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:: Volume 22, Issue 5 (Scientific Journal of Kurdistan University of Medical Sciences 2017) ::
SJKU 2017, 22(5): 55-65 Back to browse issues page
Simulated microgravity effects on cell cycle in adipose derived stem cells
Farid Ebnerasuly 1, Zahra Hajebrahimi Dr 2, Seyed Mehdi Tabaie Dr3, Mojtaba Darbouy Dr4
1- Department of Biology, Fars Science and Research Branch , Islamic Azad University, Marvdasht, Iran.
2- Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, Iran , hajebrahimi@ari.ac.ir
3- Medical Laser Research Center, Iranian Academic Center for Education, Culture and Research (ACECR), Tehran, Iran.
4- Department of Biology, Fars Science and Research Branch, Islamic Azad University, Marvdasht, Iran.
Abstract:   (862 Views)
Background and Aim: In recent years, biophysical forces especially microgravity increasingly has been used for cell manipulation, in particular cell differentiation and tissue engineering.  Microgravity, as a mechanical factor, has been demonstrated to stimulate cell proliferation and differentiation. In this study, we investigated the impact of simulated microgravity condition generated by one-axis clinostat, on cell cycle and apoptosis rate of adipose derived stem cells (ADSCs).
Material and Methods: After isolation and characterization of human ADSCs, they were exposed to simulated microgravity condition generated by one-axis clinostat, for 3 days. After 3 days of clinorotation, control and microgravity samples were stained with PI, then, we evaluated cell cycle and apoptosis rate.
Results: Isolated cells expressed mesenchymal markers and were able to differentiate into adipocytes and osteocytes. Our results showed that 3 days of simulated microgravity induced by one-axis clinostat had no statistically significant effects on the cell cycle and apoptosis rate.
Conclusion: Previous studies have shown that simulated microgravity induced by 3-axis clinostat or RPM altered cell cycle and increased apoptosis rate. Based on our data, it seems that shear stress generated by one-axis clinostat is much less than that generated by three-axis clinostat. Therefore, we recommend use of one-axis clinostat for evaluation of cell differentiation and cell therapy under microgravity conditions for the future studies. 
Keywords: Adipose derived stem cells, Simulated microgravity, One-axis clinostat.
 
Received: Feb 4, 2017     Accepted: Jul 8, 2017
Keywords: Adipose derived stem cells, Simulated microgravity, One-axis clinostat.
Full-Text [PDF 273 kb]   (301 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/11/20 | Accepted: 2017/11/20 | Published: 2017/11/20
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Ebnerasuly F, Hajebrahimi Z, Tabaie S M, Darbouy M. Simulated microgravity effects on cell cycle in adipose derived stem cells. SJKU. 2017; 22 (5) :55-65
URL: http://sjku.muk.ac.ir/article-1-3555-en.html


Volume 22, Issue 5 (Scientific Journal of Kurdistan University of Medical Sciences 2017) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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