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:: Volume 19, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2014) ::
SJKU 2014, 19(1): 78-87 Back to browse issues page
Generation of induced pluripotent stem cells from mesenchymal stem cells of human umbilical cord vein
Mehdad Abdi 1, Sona Zare 1, Fardin Fathi Dr * 2, Seyed Hadi Anjamrooz Dr3, Jafar Rezaei Dr3, Jalal Rostamzadeh Dr4
1- Kurdistan University of Medical Sciences
2- Professor Kurdistan University of Medical Sciences , farfath@gmail.com
3- Associate Professor Kurdistan University of Medical Sciences
4- Assistant Professor Kurdistan University of Medical Sciences
Abstract:   (6565 Views)
Background and Aim: The aim of this study was to generate induced pluripotent stem cells from human umbilical cord vein mesenchymal stem cells by use of plasmid vector. Material and Methods: In this experimental study using type IV collagenase enzyme, we extracted and cultured mesenchymal stem cells obtained from human umbilical cord vein wall.Usingelectroporation method, these cells were transfected with plasmid vector which carried selfrenewal transcription factors of OCT4 and SOX2.After 9 days we observed the induced pluripotent stem cells like colonies. The nature of thesecells were evaluated byimmunocytochemistry and alkalin phosphatase activity of embryonic stem cells. Results: Immunocytochemical analysis showed that these cells express the pluripotency markerssuch as OCT4, SSEA4, TRA1-60, TRA1-81, and also hadalkalin Phosphatase activity. Conclusion: This study revealed that transient expression of self renewal genes of OCT4 and SOX2 couldlead todevelopment of induced pluripotent stem cells like colonies from umbilical cord vein mesenchymal stem cells. Received: May 13, 2013 Accepted: Aug 25, 2013 Conflict of interest: None declared
Keywords: Embryonic stem cells, Umbilical cord vein mesenchymal stem cells, Reprogramming, Selfrenewal genes.
Full-Text [PDF 342 kb]   (1335 Downloads)    
Type of Study: Research | Subject: General
Received: 2014/03/16 | Accepted: 2014/03/16 | Published: 2014/03/16
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Volume 19, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2014) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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