 Accepted: Jun 28, 2014
Sara Haydari, Dr Kambiz Hassanzadeh, Dr Mohammad Raman Moloudi, Dr Esmael Izadpanah,
Volume 20, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2015)
Abstract

Background and Aim: The exact mechanisms of morphine dependence and withdrawal syndrome remain unclear. Many studies have been performed to find agents with minimal dependency side effects for prevention of withdrawal symptoms. Cinnamomum is a herbal medicine that has been used for respiratory disorders, digestive problems, arthritis, dysmenorrhea, and sore throat. Cinnamomum has been used as an alternative traditional treatment for sedative agents in China and India. This study aimed at investigating the effect of hydroalcoholic extract of cinnamomum on morphine withdrawal symptoms in the male rats. Material and Methods: Adult mal Wistar rats weighting 225 -275 g were randomly selected and divided into 5 groups (8 rats per group). In order to induce dependency, additive doses of morphine were injected subcutaneously for 13 days. On the day 13, 30 minutes after the last dose of morphine, control group received saline ip (1 ml/kg: control) and 3 treatment groups received hydroalcoholic extracts of Cinnamomum (50, 100, 200 mg/kg respectively) intraperitoneally. Thirty minutes later,all groups received naloxone injection (4 mg/kg, ip) and withdrawal symptoms including: jumping, rearing, genital grooming, abdominal writhing, and wet dog shake were recorded for 60 minutes. Results: Our results showed that hydroalcoholic extract of Cinnamomum at doses of 50, 100and 200 mg/kg decreased genital grooming. In addition,all doses of the extract of Cinnamomum decreased the total withdrawal scores signficantly. Conclusion: Hydroalcoholic extract of Cinnamomum was effective in reducing the symptoms of morphine withdrawal. Keywords: Hydroalcoholic Extract of Cinnamomum, Morphine, Withdrawal symptoms. Received: Oct 26, 2014 Accepted: Jan 20, 2015
Abdollah Hassanzadeh, Dr Kaveh Shahvaisi, Dr Kambiz Hassanzadeh, Dr Esmael Izadpanah, Dr Arius Amini, Dr Mohammad Raman Moloudi,
Volume 20, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2015)
Abstract

Background and Aim: Rebamipide is a quinolone derivative drug, which stimulate prostaglandin production and mucus secretion. Despite gastric mucosal protection and ulcer healing effect, it has low solubility and low permeability. An efficient way to increase drug permeability and solubility is to use the polymeric carrier and nanoparticles which effectively increase drug delivery. The aim of this study was to evaluate the effect of rebamipide and encapsulating rebamipide with chitosan capsule on inflammatory mediators in rat experimental colitis. Methods: In this study, 36 male wistar rats were randomly divided into 6 groups (n= 6) including rebamipide-chitosan, rebamipide, chitosan, positive control (hydrocortisone), control (rebamipide vehicle) and sham operated. After 36 h fasting, colitis was induced with 2 ml of acetic acid 4% (except sham operated groups that received saline). After 6 days, animals sacrificed and samples of colon tissues were obtained for MDA, NO and TNF-α levels assessment and determination of MPO and SOD activity. The results were analyzed by one way ANOVA. Results: Encapsulating rebamipide with chitosan capsule not only significantly reduced TNF-α and NO (P <0.03) compared with rebamipide solution, but also diminished other anti-inflammatory mediators and increased antioxidant capacity compared with other groups. Conclusion: Encapsulating rebamipide with chitosan capsule can increase the effectiveness of this drug however more studies are needed to support this hypothesis for clinical use. Key words: Chitosan capsule, ulcerative colitis, Rebamipide, TNF-α and NO. Received: Feb 23, 2015 Accepted: May 11, 2015
Dr Esmael Izadpanah, Fatemeh Nikandam, Dr Mohammad Raman Moloudi, Dr Kambiz Hassanzadeh,
Volume 21, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2016)
Abstract

Background and Aim: There have been reports of beneficial effects of Cinnamomum extract on the respiratory disorders, digestive problems, menstrual pain and inflammatory diseases such as arthritis. The aim of this study was to evaluate the analgesic effects of hydroalcoholic extract of Cinnamomum in rats by using plantar test.

