[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Journal Citation Index

 

Citation Indices from GS

AllSince 2019
Citations93245682
h-index3826
i10-index243146

 

..
Central Library of Kurdistan University of Medical Sciences
AWT IMAGE
..
Vice-Chancellery for Research and Technology
AWT IMAGE
..
SCImago Journal & Country Rank
:: Volume 28, Issue 6 (Scientific Journal of Kurdistan University of Medical Sciences 2024) ::
SJKU 2024, 28(6): 1-11 Back to browse issues page
Effects of Dopamine and L-dopa on Ghrelin Gene Expresion in the Hypothalamus and Ovary in a Polycystic Ovarian Syndrome Rat Model
Leila Neghaddadgar1 , Fariba Mahmoudi 2, Homayoun Khazali3
1- Department of Biology / Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran
2- Department of Biology / Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran , f.mahmoudi@uma.ac.ir
3- Department of Animal Sciences and Marine Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
Abstract:   (875 Views)
Background and Aim: In healthy people, dopamine stimulates ghrelin secretion. The level of dopamine release  and ghrelin is lower in the patients with polycystic ovary syndrome (PCOS). In the present study,we investigated the effects of doplamin and L-dopa on relative gene expression of ghrelin in PCOS model rats.
Materials and Methods: In the first step of the study, PCOS was induced in 25 female Wistar rat (Rattus norvegicus) by injection of estradiol. Then, the rats received saline, dopamine (5µg), L-dopa (5µg) or simultaneous injections of sulpride (10µg/kg), SCH23390 hydrochloride (10µg/kg) and dopamine or L-dopa respectively via third cerebral ventricular. In the second part of the study, 15 PCOS rats received saline L-dopa (100mg/kg) or dopamine (50mg/kg) intraperitoneally. Hypothalamic and ovarian samples were dissected. Mean relative ghrelin gene expression was determined by real- time-PCR method. 
Results: Mean relative gene expression of ghrelin significantly decreased in the hypothalamus and ovary of the rats with PCOS compared to those in the intact rats. Intraventricular injection of dopamine or L-dopa significantly increased the hypothalamic ghrelin gene expression in comparison to that in the rats in the PCOS group. Dopamine or L-dopa did not significantly increase the gene expression of ovarian ghrelin in comparison to that in PCOS group. Injections of sulpride and SCH23390 significantly blocked the stimulatory effects of dopamine or L-dopa on the ghrelin gene expression in hypothalamus compared to those in the dopamine or L-dopa group.
Conclusion: The dopaminergic pathway may be involved in increasing ghrelin gene expresion extremely via hypothamic level in PCOS condition.
 
