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Identification of P fimbriae virulence factor in uropathogenic Escherichia coli by PCR in Shaherkord Hospitals
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| Amir Arsalan Serajian 1, Behnam Zaman Zad Dr2, Parviz Afroogh 3, Mohammad Mehdi Soltan Dallal Dr 4 |
1- Jahad Daneshgahi., Khosestan University
2- Shahrekord University of Medical Sciences
3- Tehran University of Medical Sciences
4- Tehran University of Medical Sciences , soltanirad34@yahoo.com |
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Abstract: (9352 Views) |
| Background and Aim: Urinary tract infection is one of the most common bacterial diseases in human being.The most important etiologic agent is Eschrichia coli & the most important virulence factor in uropathogenic E.coli is P fimberiae. Construction of P fimbriae is coded by pap operon. The importance of P fimbriae is related to the incidence of urinary tract infection, especially infection of the upper tracts. The aim of this study was to identify & determine P fimbriae prevalence in isolated uroparhogenic E.coli.
Material and Methods: A total of 182 isolates of E.coli were evaluated .Bacterial DNA was extracted by boiling method & subsequently DNA samples were examined for pap operon (pap C gene) by use of PCR method. Then PCR products were stained and identified by polyacrylamid gel electrophoresis.
Results: Based on the results of PCR, among 182 isolated strains, 66 samples (36.3%) had pap operon(pap C gene).
Conclusion: The results indicated that prevalence of pap operon in isolated strains was within the global range. Pyelonephritis was more common than cystitis & asympthomatic bacteriuria. |
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| Keywords: Urinary tract infection, Pap operon, Pap C gene, P fimbriae. |
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Full-Text [PDF 819 kb]
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Type of Study: Research |
Subject:
General
Received: 2012/07/2
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