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:: Volume 12, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2007) ::
SJKU 2007, 12(3): 8-22 Back to browse issues page
Study of the bone produced from canine mesenchymal stem cell differentiation
Mohammad Reza Baghban Islami Nejad Dr 1, Aghbibi Nik Mahzar , Abas Piriaiee
1- Assistant Professor , bagesla@yahoo.com
Abstract:  
Background and Aim: The aim of the present study was to isolate mesenchymal stem cells (MSCs) from canine bone marrow, differentiate them into bone and examine the structure of the produced bone that was not reported before. Materials and Methods: MSCs from canine bone marrow were extracted, expanded and cultured in an osteogenic medium which differentiated into bone tissue on the surface of the cover slips. At the end of the differentiation period, the bone prepared was examined with light and electron microscopes. In this study, the isolated cells were also differentiated into adipocytic cell lineage. Results: Cultivated mesenchymal stem cells formed several nodule-like structures during differentiation period. According to the light microscopic images, these nodules were composed of several layers of cells with an abundant matrix among the cells. Transmission electron microscopic study indicated that the cells were mainly of spindle-shape morphologly similar to osteocytic cells, and the matrix consisted of collagen fibers with sharp striation organized in bundles perpendicular to each other. Conclusion: It seems that the bone produced from in vitro differentiation of the MSCs, histologically is similar to that of laminar bone particularly in terms of the collagen fiber arrangement. Key words: Canine Mesenchymal Stem Cells, Bone Differentiation, Bone ultra-structure
Keywords: Canine Mesenchymal Stem Cells, Bone Differentiation, Bone ultra-structure
Full-Text [PDF 24 kb]      
Type of Study: Research | Subject: General
Received: 2009/04/8
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Volume 12, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2007) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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