Material and Methods: Wistar male rats were divided into six groups (n=8) randomly. The rats received either hydroalcoholic extract of Cinnamomum (200, 400, mg/kg) alone, or in combination with naloxone or flumazenil intraperitoneally and after 30, 60 and 90 min, the analgesic effects of the extracts was assessed by means of plantar test.

Results: The results showed that injection of hydroalcoholic extract of Cinnamomum (400 mg/kg, ip)  increased significantly the time delay in response to thermal pain inducing effect in pain model of plantar test at 60 (P <0.05) and 90 (P <0.01) min after injection compared to the control group. In addition, we found no significant differences between experimental and control groups in relation to the analgesic effect of 400 mg/kg of Cinnamomum extract, with naloxone or flumazenil and the analgesic effect of Cinnamomum extract was blocked.

Conclusion: Our findings confirmed the analgesic effect of Cinnamomum extract. Considering the effects of flumazenil and naloxone on inhibition of the Cinnamomum analgesia, it seems that opioidergic and GABAergic pathways may be involved in the mechanism of Cinnamomum analgesic effect.

Keyword: Hydroalcoholic extract of Cinnamomum, Analgesic effect, Plantar test.

Received: Apr 3, 2016      Accepted: May 31, 2016


Dr Esmael Izadpanah, Dr Kambiz Hassanzadeh, Dr Vahid Yousefinejad, Dr Kaveh Shahveisi, Nima Fatahi, Dr Mohammad Raman Moloudi,
Volume 21, Issue 5 (Scientific Journal of Kurdistan University of Medical Sciences 2016)
Abstract

Background and Aim: Liver has a major role in protection of human against various toxins and drugs. Cholestasis can be defined as impairment of the bile flow which can lead to increased oxidant stress, hepatocyte damage and finally cirrhosis. Selegiline is commonly used in management of Parkinson's disease. It has neuroprotective, antiapoptic and antioxidant properties. The aim of this study was to evaluate the effect of selegiline on liver cholestasis induced by bile duct ligation (BDL) in the rats.

Material and Methods: 30 male Wistar rats were randomly divided into 5 groups (n= 6) including saline, sham + saline, BDL + saline, BDL + Selegiline (0.15 mg/kg) and Selegiline (0.15 mg/kg). Under general anesthesia and sterile condition, laparatomy was done, and bile duct was ligated. After 14 days, liver function tests, serologic tests and serum TNF-α were performed for all groups after taking blood samples. The results were analyzed by one-way ANOVA.

Results: The results of the this study showed that selegiline significantly increased Alb, AST, ALT and ALP in BDL selegiline group compared to BDL+ saline. Also, when compared to saline group, selegiline significantly increased PT.

Conclusion: Selegiline in cholestatic rat model did not show a protective effect on the liver cells and in some cases exacerbated the symptoms.

Key words: Liver damage, Cholestasis, Selegiline, TNF-alpha.

Received: May 25, 2015      Accepted: Jul 4, 2016


Seyedeh Parastoo Golmohammadi, Dr Bijan Noori, Dr Mohammad Raman Moloudi, Dr Kambiz Hassanzadeh,
Volume 21, Issue 5 (Scientific Journal of Kurdistan University of Medical Sciences 2016)
Abstract

Background and Aim: Adverse drug reactions (ADR) impose costs on the health care system and affect public health. Identification, assessment and prevention of adverse drug reactions are effective factors which can influence social health indices.

Material and Methods: In this cross-sectional study, 133 outpatient and inpatient reports of adverse drug reactions were investigated in Kurdistan Province hospitals between 2013 and 2014. The data included demographic characteristics, type, form, route of administration, type of drug side effects and final outcome. Data were analyzed by chi-square multi response test. P<0.05 was considered significant.

Results: Our results showed that the most common complications were dermatologic and respiratory reactions due to injectable forms of the drugs  and antibiotics were the most common drugs which caused adverse drug reactions. The frequency of adverse drug reactions was higher in the women and these reactions were more common between 40 and 50 years of age. Most cases of adverse drug reactions were reported from Sanandaj and Saqez Cites.

Conclusion: It seems that training programs about ADRs for healthcare workers and avoidance of unnecessary injections can lead to decreased incidence of ADRs.

Key words: Adverse drug reactions, Drug form, Drug administration route.

