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:: Volume 17, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2012) ::
SJKU 2012, 17(2): 36-43 Back to browse issues page
Identification of P fimbriae virulence factor in uropathogenic Escherichia coli by PCR in Shaherkord Hospitals
Amir Arsalan Serajian 1, Behnam Zaman Zad Dr2, Parviz Afroogh 3, Mohammad Mehdi Soltan Dallal Dr * 4
1- Jahad Daneshgahi., Khosestan University
2- Associate Professor Shahrekord University of Medical Sciences
3- Tehran University of Medical Sciences
4- Professor Tehran University of Medical Sciences , soltanirad34@yahoo.com
Abstract:   (8923 Views)
Background and Aim: Urinary tract infection is one of the most common bacterial diseases in human being.The most important etiologic agent is Eschrichia coli & the most important virulence factor in uropathogenic E.coli is P fimberiae. Construction of P fimbriae is coded by pap operon. The importance of P fimbriae is related to the incidence of urinary tract infection, especially infection of the upper tracts. The aim of this study was to identify & determine P fimbriae prevalence in isolated uroparhogenic E.coli. Material and Methods: A total of 182 isolates of E.coli were evaluated .Bacterial DNA was extracted by boiling method & subsequently DNA samples were examined for pap operon (pap C gene) by use of PCR method. Then PCR products were stained and identified by polyacrylamid gel electrophoresis. Results: Based on the results of PCR, among 182 isolated strains, 66 samples (36.3%) had pap operon(pap C gene). Conclusion: The results indicated that prevalence of pap operon in isolated strains was within the global range. Pyelonephritis was more common than cystitis & asympthomatic bacteriuria.
Keywords: Urinary tract infection, Pap operon, Pap C gene, P fimbriae.
Full-Text [PDF 819 kb]   (1110 Downloads)    
Type of Study: Research | Subject: General
Received: 2012/07/2
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Volume 17, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2012) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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