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:: Volume 12, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2007) ::
SJKU 2007, 12(3): 85-91 Back to browse issues page
Report of a case of Biphenotypic Acute Leukemia according to EGIL criteria
Abas Ahmadi * 1, Mahnaz Aghaiipoor Dr2, Ali Akbar Poorfathollah Dr3, Mansour Rezaie Dr, Mahin Nikoo-ghoftar , Mehrdad Parki Dr2, Bahram Nikkhoo Dr2, Rohallah Moloudi 4, Nader Kohan
1- , abbasahmady@yahoo.com
2- Assistant Professor
3- Associate Professor
4- Instructor
Abstract:   (14102 Views)
ABSTRACT Background and Aim: Acute leukemia demonstrating immunophenotypic features of more than 1 cell lineage are referred to as acute leukemia of ambiguous lineage in the World Health Organization classification system. A subtype of acute leukemia is biphenotypic acute leukemia in which the malignant cell population expresses markers of 2 different lineages. This entity has been defined by a scoring system proposed by the European Group for the Immunological Characterization of acute leukemias (EGIL). Cases having a score of greater than 2 for the myeloid and either the B- or T-lymphoid lineages are regarded as biphenotypic acute leukemia in this system. We report morphological, cytochemical, immunophenotypical, and cytogenetic features of a case of acute leukemia referred to our flow cytometry center of Iranian Blood Transfusion Organization (IBTO) in Tehran-Iran. Materials and Methods: Peripheral blood and bone marrow samples of a 52 year-old man with previous diagnosis of AML was submitted to flow cytometry department of IBTO for final diagnosis. Morphology, cytochemistry, and immunophenotypic features of blast cells were studied. Results: Blast cells stained with Wright, were relatively large, with high N/C ratio, and often without any granules. Cytochemical stains (MPO, SBB, NSE, PAS) were negative. Multicolor flow cytometric analysis showed that BM blasts expressed CD19, CD20, CD7, and TdT as lymphoid markers, CD13, CD15, and CD117 as myeloid markers, D34 and HLA-DR as stem cell markers. Cytogenic analysis revealed a normal karyotype. Considering the morphology and CD13 positivity, the patient was diagnosed as a case of MO-AML. The patient was treated with AML chemotherapy regimen. He died 5 months later due to the relapse of the disease. Conclusion: BAL is a rare case of acute leukemia with poor prognosis that can not be diagnosed with morphology and cytochemical stains. Although its clinical and biological significance is not yet determined but correct diagnosis and classification of this disease according to accepted and standard criteria like those of EGIL may help to achieve this aim, improve prognosis, and select a specific therapy. Key words: Biphenotypic acute leukemia, Immunophenotyping, flow cytometry, prognosis
Keywords: Biphenotypic acute leukemia, Immunophenotyping, flow cytometry, prognosis
Full-Text [DOC 247 kb]   (1748 Downloads)    
Type of Study: Research | Subject: General
Received: 2009/10/27 | Accepted: 2015/01/10 | Published: 2015/01/10
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Volume 12, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2007) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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