Received: Jun 15, 2016      Accepted: Jul 26, 2016


Dr Farnoosh Khosrobakhsh, Dr Mohammad Raman Moloudi, Maryam Bigdelo, Arman Rahimi,
Volume 22, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2017)
Abstract

Background and Aim: Cholestasis is characterized by impaired bile flow, which can cause accumulation of bile acids in the liver and development of metabolic disorders, resulting in hepatocellular necrosis and apoptosis. Mitochondria are a critical cellular organelle that produces most of the cellular energy. Mitochondrial morphology varies from an interconnected filamentous network to isolated dots. This processes are called mitochondrial fission and fusion. Disrupted mitochondrial morphology has been observed in cholestatic liver disease. Dynamin related protein 1 is one of the genes involved in mitochondrial fission and plays a role in apoptosis. In this study we investigated Drp1 gene expression in the liver of cholestatic rats.
Materials and Methods: In this experimental study, male Wistar rats (290±25g) were divided into three groups of control (non-operated), sham (operated without common bile duct ligation) and BDL (operated with common bile duct ligation). On the 28th day of BDL, rats were weighed and sacrificed. Biochemical assays for measurement of bilirubin level and liver enzymes, and also dissection of liver tissue for histopathological analysis were performed. Drp1 gene expression was evaluated by semi-quantitative RT-PCR technique.
Results: The results showed that serum levels of total bilirubin and liver enzymes (ALT, AST, ALK) were significantly increased in BDL group compared to those in the control and sham operation groups (P<0.0001 and P<0.001). Histological examination revealed bile ductular hyperplasia, focal liver necrosis and fibrous tissue expansion in BDL group. The result of RT-PCR indicated significant increase of Drp1 gene expression in the liver of the rats in BDL group compared to that in the other groups (P<0.001).
Conclusion: In this study we found that liver cholestasis increased expression of Drp1 gene which led to increased mitochondrial-mediated apoptotic effect with resultant liver cell death.
Keywords: Liver, Cholestasis, Mitochondrial dynamics, Dynamin related protein-1 gene
Received: Jan 30, 2017       Accepted: May 13, 2017
Dr Mohammad Raman Moloudi, Hila Moqbel, Dr Dara Dastan, Katayoon Hasanzadeh, Dr Bijan Noori, Dr Esmael Izadpanah,
Volume 23, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2018)
Abstract

ABSTRACT
Background and Aim: The exact mechanisms of morphine dependence and withdrawal syndrome remain unclear. Many studies have been performed to find agents with low dependency in order to decrease withdrawal symptoms. On the other hand studies have shown the anticonvulsant and sedative effects of Jasminum sambac. The traditional use of this plant has shown its analgesic, anti-depressant, anti-inflammatory, disinfectant, sedative, and anti-spasmodic effects. The aim of this study was to investigate the effect of hydroalcoholic extract of Jasminum sambac on morphine withdrawal symptoms in rats.
Material and Methods: Adult male Wistar rats with weight range of 225 - 275 g were randomly selected and divided into 5 groups. Each group consisted of 6 rats. In order to induce dependency, additive doses of morphine were injected subcutaneously for 13 days. On the 13th day, after the last dose of morphine, intraperitoneal saline injection (1 ml/kg:) was given to the morphine-saline group. We gave intraperitoneal injections of 100, 200, 400 mg/kg of hydroalcoholic extract of Jasminum sambac to the three treatment groups respectively. Thirty minutes later, intraperitoneal injections of naloxone (4 mg/kg) was given to the treatment groups and the withdrawal symptoms including: jumping, rearing, genital grooming, abdominal writhing, wet dog shake and weight loss were recorded for 60 minutes.
Results: Results of this study showed that 100 mg/kg of hydroalcoholic extract of Jasminum sambac significantly reduced the number of jumping and at all doses reduced rearing and genital grooming in the treatment groups compared to those in the morphine-saline group (P <0.01 and P <0.001). In addition, hydroalcoholic extract of Jasminum sambac decreased total withdrawal scores at all used doses.
Conclusion: We found that hydroalcoholic extract of Jasminum sambac was effective in decreasing the symptoms of morphine withdrawal symptoms. This effect is probably attributed to its antioxidant and anti-inflammatory effects.
Keywords: Hydroalcoholic extract of Jasminum sambac, Morphine, Withdrawal symptoms.
 
