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:: Volume 13, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2008) ::
SJKU 2008, 13(1): 51-58 Back to browse issues page
Detection of -thalassemia mutations in Kurd patients of Kurdistan and West Azerbaijan provinces
SH Khorshidi , M Haghi , M.A Hosseinpour Feizi Dr , M Aminbakhsh Dr, A Hosseinpour Feizi Dr, N Pouladi
Abstract:   (11705 Views)

  ABSTRACT

  Background and Aim: β -thalassemia is the most common autosomal recessive disorder. More than 200 different mutations in the β-globin gene have been detected which can lead to decreased or absent β -globin chain synthesis . Since the Iranian population is a mixture of different ethnic groups, it is necessary to determine the frequency and distribution of these mutations in the different ethnic groups of our country. Therefore, in this study we determined the spectrum and the frequency of β-thalassemia mutations in the patients with β-thalassemia major in the Kurd population of Kurdistan and West Azerbaijan provinces of Iran.

  Material and Methods: To detect mutations, extracted DNA of 110 chromosomes from 55 unrelated patients, were studied by PCR-ARMS (Polymerase Chain Reaction-Amplification Refractory Mutation System), SSCP (Single Strand Conformation Polymorphism) and direct sequencing methods.

  Results: The results of this study showed that IVS-II-1 ¨ (G→A) was the most common mutation with a frequency of 31% FSC * 8/9(+G) with a frequency of 19% was the second most prevalent mutation among all chromosomes. Other mutations were IVS-I-1(G→A), FSC8 (-AA), IVS-I-110(G→A), FSC36/37(-T), IVS-I-5(G→C), IVS-I-128(T→G), FSC44 (-C), FSC 5(-CT) and +22UTR · (G→A). These mutations comprised 79 % of β -thalassemia mutations in this region and 21% of the mutations still remains to be explored.

  Conclusion: The results of this study showed that, there are similarities and differences between this region and other parts of Iran and also neighboring countries. Therefore, determination of β-thalassemia mutations in this region seems to be necessary and beneficial for designing prenatal diagnosis programs.



  ¨ . Intervening Sequence-II-nucleotide 1

  * . Frame Shift Codon

  · . Untranslated Region

Keywords: Key words: -thalassemia, Mutation, Kurd
Full-Text [PDF 132 kb]   (1096 Downloads)    
Type of Study: Research | Subject: General
Received: 2009/04/12
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Khorshidi S, Haghi M, Hosseinpour Feizi M, Aminbakhsh M, Hosseinpour Feizi A, Pouladi N. Detection of -thalassemia mutations in Kurd patients of Kurdistan and West Azerbaijan provinces. SJKU. 2008; 13 (1) :51-58
URL: http://sjku.muk.ac.ir/article-1-74-en.html


Volume 13, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2008) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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