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:: Volume 16, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2011) ::
SJKU 2011, 16(1): 56-63 Back to browse issues page
Optimization of Loop-Mediated Isothermal Amplification (LAMP) method for detection of herpes simplex virus type 1 and 2
Asmaa Bahrami 1, Mohammad Hasan Shahhosseiny Dr 2, Kayhan Azadmanesh Dr3, Zahra Noormohamadi Dr4, Elham Moslemi Dr5, Farzaneh Jadali Dr6
1- Islamic Azad University, Science & Research Branch, Tehran
2- Associate Professor Islamic Azad University, Shahr-e- Qods Branch , shahhosseiny@yahoo.com
3- Assistant Professor Pasteur Institute of Iran
4- Assistant Professor Islamic Azad University, Science & Research Branch
5- Assistant Professor Islamic Azad University, East Tehran Branch
6- Associate Professor Pathology Dept., Mofid Hospital of Tehran
Abstract:   (12256 Views)
ABSTRACT Background and Aim: Type 1 and type 2 herpes simplex (HSV) virus cause infection of central nervous system (encephalitis) in human. The molecular techniques are the best methods for detection of HSV. In this study we evaluated the novel molecular technique of LAMP for detection of HSV-1 and HSV-2. Material and Methods: In this experimental study 184 cerebrospinal fluid (CSF) samples were collected from Mofid Hospital. DNA of every sample was extracted by use of Sinagen DNP Kit. Based on the HSV DNA polymerase gene, a set of 6 primers were designed and sensitivity and specificity of this method were determined. By adding SYBER Green, LAMP product was identified. The results of LAMP method were compared to those of PCR by chi-square test. Results: Sensitivity of LAMP method determined to be 5 copies/ tube and sensitivity of PCR method determined to be 50 copies/ tube. Both LAMP and PCR methods showed 100% specificity for detection of HSV type 1 and type2. Among 184 samples, 60 samples were positive by LAMP but 45 samples were positive by PCR method. Sensitivity of LAMP was 10 times higher than that of PCR. Comparison of the results of the two methods by means of chi-square test showed a significant difference (p‹0.05). Conclusion: LAMP method had high sensitivity and specificity for detection of type 1 and type 2 HSV in CSF samples. Key word: HSV, LAMP, CSF Conflict of Interest: Nill Received: Nov 22, 2010 Accepted: Jan 14, 2011
Keywords: HSV, LAMP, CSF
Full-Text [PDF 1671 kb]   (1377 Downloads)    
Type of Study: Research | Subject: General
Received: 2011/05/10
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Volume 16, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2011) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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