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:: Volume 17, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2012) ::
SJKU 2012, 17(4): 38-45 Back to browse issues page
Evaluation of the expression of survivin-3b and survivin-3α, the novel splice variants, as diagnostic tumor markers in thyroid cancer
Keihaneh Kiani 1, Mohammad Ali Hosseinpour Feizi Dr 2, Esmail Babaie Dr3, Vahid Montazeri Dr4, Monireh Halimi Dr5
1- Instructor Islamic Azad University, Najafabad Branch
2- Professor Tabriz University , pourfeizi@eastp.ir
3- Assistant Professor University of Tabriz
4- Professor Tabriz University of Medical Science
5- Associate Professor Tabriz University of Medical Science
Abstract:   (10179 Views)
ABSTRACT Background and Aim: Survivin is a new member of inhibitor of apoptosis protein family (IAP) that plays an important role in the regulation of cell cycle and inhibition of apoptosis. Distinct expression of this gene in tumoral cells versus normal cells introduces it as the fourth major transcriptome in cancers. Thyroid carcinoma is the most common endocrine malignancy. Considering the highly heterogeneous nature of tumoral and non-tumoral thyroid nodules from the pathological viewpoint and also in regard to the absence of appropriate molecular markers, extensive efforts have been made to find a specific molecular tumor marker for diagnosis of thyroid tumors. Studies have been demonstrated that the expression pattern of survivin and its splice variants was different in cancerous tissues compared to normal tissues. In this study we evaluated expression of survivin-3b and survivin-3α, the novel survivin splice variants, as diagnostic markers for thyroid cancer. Materials and Method: This was a descriptive study. 77 thyroid specimens including 49 tumoral, 14 non-tumoral and 14 tumor margin samples were collected and expression of survivin-3b ands-3α was investigated by hemi-nested RT-PCR method. Results: Tumoral samples showed the highest expression of survivin-3b and survivin-3α and the lowest expression was detected in the specimens of tumor margins. Conclusion: In this study we demonstrated the expression of survivin-3b and survivin-3α in thyroid tumors for the first time. In conclusion significant expression of survivin-3b and survivin-3α splice variants in tumoral cells shows their roles in thyroid cancer progression and their efficiency as molecular markers for detection and classification of tumoral and non-tumoral thyroid nodules. Key words: Thyroid cancer, Survivin-3b، Survivin-3α, Splice variant, Hemi-nested RT-PCR Received: Aug 15, 2011 Accepted: Aug 30, 2012
Keywords: Thyroid cancer, Survivin-3b, Survivin-3α, Splice variant, Hemi-nested RT-PCR
Full-Text [PDF 259 kb]   (1044 Downloads)    
Type of Study: Research | Subject: General
Received: 2012/12/11
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Volume 17, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2012) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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