[Home ] [Archive]   [ فارسی ]  
:: Volume 16, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2011) ::
SJKU 2011, 16(3): 39-47 Back to browse issues page
The role of typical Enteroaggregative Escherichia coli (tEAEC) strains in diarrhea among Children
Mohammad Mehdi Aslani Dr1, Ali Zavari 2, Mohammad Yousef Alikhani Dr 3
1- Associate Professor Iran’s Pasture Institute
2- Hamadan University of Medical Sciences
3- Associate Professor Hamadan University of Medical Sciences , alikhani43@yahoo.com
Abstract:   (7002 Views)
ABSTRACT Background and Aim: Enteroaggregative Escherichia coli (EAEC) strains have been implicated as causative agents of acute, chronic and persistent diarrhea in children. EAEC have special aggregative adherence pattern for HEp-2 or HeLa cells. PCR methods based on the presence of essential virulence factors would facilitate the diagnosis of EAEC strains. The aim of this study was to assess the usefulness of the PCR assay targeting the pCVD432gene for the detection of EAEC strains and the presence of aggR, agg, aafA, aap and astA genes which can be used for the differentiation of typical and atypical EAEC strains. Materials and Methods: One hundred and forty faecal samples were collected from children of less than 12 years of age with diarrhea at the Besat Hospital, in Hamadan, between July 2007 and May 2008. The faecal samples were plated onto MacConkey agar and incubated at 37 oC for 18 hours. E.coli colonies were identified by biochemical tests. EAEC strains were identified by ues of pCVD432 primer which is specific for EAEC strains. Then their virulence factors were evaluated by use of specific primers of aggR, aggA, aafA, aap and astA genes. Results: Strains of E. coli harboring the pCVD432 gene were found in the faecal samples from 15 (10.8%) patients. The aggR gene was present in 11(73.3) strains. The aggA gene was found in 3 isolates and 4 strains had the aafA gene. The aap and astA genes were found in 9 and 7 strains, respectively. Several different combinations of the genetic markers were found among the EAEC strains. The most prevalent combinations were aggR/aap, found in 8 (53.3%) and aggR/aafA detected in 4(26.7%) strains. Conclusions: The results of this study showed that EAEC strains were one of the most prevalent enteric pathogens in children in this region. The pCVD432 gene PCR assay is the most common method for detection of EAEC strains. Typical EAEC strains with aggR gene had a high prevalence in this region and considering the pattern of virulence factors, they were heterogenous. Key words: EAEC, PCR, children Conflict of Interest: Nill Received: Jan 31, 2011 Accepted: Sep 6, 2011
Keywords: EAEC, PCR, children
Full-Text [PDF 1523 kb]   (1423 Downloads)    
Type of Study: Research | Subject: General
Received: 2011/10/6
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print



Volume 16, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2011) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.09 seconds with 30 queries by YEKTAWEB 3752