Received: Aug 7, 2017     Accepted: Nov 1, 2017

Omid Abdollahi, Dr Mohammad Raman Moloudi, Dr Dara Dastan, Katayoon Hassanzadeh, Dr Esmael Izadpanah,
Volume 23, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2018)
Abstract

Background and Aim: There are several reports about analgesic, anti-depressant, anti-inflammatory, antiseptic, sedative, and anti-spasmodic effects of Jasminum sambac. The aim of this study was to investigate the analgesic effects of Jasminum sambac hydroalcoholic extract and determine the role of GABAergic and opioidergic pathways in rat model by a plantar device.
Material and Methods: Wistar male rats were randomly divided into six groups (n=6) including control, the groups treated with hydroalcoholic extract of Jasminum Sambac (100, 200, 400, mg/kg, ip) and the groups which received the most effective dose of the extract in addition to naloxone or flumazenil. The analgesic effect was assessed by plantar device after 30, 60 and 90 min of the injections.
Results: Our results showed that injection of hydroalcoholic extract of Jasminum sambac (200 mg/kg, ip)  increased significantly the time delay in response to thermal pain inducing effect at 30, 60 and 90 (P <0.05) min after injection in the experimental groups compared to that in the control group. While, addition of naloxone, prevented analgesic effect of the extract at all three times (P <0.05). This pattern of reduction of extract analgesic effect was significant only 60 min after concomitant administration of the extract with flumazenil.
Conclusion: The results indicated the analgesic effect of Jasminumsambac extract. Considering the preventive effect of naloxone on the analgesia produced by Jasminum sambac, opioidergic pathway seems to be dominant in the development of Jasminum sambac analgesic effect.
Keyword: Hydroalcoholic extract of Jasminum sambac, Analgesic effect, Opioidergic pathways.
 
Received: Dec 31, 2017     Accepted: May 22, 2018
Dr. Farnoosh Khosrobakhsh, Dr. Mohammad Raman Moloudi, Ms. Khatere Shoja, Ms. Sahel Mohammadi,
Volume 24, Issue 5 (Scientific Journal of Kurdistan University of Medical Sciences 2019)
Abstract

Background and Aim: Cholestasis is characterized by blockade of bile flow from the liver to the intestine, which leads to accumulation of bile acids within liver and plasma; it is associated with metabolic disorders and cause hepatocellular necrosis and apoptosis during cholestatic liver diseases. Mitochondria are critical cellular organelles that produce most of the cellular energy. Mitochondrial morphology varies from an interconnected filamentous network to isolated dots. These processes are called mitochondrial fission and fusion. Disrupted mitochondrial morphology has been observed in cholestatic liver disease. Optic Atrophy 1 (OPA1) is one of the proteins involved in mitochondrial fusion and plays an anti-apoptotic role. The aim of this study was to evaluate the effect of α-lipoic acid (LA) on OPA1 gene expression in pancreas of rat after bile duct ligation (BDL).
Materials and Methods: Thirty-six male Wistar rats were randomly divided into six groups each containing six rats including: control, sham-operated, cholestatic, cholestatic+LA, cirrhotic, and cirrhotic+LA. After 14 days in cholestasis groups and six weeks in cirrhosis groups, serum samples and liver and pancreas tissue samples prepared for total bilirubin assays, histopathological analysis and pancreatic OPA1 gene expression evaluation by Real-time PCR technique. Total bilirubin data and gene expression data were analyzed by one-way ANOVA and Kruskal-Wallis statistical tests. 
Results: The results revealed that serum levels of total Bilirubin were significantly increased in BDL groups as compared with the control and sham operation groups (P<0.0001). Concerning histology, the inflammation Symptoms and tissue necrosis were noted with BDL group. These symptoms were reduced by lipoic acid treatment in the cholestatic group. The result of pancreatic OPA1 gene expression showed the significant increase in cholestatic rats and significant decrease in cirrhotic groups as compared with other groups (P<0.05). In cholestatic group, restoring the expression of OPA1 gene was shown in the presence of lipoic acid.
Conclusion: Changing OPA1 gene expression in obstructive rat suggest the causal role of mitochondrial dynamics in pathogenesis of cholestatic disease. In this study, the effect of lipoic acid in OPA1 mRNA level reflects implications of oxidative stress in signaling pathway of cholestasis.
 

