fa اثر تحریک گیرنده 5-HT2A بر علائم ترک اعتیاد به مرفین در موش سوری فیزیولوژی و علوم اعصاب Physiology and neurosciences پژوهشي اصیل Original Research <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" style="font-family:" zar="">زمینه و هدف:</span></span></b> <span b="" lang="FA" style="font-family:" zar="">سیستم سروتونرژیک از طریق گیرنده</span><span dir="LTR" style="font-family:BLotusBold">&shy;</span><span b="" lang="FA" style="font-family:" zar="">های خود</span> <span b="" lang="FA" style="font-family:" zar="">در روند وابستگی و بروز علائم سندرم محرومیت مرفین نقش دارد. از سوی</span> <span b="" lang="FA" style="font-family:" zar="">دیگر اثر</span> <span b="" lang="FA" style="font-family:" zar="">گیرنده</span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:BLotusBold">5-HT2A</span></span><span b="" lang="FA" style="font-family:" zar=""> بر علائم</span> <span b="" lang="FA" style="font-family:" zar="">ترک</span> <span b="" lang="FA" style="font-family:" zar="">اعتیاد</span> <span b="" lang="FA" style="font-family:" zar="">به‌طور کامل شناخته نشده است</span><span dir="LTR" style="font-family:BLotusBold">.</span><span b="" style="font-family:" zar=""> هدف از این مطالعه بررسی اثر تحریک گیرنده </span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:BLotusBold">5-HT2A</span></span><span b="" lang="FA" style="font-family:" zar=""> بر علائم ترک مورفین در موش های سوری نر است.</span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" style="font-family:" zar="">مواد و روش&shy; ها:</span></span></b> <span b="" lang="FA" style="font-family:" zar="">در</span> <span b="" lang="FA" style="font-family:" zar="">این</span> <span b="" lang="FA" style="font-family:" zar="">مطالعه</span> <span b="" lang="FA" style="font-family:" zar="">تجربی</span> <span b="" lang="FA" style="font-family:" zar="">از</span> <span b="" lang="FA" style="font-family:" zar="">28 سر</span> <span b="" lang="FA" style="font-family:" zar="">موش</span> <span b="" lang="FA" style="font-family:" zar="">سوری</span> <span b="" lang="FA" style="font-family:" zar="">نر</span> <span b="" lang="FA" style="font-family:" zar="">نژاد</span> <span dir="LTR" style="font-size:10.0pt">NMRI</span><span b="" lang="FA" style="font-family:" zar=""> (20 الی 25 گرم) استفاده</span> <span b="" lang="FA" style="font-family:" zar="">شد. وابستگی</span> <span b="" lang="FA" style="font-family:" zar="">به</span> <span b="" lang="FA" style="font-family:" zar="">مرفین با استفاده از برنامه</span> <span b="" lang="FA" style="font-family:" zar="">5</span> <span b="" lang="FA" style="font-family:" zar="">روزه</span> <span b="" lang="FA" style="font-family:" zar="">و</span> <span b="" lang="FA" style="font-family:" zar="">به</span> <span b="" lang="FA" style="font-family:" zar="">ترتیب</span> <span b="" lang="FA" style="font-family:" zar="">با دوزهای 6، 16، 26، 36، 46، 56 و 66 میلی‌گرم</span> <span b="" lang="FA" style="font-family:" zar="">بر</span> <span b="" lang="FA" style="font-family:" zar="">کیلوگرم</span> <span b="" lang="FA" style="font-family:" zar="">القاء</span> <span b="" lang="FA" style="font-family:" zar="">شد. در</span> <span b="" lang="FA" style="font-family:" zar="">روز</span> <span b="" lang="FA" style="font-family:" zar="">پنجم</span> <span b="" lang="FA" style="font-family:" zar="">دو ساعت پس از</span> <span b="" lang="FA" style="font-family:" zar="">تک</span> <span b="" lang="FA" style="font-family:" zar="">دوز</span> <span b="" lang="FA" style="font-family:" zar="">مرفین؛</span> <span b="" lang="FA" style="font-family:" zar="">نالوکسان (3 میلی گرم بر کیلوگرم) تزریق</span> <span b="" lang="FA" style="font-family:" zar="">شده</span> <span b="" lang="FA" style="font-family:" zar="">و</span> <span b="" lang="FA" style="font-family:" zar="">علائم</span> <span b="" lang="FA" style="font-family:" zar="">ترک</span> <span b="" lang="FA" style="font-family:" zar="">با تعداد پرش، دندان قروچه، ریتینگ، لرزش سر، لرزش اندام و درصدکاهش وزن در</span> <span b="" lang="FA" style="font-family:" zar="">طی 30 دقیقه ثبت شد. گروه</span> <span b="" lang="FA" style="font-family:" zar="">درمان،</span> <span b="" lang="FA" style="font-family:" zar="">دوزهای</span> <span b="" lang="FA" style="font-family:" zar="">مختلف </span><span dir="LTR" style="font-size:10.0pt">TCB-2</span> <span b="" lang="FA" style="font-family:" zar="">(0/3 و 2/5 میلی‌گرم</span> <span b="" lang="FA" style="font-family:" zar="">بر</span> <span b="" lang="FA" style="font-family:" zar="">کیلوگرم؛</span> <span b="" lang="FA" style="font-family:" zar="">به</span> <span b="" lang="FA" style="font-family:" zar="">شکل داخل</span> <span b="" lang="FA" style="font-family:" zar="">صفاقی) را</span> <span b="" lang="FA" style="font-family:" zar="">دریافت کردند</span><span lang="FA" style="font-size:11.0pt"><span b="" style="font-family:" zar="">.