[Home ] [Archive]   [ فارسی ]  
:: Volume 16, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2011) ::
SJKU 2011, 16(3): 9-19 Back to browse issues page
The effect of guidance channel on sciatic nerve repair in adult rats
Hamdollah Delaviz Dr1, Abolfazl Faghihi Dr2, Alireza Azizzadeh Delshad Dr3, Mohammad Hadi Bahadori Dr4, Jamshid Mohammadi Dr1, Amrollah Roozbehi Dr * 5
1- Assistant Professor Yasuj University of Medical Sciences
2- Associate Professor Tehran University of Medical Sciences
3- Associate Professor Shahed University of Medical Sciences
4- Associate Professor Guillan University of Medical Sciences
5- Assistant Professor Yasuj University of Medical Sciences , aroozbehi@yahoo.com
Abstract:   (7726 Views)
ABSTRACT Background and Aim: Peripheral nerve lesions are a challenge for neurosurgeons and different surgical repairing methods are applied for the treatment of this problem. This study was conducted to evaluate the poled polyvinelidene fluoride (PVDF) tube filled with nerve growth factor (NGF) and collagen gel as a substitute for nerve autograft. Material and Methods: In this experimental study the left sciatic nerve was manipulated in 50 male Wistar rats and then the animals were divided randomly into five groups. In the epineural group the injured nerves were repaired by end to end suture. In the rats with autograft a 10 mm piece of sciatic nerve was rotated through 180° and sutured in the nerve gap. In the nerve guidance channel group (NGC), polarized piezoelectric PVDF tube containing NGF and collagen gel was replaced in the gap and in the axotomy group two nerve ends were hidden among muscles. The left sciatic nerve was exposed but not transected in the sham group. After two months L4-L6 segment neurons of spinal cord were studied histologically and by immunohistochemical and axonal DiI tracing. The collected data were analyzed by one way ANOVA, LSD and paired t- test. Results: The mean number of Bax positive cells and labeled motor neurons increased significantly in axotomy and sham group respectively, compared to the other groups (P<0.05). Also, the mean number of labeled motor neurons increased significantly in epinural group in comparison to the autograft and the NGC groups (P<0.05). There was no significant difference in the mean number of the labeled neurons between the autograft and nerve guidance channel groups. The mean number of motor neurons in the left side showed a significant decrease in comparison to that of the right side (p<0.01). Conclusion: The PVDF tube together with other therapies provided a favorable environment for nerve regeneration and could be used as a substitute for autograft in nerve injuries. Key words: Guidance channel, NGF, PVDF, Sciatic nerve repair, Spinal motor neurons, Tracing. Conflict of Interest: Nill Received: Nov 19, 2010 Accepted: Jun 14, 2011
Keywords: Guidance channel, NGF, PVDF, Sciatic nerve repair, Spinal motor neurons, Tracing.
Full-Text [PDF 1698 kb]   (1570 Downloads)    
Type of Study: Research | Subject: General
Received: 2011/10/6
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >


XML   Persian Abstract   Print



Volume 16, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2011) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.08 seconds with 30 queries by YEKTAWEB 3705