Esmael Izadpanah, Elham Saei, Omid Abdollahi, Abbas Ahmadi, Mohammad Raman Moloudi,
Volume 25, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2020)
Abstract

Background and Aim: There are reports for analgesic, anti-inflammatory, hypnotic, neuroprotective and anti-oxidative effects of safranal. The aim of this study was to investigate the analgesic effects of safranal using acute pain method in rat and determine the role of GABAergic and Opioidergic pathways.
Material and Methods: Wistar male rats were randomly divided into six groups (n=6). Experimental groups including: Control, safranal (1, 2, 4, mg/kg, ip) and the most effective dose of safranal in combination with naloxone or flumazenil receiving groups. The analgesic effect was assessed by plantar apparatus in 30, 60 and 90 min after drugs or vehicle administration.
Results: Our results showed that safranal injection (2 mg/kg, ip) significantly increased the time delay in response to thermal inducing pain effect at 30, 60 and 90 min after injection compared with the control group(P <0.05). While, the administration of flumazenil with the same dose, prevented analgesic effect of safranal at all three times (P <0.05).
Conclusion: Our findings support the analgesic effect of safranal. Considering the effects of flumazenil in preventing the safranal analgesia, it seems that GABAergic system may mediate the analgesic effect of safranal.
Alina Abdollahi, Esmael Izadpanah, Abdollah Hassanzadeh, Shima Khaledyan Shima Khaledyan, Sima Zohrevand, Bahram Ghadermarzi, Dr Mohammad Raman Moloudi,
Volume 25, Issue 5 (Scientific Journal of Kurdistan University of Medical Sciences 2020)
Abstract

Background and Aim: Mood disorders such as anxiety and depression are among the most common psychiatric disorders worldwide. Existing drug therapies have various side effects on the central nervous system. Cinnamomum zeylanicum is a dietary additive and studies have shown that it has antioxidant, anti-inflammatory, analgesic, and neuroprotective effects. Also, in traditional medicine, the sedative properties of cinnamon against anxiety and obsession have been mentioned. Therefore in this study, the effect of Cinnamomum zeylanicum on mood in mice was investigated.
Materials and Methods: In this experimental study, 144 mice weighing 32±4g were divided in two anxiety (Five groups including control, diazepam 2 mg/kg, and three groups of Cinnamomum hydroalcoholic extract 100,200,400 mg/kg, in each test of the elevated plus-maze and Vogel's conflict tests) and depression (Four groups including control and three groups of cinnamon hydroalcoholic extract 100,200,400 mg/kg, in each test of the forced swimming test and tail suspension tests) protocols. Data were analyzed using one-way ANOVA and Tukey’s post-hoc test. P <0.05 were considered significant.
Results: In the anxiety protocol, the results of the elevated plus-maze test showed that the Cinnamomum hydroalcoholic extract at doses of 200 and 400 mg/kg significantly (P <0.01, P <0.001) reduced anxiety. In the depression protocol, the results of the forced swimming test and tail suspension test showed that the Cinnamomum hydroalcoholic extract at a dose of 400 mg/kg was significantly (P <0.05) increased swimming time and mobility compared to the control group.
Conclusion: The results of this study showed that the Cinnamomum hydroalcoholic extract reduced anxiety, increased mobility, and finally mood in mice.


Alina Abdollahi, Dr Esmael Izadpanah, Dr Zakaria Vahabzadeh, Dr Mohammad Raman Moloudi,
Volume 27, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2022)
Abstract

Background and Aim: Non-alcoholic fatty liver disease, characterized by abnormal fat accumulation in the liver, is associated with obesity and insulin resistance. Vaspin is an adipokine secreted by adipose tissue, and its gene expression increases when insulin sensitivity is reduced. In this study, we made a comparison between 3D and 2D cultures in regard to the effects of vaspin on fatty acid metabolism.
Materials and Methods: The steatosis model was induced by oleic and palmitic acid in HepG2 cell line in two- and three-dimensional cultures (collagen gel). Then, the cells were treated with 100 ng/ml vaspin for 24 hours. We used Real time PCR for measurement of the expressions of FABP4, MCAD, FAS and ApoB100 genes.
Results: In two- and three-dimensional cultures, we found significant decrease in the expression of FABP4 and FAS genes. There was no significant difference between the expression of these genes in the two- and three-dimensional cultures. We detected significant increase in the expression of MCAD and ApoB100 genes in 2D and 3D cultures. We did not find any significant difference in the expressions of MCAD and ApoB100 genes between two- and three-dimensional cultures.
Conclusion: Alteration of the expressions of the genes involved in uptake, lipogenesis, oxidation and secretion of fatty acids occurred in the group treated with vaspin compared to the control group in the 2-D and 3-D cultures. But, we did not find any significant difference in the expressions of these genes between the 2-D and 3-D cultures.
Paria Hashemi, Helia Rahmani, Mohammad Raman Moloudi, Zakaria Vahabzadeh, Esmael Izadpanah,
Volume 28, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2023)
Abstract

