1- Jahad Daneshgahi., Khosestan University 2- Shahrekord University of Medical Sciences 3- Tehran University of Medical Sciences 4- Tehran University of Medical Sciences , soltanirad34@yahoo.com
Abstract: (12557 Views)
Background and Aim: Urinary tract infection is one of the most common bacterial diseases in human being.The most important etiologic agent is Eschrichia coli & the most important virulence factor in uropathogenic E.coli is P fimberiae. Construction of P fimbriae is coded by pap operon. The importance of P fimbriae is related to the incidence of urinary tract infection, especially infection of the upper tracts. The aim of this study was to identify & determine P fimbriae prevalence in isolated uroparhogenic E.coli.
Material and Methods: A total of 182 isolates of E.coli were evaluated .Bacterial DNA was extracted by boiling method & subsequently DNA samples were examined for pap operon (pap C gene) by use of PCR method. Then PCR products were stained and identified by polyacrylamid gel electrophoresis.
Results: Based on the results of PCR, among 182 isolated strains, 66 samples (36.3%) had pap operon(pap C gene).
Conclusion: The results indicated that prevalence of pap operon in isolated strains was within the global range. Pyelonephritis was more common than cystitis & asympthomatic bacteriuria.
Serajian A A, Zaman Zad B, Afroogh P, Soltan Dallal M M. Identification of P fimbriae virulence factor in uropathogenic Escherichia coli by PCR in Shaherkord Hospitals. SJKU 2012; 17 (2) :36-43 URL: http://sjku.muk.ac.ir/article-1-798-en.html