[Home ] [Archive]   [ فارسی ]  
:: Volume 18, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2013) ::
SJKU 2013, 18(3): 29-38 Back to browse issues page
Identification of enterotoxigenic Escherichia coli strains in children under 2 years of age by real-time PCR in Shiraz
Mohammad Kargar Dr * 1, Pejman Abbasi 2, Abbas Doosti Dr3, Sadegh Ghorbani-Dalini 2
1- Associate Professor Islamic Azad University, Jahrom Branch , mkargar@jia.ac.ir
2- Islamic Azad University, Jahrom Branch
3- Assistant Professor Islamic Azad University, Shahrekord Branch
Abstract:   (6719 Views)
ABSTRACT Background and Aim: Enterotoxigenic strains of E. coli (ETEC) form the second largest group of diarrheagenic E. coli and are responsible for more than 25% of infantile diseases. The purpose of this study is to assess the prevalence of ETEC strains and their virulence genes in children less than 2 years of age in Shiraz. Material and Methods: This was a cross-sectional study. 285 stool samples were taken from children under 2 years of age with diarrhea in Shiraz. First, E. coli strains were isolated using standard biochemical tests. Then, prevalence of ETEC strains and presence of st and lt genes were evaluated using real-time PCR. Antibiotic susceptibility of the isolates was evaluated by using disc diffusion method. Results: 49 (17%) diarrheagenic E. coli strains were isolated, 7 cases (14/3%) belonged to ETEC strain. Among isolated ETEC strains, 4 (57.14%) had lt, 1 (14.29%) had stIa and 2 strains (28.57%) had both lt and stIa genes. All ETEC strains were susceptible to chloramphenicol, amikacin and nitrofurantoin, but showed resistance to penicillin and macrolides. Conclusion: The results showed high prevalence of ETEC strains in our study area. We recommend hospital-wide surveillance by using molecular techniques to estimate the prevalence of this pathotype in other regions of our country. Key words: Enterotoxigenic E. coli, Real-time PCR, Infants, Antibiotic resistance Received: Jun 17, 2012 Accepted: Apr 28, 2013 Conflict of interest: None declared
Keywords: Enterotoxigenic E. coli, Real-time PCR, Infants, Antibiotic resistance
Full-Text [PDF 293 kb]   (1342 Downloads)    
Type of Study: Research | Subject: General
Received: 2013/10/4
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print



Volume 18, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2013) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.08 seconds with 30 queries by YEKTAWEB 3731