Background and Aim: Oxidative stress is an important factor in the development of memory and learning disorder which can cause neuronal damage in the hippocampus. Alpha-pinene is a polyphenolic compound from the terpene family that has shown important anti-inflammatory, anti-anxiety, antioxidant and neuroprotective effects in the central nervous system and can affect memory. The aim of the present study was to investigate the effect of alpha-pinene on the improvement of working and spatial memory in rats. 
Materials and Methods: In this study, 24 male rats were randomly divided into 3 groups: control and 2 alpha-pinene groups (5 and 10 mg/kg IP) for 3 weeks. Spatial and working memories were assessed by Morris water maze and Y maze, respectively. Then, malondialdehyde level and total antioxidant capacity in hippocampal tissue were measured. Data were analyzed using one-way analysis of variance and Tukey's post hoc test.
Results: The percentage of alternation in the Y maze increased in the group which had received 10 mg/kg alpha-pinene group compared to those in the control group and the group which had received 5 mg/kg alpha-pinene. The time spent in the target area at the dose of 10 mg/kg of alpha-pinene showed a significant increase compared to that in the control group, but there was no significant difference among the groups in terms of the time to reach the target platform. Alpha-pinene at the dose of 10 mg/kg decreased the level of malondialdehyde in hippocampal tissue compared to the control group, but no significant difference was observed between the groups in terms of total antioxidant capacity.
Conclusion: Alpha-pinene increased spatial and working memory performance in rats. One of the possible mechanisms of memory improvement in the present study could be due to the reduction of malondialdehyde in the hippocampal tissue, as one of the important indicators of oxidative stress in the central nervous system.



 
Paria Hashemi, Mohammad Raman Moloudi, Helia Rahmani, Zakaria Vahabzadeh, Esmael Izadpanah,
Volume 29, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2024)
Abstract

Background and Aim: Huntington's disease is a chronic hereditary disorder that causes cognitive and movement defects in affected individuals by progressive destruction of neurons in the cerebral cortex, striatum and the hippocampus. Studies have shown that increased activity of cyclin-dependent kinase-5 (CDK5) plays an important role in the pathogenesis and occurrence of memory impairment in Huntington's disease. Recently, alpha-pinene has been reported to improve learning and memory performance in Alzheimer's and Parkinson's models. Therefore, the aim of this study was to investigate the effect of alpha-pinene on passive avoidance memory and CDK5 gene expression in Huntington's animal model induced by 3-nitro-propionic acid (3-NP).
Materials and Methods: In this study, 40 male rats were randomly divided into 5 groups: sham, 3-NP (10 mg/kg),and 3 other groups receiving 3-NP (10 mg/kg) + alpha-pinene at doses of 1, 5 and 10 mg/kg (for 3 weeks,via intraperitoneal injection). Passive avoidance memory was assessed through the shuttle box device. Then, the expression level of CDK5 gene was measured by RT-qPCR method in brain cortex and hippocampus. 
Results: 3-NP injection caused memory impairment by decreasing step through latency (STL). Alpha-pinene at all three doses improved passive avoidance memory performance. Also, 3-NP injection caused a significant increase in CDK5 gene expression level in the brain cortex and hippocampus compared to that in the sham group. The groups which received alpha-pinene at doses of 5 and 10 mg/kg in brain cortex and 1 mg/kg in hippocampus showed decreased expression level of this gene compared to the group that received 3-NP. 
Conclusion: The results of this study showed that alpha-pinene improves passive avoidance memory performance probably by reducing the CDK5 gene expression level in Huntington's animal model induced by 3-NP.


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مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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