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:: Volume 16, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2012) ::
SJKU 2012, 16(4): 83-91 Back to browse issues page
Evaluation of molecular mechanisms resistance to macrolide by S. pneumoniae strains isolated from Nemazee and Shahid Faghihi Hospitals in Shiraz
Mohammad Kargar Dr 1, Maryam Baghernejad 2, Sadegh Ghorbani-Dalini 2, Zahra Hashemizadeh 3
1- Associate Professor Islamic Azad University, Jahrom Branch, , mkargar@jia.ac.ir
2- Islamic Azad University, Jahrom Branch,
3- Shiraz University of Medical Sciences
Abstract:   (16333 Views)
ABSTRACT Background and Aim: Macrolide Streptococcus pneumoniae is generally mediated by two mechanisms: 23SrRNA methylation and efflux. The aim of this study was to assess the molecular mechanism of resistance in S.pneumoniae strains isolated from intensive care units in Shiraz. Materials and Methods: This was a cross – sectional study. 50 strains of S.pneumoniae were obtained from the patients admitted to intensive care units (ICU) of Nemazee and Shahid-Faghihi Hospitals, from 2010 to 2011. Suspected colonies were identified by phenotypical and biochemical tests. Organisms were confirmed to be Streptococcus pneumoniae on the basis of the presence of lytA gene by PCR method. Antibiotic resistance was evaluated according to Standard Clinical and Laboratory Standards Institute (CLSI). Macrolide resistance genes were identified by use of ermB and mefA specific primers. Using SPSS software, statistical analysis was performed by means of chi- square test. Results: Resistance to erythromycin, clarithromycin and azithromycin levofloxacin were 18%, 48% and 44% respectively. In this study, 25 strains (50%) had mefA and 21 (42%) had ermB genes. Erythromycin resistance had significant relationships with mefA and ermB genes. Conclusion: The results of our study showed that the predominant mechanism of macrolide resistance was due to mefA gene in this area. Increased macrolide resistance calls for attention to the pattern of resistance in the therapeutic regimens. Key words: S. pneumoniae, Macrolide Resistance, mefA, ermB Received: Apr 10, 2011 Accepted: Oct 20, 2011 Conflict of interest: Nill
Keywords: S. pneumoniae, Macrolide Resistance, mefA, ermB
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Type of Study: Research | Subject: General
Received: 2011/12/4
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Volume 16, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2012) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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