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:: Volume 18, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2013) ::
SJKU 2013, 18(3): 13-19 Back to browse issues page
Effects of Nerium oleander leaf, Ricinus communis oil, Capsicum spp. seeds, and almond compound on cutaneous leishmaniasis caused by Leishmania species under laboratory condition and its effect on cutaneous lesion progression in mice
Mohammad Yakhchali Dr 1, Mehdi Ranjbariki-Jandabeh 2
1- Associate Professor Faculty of Veterinary Medicine, Urmia University , m.yakhchali@urmia.ac.ir
2- Private sector, Isfahan
Abstract:   (11241 Views)
ABSTRACT Background and Aim: Protozoan parasite of genus Leishmania is the causative agent of leishmaniasis in humans, carnivorous animals and rodents. In Iran, Leishmania species are causes of cutaneous leishmaniasis which had been reported frequently from Isfahan. The aim of the present study was to evaluate the effects of a compound of Nerium oleander leaf, Ricinus communis oil, Capsicum spp. seeds, and almond on cutaneous leishmaniasis caused by Leishmania species under laboratory conditions and its effect on the progression of cutaneous lesion in mice. Material and Methods: In this repeated experimental study, we obtained smears from the skin lesions of 10 patients with cutaneous leishmaniasis randomly. 3 cases had positive smears (30%) which were cultured in NNN media with 10% FBS to obtain promastigotes of leishmania. The effects of boiled and sieved herbal compound (Nerium oleander leaf, Ricinus communis oil, Capsicum spp. seeds, and almond) on the promastigote propagation in NNN media with 10% FBS (5×104/μl) were investigated in triplicate and compared with control group. In the 4th week, 0.2 ml of enriched promastigote of Leishmania in RPMI was inoculated in the base of the tails of the mice in the test group (n=6) and control group (n=2). The effect of herbal compound on the progression of the lesion was investigated for 7 weeks. We used t-test for statistical analysis. Results: The number of Leishmania promastigotes in the group treated with herbal compound was significantly less than that in the control group. Also the herbal compound had significant preventive effect on the progression of the cutaneous lesion in the test group as compared to the control group. Conclusion: The results of this study showed that the herbal compound had preventive effect on the parasite propagation and progression of cutaneous lesion. We recommend furthur studies on other pharmacological aspects of this herbal compound. Keywords: Nerium oleander, Leishmania spp., Promastigote, Iran. Received: Oct 30, 2012 Accepted: Jun 9, 2013 Conflict of interest: None declared
Keywords: Nerium oleander, Leishmania spp., Promastigote, Iran
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Type of Study: Research | Subject: General
Received: 2013/10/4
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Volume 18, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2013) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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