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:: Volume 18, Issue 2 (Scientific Journal of Kurdistan Universiy of Medical Sciences 2013) ::
SJKU 2013, 18(2): 114-120 Back to browse issues page
Frequency of sialic acid binding adhesin gene in Helicobacter pylori isolated from patient with gastroduodenal diseases
Zahra Pakbaz 1, Mohammad Hasan Shirazi Dr 2, Mohammad Reza Pourman Dr3, Reza Ranjbar Dr4, Mostafa Hoseini Dr5, Ziba Vaise Malekshahi , Sara Haji Khani 1
1- Tehran University of Medical Sciences
2- Associate Professor Tehran University of Medical Sciences , mhshirazi@tums.ac.ir
3- Associate Professor Tehran University of Medical Sciences
4- Associate Professor Baqiyatallah University of Medical Sciences
5- Professor Tehran University of Medical Sciences
Abstract:  
ABSTRACT Background and Aim: Sialic acid binding adhesin gene is one of the most important factors contributing to adhesin of Helicobacter pylori (H. pylori) to epithelial cell layer of stomach. The prevalence rates of sialic acid binding adhesin gene vary in different geographic areas. The aim of this study was to determine the frequency of sialic acid binding adhesin coding gene in the patients with different gastroduodenal diseases. Materials and Methods: This is a cross-sectional study. One hundred twenty patients with GI symptoms were enrolled in this study. Two gastric biopsy specimens were taken from each of the patients for rapid urease test (RUT) and DNA extraction. Presence of H. pylori was investigated by RUT and urease A gene (ureA) PCR. sialic acid binding adhesin gene was detected by using gene specific primers. Results: Among 120 samples, presence of H. pylori was confirmed in 82 cases, of which 64 strains (78%) were positive for sialic acid binding adhesin gene. The frequency of this gene was 84.6%, 86.7%, 77.8% and 72.2% for gartric cancer, gastric ulcer, duodenal ulcer and gastritis (%66.7) respectively. Conclusion: The frequency of sialic acid binding adhesin gene in different samples was almost the same. Discrepancies in the frequency of this gene in different studies may be related to geographical diversity or use of different primers for detection of this gene. Conflict of Interest: Nil Received: Apr 6, 2012 Accepted: Sep 27, 2012
Keywords: Helicobacter pylori, Sialic acid binding adhesin, Gastrointestinal disease.
Full-Text [PDF 609 kb]      
Type of Study: Research | Subject: General
Received: 2013/06/18
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Volume 18, Issue 2 (Scientific Journal of Kurdistan Universiy of Medical Sciences 2013) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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