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Molecular Identification of Mycoplasma Hominis in Vaginal Sample of Women Referred to the Infertility Center of Fatemieh Hospital in Hamadan
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Hadi Hossainpour1    , Mohammad Yousef Alikhani2 , Rasoul Yousefi Mashouf3 , Manouchehr Karami4 , Soghra Rabiee5 |
1- MSc student of Microbiology, School of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran
2- Professor of bacteriology, School of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran
3- Professor of Microbiology, School of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran, , yousefimash@yahoo.com
4- Professor of Epidemiology, Hamadan University of Medical Sciences, Hamadan, Iran
5- Professor of Obstetrics and gynecology, Hamadan University of Medical Sciences, Hamadan, Iran |
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Abstract: (1755 Views) |
Background and Aim: Mycoplasma hominis is one of the smallest bacteria isolated from a natural source and is sometime as pathologic flora in plants, animals, and humans. Some of these bacteria are normal flora of the respiratory and genital systems. The aim of the study was molecular identification of M. hominis in a vaginal sample of women referred to the infertility center of Fatemieh Hospital in Hamadan.
Materials and Methods: In this Cross-sectional study, vaginal swab samples were collected from symptomatic females and M. hominis was identified by PCR and Real-Time PCR. DNA was extracted using an extraction kit. M. hominis strains were identified using 16 S rRNA gene. Finally, Statistical analysis was performed using SPSS 21 software.
Results: A total of 234 vaginal samples were studied in women with a mean age of 39.8years. The results showed that M. hominis was identified by PCR (13%) and Real-time PCR (15%) methods, respectively. There was a significant relationship between PCR and Real-Time PCR methods (P≤0.05).
Conclusions: Real-time PCR assay was successfully used for rapid and accurate diagnosis of M.hominis. This method has significant potential for rapid, accurate, and is highly sensitive molecular detection of M. hominis compared to the PCR technic.
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| Keywords: Mycoplasma hominis, Infertile women, PCR, Real-Time PCR |
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Full-Text [PDF 497 kb]
(762 Downloads)
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Type of Study: Original Research |
Subject:
Microbiology
Received: 2021/08/1 | Accepted: 2021/11/14 | Published: 2022/09/11
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