|
Evaluation of the Effect of Farnesol and/or Chitosan as a Final Irrigation on Enterococcus faecalis Biofilm; An In-vitro Study
|
Ardavan Moinafshar1    , Hanieh Paik2 , Rashid Ramazanzadeh3 , Amjad Ahmadi4 , Mohammad Rastegar Khosravi 5 |
1- Kurdistan University of Medical Sciences, Sanandaj, Iran.
2- Kurdistan University of Medical Sciences, Sanandaj, Iran
3- Department of Microbiology, School of Medicine and Researcher in Cellular & Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
4- Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
5- Department of Endodontics, Faculty of Dentistry, Kurdistan University of Medical Sciences, Sanandaj, Iran , mr.khosravi@muk.ac.ir |
|
|
Abstract: (953 Views) |
Background and Aim: Complete cleaning of the tooth root canal plays an important role in the success of endodontic treatments, which can be done with the help of mechanical and chemical methods. Two antibacterial detergents, farnesol and chitosan, have been proposed for removal and reduction of microbial species. The aim of this study was to investigate the antibacterial effect of farnesol and/or chitosan on Enterococcus faecalis biofilm.
Materials and Methods: In this experimental-laboratory study, the standard strain of Enterococcus faecalis was cultured in BHI broth medium for 7 and 21 days. Then, the biofilms were exposed to farnesol and chitosan as the antimicrobial substances. The antimicrobial effects of these substances on the growth of biofilm were investigated by analyzing the color spectrum of Confocal Laser Scanning Microscopy images.
Results: In comparison to the positive control group, farnesol reduced the number of live bacteria by 54.67% and 36.54% in the biofilms formed after 7 and 21 days, respectively. These figures were 64.08% and 39.05%, for chitosan respectively. The combination of farnesol and chitosan was able to reduced live bacteria by 82.77% and 60.91% in 7 and 21 day biofilm, respectively.
Conclusion: The results of this study showed that use of farnesol and chitosan alone or in combination could destroy the living cells of Enterococcus faecalis biofilm. Combination of farnesol and chitosan had the greatest effect on destroying biofilm bacteria in both groups. Therefore, we recommend use of the combination of farnesol and chitosan for irrigation of the root canal, especially in the re-treatment of the root and teeth with periapical lesions.
|
|
| Keywords: Enterococus faecalis, Biofilm, Farnesol, Chitosan |
|
|
Full-Text [PDF 925 kb]
(138 Downloads)
|
Type of Study: Applicable |
Subject:
Dentistry
Received: 2022/11/28 | Accepted: 2023/09/5 | Published: 2024/04/16
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
