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:: Volume 19, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2014) ::
SJKU 2014, 19(3): 130-140 Back to browse issues page
Evaluation of the two variants of p63 (d4TAp63& ΔNp73L) expression as molecular markers in the diagnosis and prognosis of breast cancer
Maryam Moradi Nasab 1, Mohammad Ali Hossein Pour Feizi Dr 2, Reyhaneh Ravanbakhsh Gavgani 1, Naser Pouladi Dr3, Aeeda Sedaie bonab 1, Parvin Azarfam 1, Vahid Montazeri Dr4, Ashraf Fakhrjoo Dr4
1- Tabriz University
2- Professor Tabriz University , pourfeizi@eastp.ir
3- Assistant Professor Azarbaijan Shahid Madani University
4- Professor Tabriz University of Medical Sciences
Abstract:   (3255 Views)
Background and Aim: The TP63 gene, as the oldest homologue of TP53, encodes two main N-terminal variants by different promoters: the trans-activating variant, TAp63 with tumor suppressor activity and the amino terminal truncated variant, ΔNp63 with oncogenic activity. As the result of the deletion of exon 4 in each of the variants, two further N-terminal variants have been reported for p63 in the last decade: d4TAp63 and ΔNp73L, but the exact function of these variants have not been determined in normal and tumoral cells. In this study we evaluated the expression pattern of p63 variants and usefulness of d4TAp63 and ΔNp73L as potential diagnostic molecular markers in breast cancer. Materials and Methods: In this study 30 tumoral and 20 non tumoral samples of marginal tissues were studied by use of Semi-quantitative Reverse Transcriptase-nested PCR (RT-nPCR) method. SPSS16 software was used for data analysis. Results: In all cases with expression of TAp63 and ΔNp63 variants, d4TAp63 and ΔNp73L variants were always expressed with their long variants simultaneously. In tumoral and marginal samples ΔNp73L mean expression level was significantly higher than the mean expression level of d4TAP63 (P = 0.009&P=0.008 respectively). ΔNp63 expression levels in tumoral and marginal samples were also higher than those of TAp63, which had a statistically significant difference in tumoral samples (P=0.03), but no significant difference was detected in marginal samples (P = 0.11). Conclusion: The results indicated dominant negative inhibitory activity of ΔNp63 and its potential role as an oncogene in breast cancer. ΔNp73L variant compared to d4TAp63, in tumoral and non tumoral breast tissues can act as dominant negative variant. Both variants (ΔNp73L and d4TAp63) with similar expression patterns to their respective longer variants (ΔNp63 and TAp63) and simultaneous expression with their variants are likely to be involved in strengthening the tumor suppressor activity in normal and tumoral breast cells. On the other hand, according to the expression patterns of ΔNp73L and d4TAp63 variants, they cannot be considered as molecular markers in the diagnosis of breast tumors. Keywords: Expression, TP63 gene, Breast cancer, Molecular markers, d4TAp63 variants, ΔNp73L variants. Received: Jun 8, 2013 Accepted: May 6, 2014
Keywords: Expression, TP63 gene, Breast cancer, Molecular markers, d4TAp63 variants, ΔNp73L variants.
Full-Text [PDF 359 kb]   (698 Downloads)    
Type of Study: Research | Subject: General
Received: 2014/11/10 | Accepted: 2014/11/10 | Published: 2014/11/10
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Volume 19, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2014) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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