Keywords: Dopamine, L-dopa, Ghrelin, Polycystic ovary syndrome.
Full-Text [PDF 659 kb]   (247 Downloads)    
Type of Study: Original Research | Subject: Physiology and neurosciences
Received: 2022/05/21 | Accepted: 2022/11/28 | Published: 2024/02/6
References
1. Sadeghi HM, Adeli I, Calina D, Docea AO, Mousavi T, Daniali M, et al.. Polycystic ovary syndrome: A Comprehensive review of pathogenesis, management, and drug repurposing. Int J Mol Sci . 2022; 23(2): 583. [DOI:10.3390/ijms23020583] [PMID] []
2. Liu x, Herbison A. Dopamine regulation of gonadotropin-releasing hormone neuron excitability in male and female mice. Endocrinology 2013; 154(1): 340-50. [DOI:10.1210/en.2012-1602] [PMID]
3. Björklund A, Dunnett SB. Dopamine neuron systems in the brain: an update. Trends Neurosci. 2007; 30(5):194-202. [DOI:10.1016/j.tins.2007.03.006] [PMID]
4. Jaber M, Robinson SW, Missale C, Caron MG. Dopamine receptors and brain function. Neuropharmacology. 1996; 35(11):1503-19. [DOI:10.1016/S0028-3908(96)00100-1] [PMID]
5. Romero-Picó A, Novelle MG, Folgueira C, López M, Nogueiras R, Diéguez C. Central manipulation of dopamine receptors attenuates the orexigenic action of ghrelin. Psychopharmacology. 2013; 229(2): 275-83 [DOI:10.1007/s00213-013-3096-7] [PMID]
6. Goshima Y, Masukawa D, Kasahara Y, Hashimoto T. L-DOPA and its receptor GPR143: implications for pathogenesis and therapy in parkinson's disease. Front Pharmacol. 2019; 10:1119. [DOI:10.3389/fphar.2019.01119] [PMID] []
7. Schalla MA, Stengel A. The role of the gastric hormones ghrelin and nesfatin-1 in reproduction. Int J Mol Sci. 2021; 22(20): 11059. [DOI:10.3390/ijms222011059] [PMID] []
8. Caminos JE, Tena-Sempere M, Gaytán F, Sanchez-Criado JE., Barreiro ML, Nogueiras R, et al. Expression of ghrelin in the cyclic and pregnant rat ovary. Endocrinology. 2003; 144: 1594-1602. [DOI:10.1210/en.2002-221058] [PMID]
9. Pagotto U, Gambineri A, Vicennati V, Heiman ML, Tschöp M, Pasquali R. Plasma ghrelin, obesity, and the polycystic ovary syndrome: Correlation with insulin resistance and androgen levels. J Clin Endocrinol Metab. 2002; 87:5625-29. [DOI:10.1210/jc.2002-020776] [PMID]
10. Sadeghzadeh A, Bayrami A, Mahmoudi F, Khazali H, Asadi A. The effects of interaction of dopaminergic and kisspeptin neural pathways on ghrelin secretion in rats. J Paramed Sci. 2018; 9(1):29-35.
11. Gómez R, Ferrero H, Delgado-Rosas F, Gaytan M, Morales C, Zimmermann RC, Simón C, et al. Evidences for the existence of a low dopaminergic tone in polycystic ovarian syndrome: implications for OHSS development and treatment. J Clin Endocrinol Metab. 2011; 96(8): 2484-92. [DOI:10.1210/jc.2011-0075] [PMID]
12. Haghighat Gollo K, Mahmoudi F, Bayrami A, Zahri S. Influences of L-DOPA and blocking dopamine receptors on aromatase gene expression and serum concentration of LH in polycystic ovary syndrome model rats. JABS. 2020; 10(3):2448-55.
13. Lhosrobakhsh F, Moloudi MR, Shoja K, Mohammadi S. Effect of alpha- lipoic acid on pancreatic optic atrophy 1(OPA1) gene expression in male rat model of obstructive cholestasis and cirrhosis. SJKU. 2019; 24(5):120-34. [DOI:10.29252/sjku.24.5.120]
14. Khazali H, Mahmoudi F, Janahmadi M. Hypothalamic KiSS1/GPR54 gene expressions and luteinizing hormone plasma secretion in morphine treated male rats. Int J Fertil Steril. 2018; 12(3):223-8.
15. Gambineri A, Pagotto U, Tschöp M, Vicennati V, Manicardi E, Carcello A, et al. Anti-androgen treatment increases circulating ghrelin levels in obese women with polycystic ovary syndrome. J Endocrinol Invest. 2003; 26(7): 629-34. [DOI:10.1007/BF03347020] [PMID]
16. Akalu Y, Derbew Molla M, Dessie G, Ayelign B. Effect of ghrelin on body systems. Int J Endocrinol. 2020; 2020: 1385138. [DOI:10.1155/2020/1385138] [PMID] []
17. Tang R, Ding X, Zhu J. Kisspeptin and polycystic ovary syndrome. Front Endocrinol (Lausanne). 2019; 10: 298. [DOI:10.3389/fendo.2019.00298] [PMID] []
18. Iwakura H, Ariyasu H, Hosoda H, Yamada G, Hosoda K, Nakao K, et al. Oxytocin and dopamine stimulate ghrelin secretion by the ghrelin-producing cell line, MGN3-1 in vitro. Endocrinology. 2011; 152(7): 2619-25. [DOI:10.1210/en.2010-1455] [PMID]
19. Weinberg ZY, Nicholson ML, Currie. PJ. 6-Hydroxydopamine lesions of the ventral tegmental area suppress ghrelin's ability to elicit food-reinforced behavior. Neurosci Lett. 2011; 499(2): 70-3. [DOI:10.1016/j.neulet.2011.05.034] [PMID]
20. Jiang H, Betancourt L, Smith RG. Ghrelin amplifies dopamine signaling by cross talk involving formation of growth hormone secretagogue receptor/dopamine receptor subtype 1 heterodimers. Mol Endocrinol. 2006; 20(8):1772-85. [DOI:10.1210/me.2005-0084] [PMID]
21. Romero-Picó A, Novelle MG, Folgueira C, López M, Nogueiras R, Diéguez C. Central manipulation of dopamine receptors attenuates the orexigenic action of ghrelin. Psychopharmacology (Berl). 2013; 229(2): 275-83. [DOI:10.1007/s00213-013-3096-7] [PMID]
22. Jerlhag E, Egecioglu E, Dickson SL, Douhan A, Svensson L, Engel JA. Ghrelin administration into tegmental areas stimulates locomotor activity and increases extracellular concentration of dopamine in the nucleus accumbens. Addict Biol. 2007; 12(1): 6-16. [DOI:10.1111/j.1369-1600.2006.00041.x] [PMID]
23. Blázquez E, Velázquez E, Hurtado-Carneiro V, Miguel Ruiz-Albusac J. Insulin in the brain: its pathophysiological implications for states related with central insulin resistance, type 2 diabetes and Alzheimer's disease. Front Endocrinol (Lausanne). 2014; 5: 161. [DOI:10.3389/fendo.2014.00161] [PMID] []
24. Ustione A, Piston DW, Harris PE. Minireview: Dopaminergic regulation of insulin secretion from the pancreatic islet. Mol Endocrinol. 2013; 27(8): 1198-207. [DOI:10.1210/me.2013-1083] [PMID] []
25. Pöykkö SM, Kellokoski E, Hörkkö S, Kauma H, Kesäniemi YA, Ukkola O. Low plasma ghrelin is associated with insulin resistance, hypertension, and the prevalence of type 2 diabetes. Diabetes. 2003; 52(10): 2546-53. [DOI:10.2337/diabetes.52.10.2546] [PMID]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

Ethics code: IR.ARUMS.REC.1400.085



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Neghaddadgar L, Mahmoudi F, Khazali H. Effects of Dopamine and L-dopa on Ghrelin Gene Expresion in the Hypothalamus and Ovary in a Polycystic Ovarian Syndrome Rat Model. SJKU 2024; 28 (6) :1-11
URL: http://sjku.muk.ac.ir/article-1-7361-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 28, Issue 6 (Scientific Journal of Kurdistan University of Medical Sciences 2024) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 44 queries by YEKTAWEB 4652