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Evaluation of DPT Metastasis Suppressor Gene Expression in Human Gastric Cancer
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Sara Rafi Taheri1    , Arezoo Farhadi1 , Zahra Shahsavar Haghighi1 , Ebrahim Hazrati2 , Foad Heidari Mohammad2 , Peyman Aslani2 , Mojgan Mohammadimehr2 , Reza Heidari2 , Javad Behroozi 3, Ali Zarei2 |
1- Varamin- Pishva Branch, Islamic Azad University
2- AJA University of Medical Sciences
3- AJA University of Medical Sciences , jvdbehroozi@gmail.com |
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Abstract: (107 Views) |
ABSTRACT
Background and Aim: Gastric carcinoma (GC) is one of the most common human cancers. Many genes have been analyzed in an attempt to better understand gastric carcinogenesis, however, the underlying mechanism of gastric carcinogenesis is still poorly understood. Dermatopontin (DPT) is an extracellular matrix protein, which regulates multiple physiological processes. The present study aimed to evaluate DPT gene expression in GC.
Materials and Methods: Biopsies of 50 surgically-excised GC tissue specimens and corresponding adjacent normal tissues were examined by real-time PCR. We then performed a bioinformatics analysis to determine DPT gene expression in two different cohorts of GC patients. To determine the effect of DPT expression levels on survival outcomes, a Kaplan–Meier analysis were performed. For a comprehensive analysis, DPT gene expression was evaluated in 16 different cancers.
Results: RT-PCR demonstrated that DPT gene expression was decreased in gastric cancer tissues, when compared with normal stomach tissues. Mean DPT expression value was significantly lower in different stages of GC. Further, survival analysis revealed that the mRNA expression of DPT is positively correlated with overall survival of GC patients. A relationship was found between DPT expression and primary size of tumor. Pan-cancer analysis across 16 tumor types showed that DPT expression is lower in tumor tissue than adjacent normal tissue.
Conclusion: These findings suggest that the decrement of DPT gene expression in gastric tissue may play an important role in gastric carcinogenesis.
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| Keywords: Gastric cancer, DPT, Gene expression, Metastasis |
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Type of Study: Original Research |
Subject:
Molecular Medicine and Genetics
Received: 2022/11/28 | Accepted: 2023/07/11
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