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:: Volume 16, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2011) ::
SJKU 2011, 16(2): 36-44 Back to browse issues page
Expression of recombinant Hc domain of Clostridium botulinum neurotoxin A in E. coli and its purification as a vaccine candidate against botulism
Seyed Safa Ali Fatemi Dr1, Khairollah Yari 2, Mahmood Tavallaei Dr3, Danial Kahrizi Dr4
1- Assistant Professor National Institute of Genetic Engineering and
2- Instructor Kermanshah University of Medical , khirollah.yari@yahoo.com
3- Associate Professor Baqiyatallah Medical Sciences University
4- Assistant Professor Razi University
Abstract:  
ABSTRACT Background and Aim: Botulism is a lethal disease which is caused by the one of the neurotoxins of the 7 types of Clostridium botulinum. The carboxylic domain of the heavy chain of Clostridium botulinum type A neurotoxin (BoNT/A-Hc), is highly capable of activating immune system and is used for production of a vaccine against botulism. The aim of this study was to express and produce soluble form of BoNT/A-Hc in recombinant E. coli. Materials and Methods: Hc part of BoNT/A was subcloned in pET28a vector and clone was expressed in E. coli BL21 (DE3). Then the best recombinant clones were selected based on three main factors: expression, growth and plasmid stability. Then protein expression was evaluated in LB and M9 media and the protein was purified by resin column chromatography. Results: In flask culture, under optimal conditions of bacterial culture yielded 52mg of BoNT/A-Hc soluble protein from each liter of culture medium. Conclusions: According to the results of this study, selection of suitable strains on the basis of important growth indices of the bacteria, expression of recombinant protein and plasmid stability can lead to in increased efficiency of the recombinant protein production. Key Words: Protein Expresion, E. coli, Clostridium botulinum, Botulism Conflict of Interest: Nill Received: Feb 15, 2011 Accepted: May 18, 2011
Keywords: Protein Expresion, E. coli, Clostridium botulinum, Botulism
Full-Text [PDF 4588 kb]      
Type of Study: Research | Subject: General
Received: 2011/08/2
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Volume 16, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2011) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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