fa تأثیر فعالیت بدنی بر بیان ژن ایندول‌آمین 3،2-دی‌اکسیژناز1 و آریل‌آلکیل‌آمین N-استیل ترانسفراز در مخچه موش‌های ماده مبتلا به آنسفالومیلیت خود ایمن تجربی تربیت بدنی Physical Education پژوهشي اصیل Original Research <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="FA" style="font-family:" zar="">ز</span></b></span></span></span></span></span></span></span><span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">زمینه و هدف:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> القاء آنسفالومیلیت خود‌ایمن تجربی (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">EAE</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">) با افزایش بیش از حد بیان ایندول‌آمین 3،2-دی اکسیژناز 1 (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IDO1</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">) و تشدید علائم بالینی همراه است. این مطالعه با هدف بررسی تأثیر فعالیت بدنی بر بیان ژن </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IDO1</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> و آریل آلکیلآمین </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">N</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">_استیل ترانسفراز (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">AANAT</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">) در مخچه موش‌های مبتلا به </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">EAE</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> انجام شد.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">مواد و روش&shy;ها:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> 30 موش </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">C57BL/6</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> به طور تصادفی به سه گروه 10 تایی کنترل سالم، کنترل </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">EAE</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> و فعالیت در محیط غنی‌شده تقسیم شدند. پس از القاء </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">EAE</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> با </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">MOG_35-55</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">به مدت 4 هفته در محیط غنی شده قرارگرفتند و سپس بافت مخچه تشریح شد و بیان ژن‌های </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IDO1</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">AANAT</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> به روش&nbsp; </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">&nbsp;Real</span> <span dir="LTR" new="" roman="" style="font-family:" times="">Time</span><span dir="LTR" style="font-size:12.0pt"><span arial="" style="font-family:">-</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">PCR</span><span b="" style="font-family:" zar="">در</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> هر سه گروه اندازه‌گیری شد. از آزمون تجزیه‌وتحلیل واریانس در سطح معنی‌داری کمتر از 0/05 برای آنالیز آماری داده‌ها استفاده شد.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">&nbsp;یافته‌ها:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> فعالیت در محیط غنی شده در مقایسه با گروه کنترل </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">EAE</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> بیان ژن </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IDO1</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> را به طور معنی‌داری کاهش داد (0/02 = </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">)، بیان ژن </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">AANAT</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> را نیز به طور معنی‌داری کاهش داد (0/04 = </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">) و همچنین نمره بالینی را به طور معنی‌داری کاهش داد (0/001 = </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">).</span></span></span></span></span></span></span></span></span><br> <b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" style="font-family:" zar="">نتیجه‌گیری:</span></span></span></b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" style="font-family:" zar=""> تغییر محیط و سبک زندگی فعال در محیطی غنی‌شده می‌تواند از طریق تنظیم کاهشی بیان ژن‌</span></span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:107%"><span b="" style="font-family:" zar="">های</span></span></span><span style="font-size:11.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">IDO1</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" style="font-family:" zar=""> و </span></span></span><span style="font-size:11.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">AANAT</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" style="font-family:" zar=""> از پیشرفت بیماری اتو ایمن </span></span></span><span style="font-size:11.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">MS</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" style="font-family:" zar=""> پیشگیری کند.</span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:right 99.25pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background and Aim:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Induction of experimental autoimmune encephalomyelitis (EAE) is associated with an overexpression of indoleamine 2,3-dioxygenase 1 (IDO1) and exacerbation of clinical symptoms. This study aimed to investigate the effect of physical activity on the gene expression of IDO1 and arylalkylamine N-acetyltransferase (AANAT) in the cerebellum of the mice with EAE.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods:<i> </i></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;30 C57BL/6 mice were randomly divided into three groups of 10: healthy control, EAE control, and physical activity in the enriched environment. Mice were placed in an enriched environment after induction of EAE with MOG_35-55 for 4 weeks and then the cerebellum tissue was dissected and the expression of IDO1 and AANAT genes were measured by real time-PCR method in all three groups. Analysis of variance test was used for data analysis at a significance level of less than 0.05.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:<i> </i></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The group</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> with physical activity in an enriched environment showed significant reduction in the gene expression of IDO1 (P = 0.02) and AANAT (P = 0.04) genes, and also significantly reduced the clinical score (P = 0.001) compared to the EAE group.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;Changing the environment and lifestyle in the enriched environment can prevent the progression of the autoimmune diseases such as MS, through downregulation of the expression of the IDO1 and AANAT genes.</span></span></span></span></span></span><br> &nbsp; ایندول آمین 3,2-دی اکسیژناز 1, آریل آلکیلآمین N_استیل ترانسفراز, مالتیپل اسکلروزیس, فعالیت ورزشی Indoleamine 2,3-dioxygenase 1, Arylalkylamine N-acetyltransferase, Multiple sclerosis, Physical activity 12 23 http://sjku.muk.ac.ir/browse.php?a_code=A-10-7341-1&slc_lang=fa&sid=1 samira emadi سمیرا عمادی emadisamira@yahoo.com 5400319475328460063262 5400319475328460063262 No Ph.D Student of Exercise Physiology, Department of Sport Science, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran دانشجو دکتری فیزیولوژی ورزشی، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران Shahnaz Shahrbanian شهناز شهربانیان sh.shahrbanian@modares.ac.ir 5400319475328460063263 5400319475328460063263 Yes Associate Professor, Department of Sport Science, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran دانشیار، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران Mohammadreza Kordi محمدرضا کردی m.kordi@iut.ac.ir 5400319475328460063264 5400319475328460063264 No Professor, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran. استاد، گروه فیزیولوژی ورزشی، دانشکده تربیت‌بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران Mahdieh Molanouri Shamsi مهدیه ملانوری شمسی molanouri@modares.ac.ir 5400319475328460063265 5400319475328460063265 No Associate Professor, Department of Sport Science, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran. دانشیار، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران