[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Journal Citation Index

 

Citation Indices from GS

AllSince 2019
Citations89785395
h-index3725
i10-index235137

 

..
Central Library of Kurdistan University of Medical Sciences
AWT IMAGE
..
Vice-Chancellery for Research and Technology
AWT IMAGE
..
SCImago Journal & Country Rank
:: Volume 18, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2013) ::
SJKU 2013, 18(1): 47-58 Back to browse issues page
Study of the photocatalytic effects of nitrogen-doped titanium dioxide nanoparticles on growth inhibition and apoptosis induction in K562 cell line
Seyed Mohammad Amin Moosavi 1, Alireza Khataee2 , Soroosh Moasses Ghafari2
1- The University of Tabriz , moosav_m@tabrizu.ac.ir
2- The University of Tabriz
Abstract:   (12202 Views)
Background and Aim: Titanium dioxide (TiO2) nanoparticles have shown anti-tumor activity in several cancer cell lines. TiO2 is an effective photocatalyst that needs ultraviolet (UV) light for activation. Many efforts are in progress to improve the TiO2 photocatalytic effects by carrying out superficial alterations in its electronic structure. For this purpose, nitrogen (N) element seems to be a proper dopant to improve photocatalytic activity of this nanoparticle. In the present study, the growth inhibitory and apoptotic effects of N-doped TiO2 nanoparticles on human K562 cells, as an experimental model of chronic myeloid leukemia (CML), were investigated and compared with those of undoped TiO2 in both light and dark conditions. Material and Methods: Nanocrystalline powders of N-doped TiO2 (80 nm) were synthesized by mechanical mixing of urea with TiO2 powders under special experimental conditions. The structure and properties of N-doped TiO2 nanoparticles were confirmed by transmission electron microscopy (TEM), X-ray diffraction (XRD) and Brunauer–Emmett–Teller (BET) analysis. Then we investigated the growth inhibitory and apoptotic effects of these nanoparticles on leukemia K562 cells. Results: The results showed that visible light-irradiated N-doped TiO2 induced profound growth inhibition and apoptosis in K562 cells in a time- and dose- dependent manner. For example, N-doped TiO2 at concentrations of 0.01µg/ml, 0.1µg/ml, 1 µg/ml, 5 µg/ml and 10µg/ml inhibited growth of K562 cells by 25%, 34%, 39%, 44% and 47% and viability by 11%, 22%, 30%, 35% and 38%, respectively after 48h. Furthermore, the results of DNA fragmentation assay confirmed apoptosis in treated-K562 cells after 48h. While un-doped TiO2 did not show any inhibitory effects on the growth and viability of K562 cells. Conclusion: Considering the growth inhibitory and apoptotic effects of N-doped TiO2 in human K562 cells, this nanoparticle can be a potential candidate for photocatalytic therapy in CML in the future studies.
Keywords: Apoptosis, K562, Nitrogen- doped titanium dioxid, Photocatalysis
Full-Text [PDF 1044 kb]   (6569 Downloads)    
Type of Study: Original Research | Subject: General
Received: 2013/04/27 | Published: 2013/05/15
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Moosavi S M A, Khataee A, Moasses Ghafari S. Study of the photocatalytic effects of nitrogen-doped titanium dioxide nanoparticles on growth inhibition and apoptosis induction in K562 cell line. SJKU 2013; 18 (1) :47-58
URL: http://sjku.muk.ac.ir/article-1-1007-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2013) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 45 queries by YEKTAWEB 4645