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span b="" style="font-family:" zar="">یافته‌ها:</span></span></span></b> <span lang="FA" style="line-height:115%"><span b="" style="font-family:" zar="">موش‌های وابسته به مورفین به طور قابل توجهی علائم ترک بیشتری نسبت به موش‌های کنترل داشتند. دوزهای مختلف </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:BLotusBold">TCB-2</span></span></span><span lang="FA" style="line-height:115%"><span b="" style="font-family:" zar=""> به طور معنی‌داری برخی از علائم ترک مورفین را در گروه</span></span><span dir="LTR" style="line-height:115%"><span style="font-family:BLotusBold">&shy;</span></span><span lang="FA" style="line-height:115%"><span b="" style="font-family:" zar="">های آزمایشی افزایش داد.</span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" style="font-family:" zar="">نتیجه‌گیری:</span></span></b> <span b="" lang="FA" style="font-family:" zar="">مطالعه حاضر نشان داد که تحریک گیرنده </span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:BLotusBold">5-HT2A</span></span> <span b="" lang="FA" style="font-family:" zar="">از طریق آگونیست آن علائم ترک مورفین را تشدید می‌کند.</span> <span b="" lang="FA" style="font-family:" zar="">مطالعات بیشتر برای درک بهتر نقش گیرنده </span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:BLotusBold">5-HT2A</span></span> <span b="" lang="FA" style="font-family:" zar="">در وابستگی و ترک مرفین توصیه می</span><span dir="LTR" style="font-size:13.0pt"><span style="font-family:BLotusBold">&shy;</span></span><span b="" lang="FA" style="font-family:" zar="">شود</span><span dir="LTR" style="font-size:13.0pt"><span style="font-family:BLotusBold">.</span></span><span lang="FA" style="font-family:"B Zar""></span></span></span></span></span></span><br> &nbsp; <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Background and Aim:</b> Serotonergic system has a role in morphine dependence and withdrawal <span style="font-family:BZarBold">symptoms<b> </b></span>via its receptors. On the other hand, the effect of 5-HT2A receptor on withdrawal <span style="font-family:BZarBold">symptoms<b> </b></span>has not been evaluated completely. The aim of this study was to investigate the effect of 5-HT2A receptor stimulation on morphine withdrawal <span style="font-family:BZarBold">symptoms<b> </b></span>in male mice.</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Materials and Methods:<i> </i></b>This experimental study included twenty eight male NMRI mice (20-25g). Morphine dependency was induced in a five- day schedule by 6, 16, 26, 36, 46, 56 and 66 mg/kg doses respectively. On the fifth day, 2 hours after a single dose of morphine, naloxone was injected (3 mg/kg) and the scores (from 0 to 3) of withdrawal <span style="font-family:BZarBold">symptoms<b> </b></span>including number of jumps, teeth chattering, writing, head shakes, limbs shakes, and percentage of body weight loss were recorded for 30min.The treatment group received different doses of TCB-2 (03, and 2.5mg/kg; Intraperitoneally).</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Results:</b> Withdrawal symptoms in the morphine-dependent mice were significantly more frequent than those in the control mice. Different doses of TCB-2 significantly increased some of the morphine withdrawal <span style="font-family:BZarBold">symptoms<b> </b></span>in the experimental groups.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Conclusion: </b>The present study indicated that stimulation of 5-HT2A receptor via its agonist exacerbated the morphine withdrawal <span style="font-family:BZarBold">symptoms</span>. Further studies are recommended to better understand the role of the 5-HT2A receptor in morphine dependence and withdrawal.</span></span></span></span><br> <br> <span style="font-size:12pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">&nbsp;</span></span></span></span></span><br> <br> <br> &nbsp; گیرنده 5-HT2A, مرفین, سندرم محرومیت, موش سوری 5-HT2A receptor, Morphine, Withdrawal syndrome, Mice 40 50 http://sjku.muk.ac.ir/browse.php?a_code=A-10-6757-1&slc_lang=fa&sid=1 Nasrin Hashemi-Firouzi نسرین هاشمی فیروزی nhashemi@umsha.ac.ir 5400319475328460055264 5400319475328460055264 No Associate Professor, Neurophysiology Research Center, Hamadan University of medical Science, Hamadan, Iran استادیار مرکز تحقیقات فیزیولوژی اعصاب، دانشگاه علوم پزشکی همدان، همدان، ایران. Siamak Shahidi سیامک شهیدی siamakshahidi@gmail.com 5400319475328460055265 5400319475328460055265 No Professor, Department of Physiology and Neurophysiology Research Center, Hamadan University of medical Science, Hamadan, Iran. استاد، گروه فیزیولوژی و مرکز تحقیقات فیزیولوژی اعصاب، دانشگاه علوم پزشکی همدان، همدان، ایران. Simin Afshar سیمین افشار afshar_biology@yahoo.com 5400319475328460055266 5400319475328460055266 No Assistant Professor, Neurophysiology Research Center, Hamadan University of medical Science, Hamadan, Iran استادیار مرکز تحقیقات فیزیولوژی اعصاب، دانشگاه علوم پزشکی همدان، همدان، ایران Parisa habibi پریسا حبیبی dr.habibi2007@yahoo.com 5400319475328460055267 5400319475328460055267 No Assistant Professor, Department of Physiology, Hamadan University of medical Science, Iran استادیار؛ گروه فیزیولوژی، دانشگاه علوم پزشکی همدان، همدان، ایران. Mahdi Ramezani مهدی رمضانی m.ramezani@umsha.ac.ir 5400319475328460055268 5400319475328460055268 Yes Assistant Professor, Anatomical Sciences, Hamadan University of medical Science, Hamadan, Iran استادیار، گروه آناتومی، دانشگاه علوم پزشکی همدان، همدان، ایران.