fa بررسی اثر موضعی پماد هیدرو الکلی پوست پسته بر روند التیام زخم در موش صحرایی نر: یک مطالعه بافت فارماکولوژی Pharmacology پژوهشي اصیل Original Research <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">زمینه و هدف:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> با توجه به اینکه مغز و پوست پسته دارای اثر ضد التهابی، ضد میکروبی و آنتی اکسیدانی است هدف از انجام این مطالعه بررسی اثرات پماد عصاره هیدرو الکلی پوست پسته بر روند التیام زخم است.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">مواد و روش&shy;‌ها:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> جهت ارزیابی پلانی متری 20 سر رت به‌طور تصادفی به 5 گروه 4 تایی: گروه کنترل، گروه شاهد (زخم باز همراه با پماد پایه بدون عصاره)، گروه یک</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">درصد، گروه دو درصد و گروه چهار درصد تقسیم بندی </span></span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">شدند.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> زخم&shy;ها به مدت 10 روز با نرمال سالین شستشو و در گروه های درمان پماد تجویز گردید.</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">در روز‌های 7 و 14 جهت ارزیابی هیستوپاتولوژی، بافت پوست محل زخم نمونه&shy;برداری شده و</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">جهت ارزیابی کلاژن، رنگ آمیزی ماسون تری کروم صورت پذیرفت.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">یافته&shy;ها:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> بررسی هیستوپاتولوژی لام گروه‌های زخم نشان داد که پماد هیدرو الکلی پوست پسته در غلظت&shy;های متفاوت منجر به افزایش معنی&shy;داری</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">در درصد بهبود زخم، افزایش ضخامت پوست، قطر کلاژن رسوب&shy;گذاری شده و کاهش ادم در مقایسه با گروه&shy;های کنترل گردیده است. همچنین سلول&shy;های التهابی در روز 14 در گروه&shy;های درمان شده با عصاره پوست پسته</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">در مقایسه با گروه کنترل کاهش معناداری </span></span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">نشان داد که</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">بیانگر تسریع روند بهبود زخم<strong> </strong>در نمونه‌های تحت تیمار است</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">.(p<0.05)</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">نتیجه</span></span></b><b><span dir="LTR" style="font-size:12.0pt">&shy;</span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">گیری:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> با توجه به نتایج حاصل از این پژوهش، پماد حاوی عصاره پسته می</span></span><span dir="LTR" style="font-size:12.0pt">&shy;</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">تواند تأثیرات</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">التیام</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">دهنده</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">در</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">زخم</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">ایجاد</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">شده</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">در</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">پوست</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">رت</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">داشته</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">باشد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span></span></span></span></span><br> <br> <br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background and Aim:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Pistachio nut and hull have anti-inflammatory, antimicrobial and antioxidant effects. The aim of this study was to investigate the effects of pistachio hull hydroalcoholic extract ointment on wound healing process.</span></span><b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> For wound planimetric assessment, 20 healthy rats were randomly divided into 5 groups of 4, including control group (open wound without any intervention and treatment), positive control group (open wound with basic ointment without extract), and the 3 other groups receiving 1%, 2% and 4% ointments respectively. On the 7^th&nbsp; and 14^th days, biopsies from wound tissues were taken and after Mason Trichrome staining we evaluated collagen. H&E staining was done for histopathological assessments.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The results showed that wound treatment with pistachio hull ointment in the experimental groups showed a significant increase (P<0.05) in the percentage of wound healing rate. Also, there was a significant difference in the thickness of skin and collagen diameter, and reduction of edema between experimental and control groups (<i>P</i><0.05). Also we found significant decrease in inflammatory cell count on the 14^th day in the groups treated with pistachio hull extract compared to those in the control group (<i>P</i><0.05), which was indicative of accelerated wound healing in the experimental groups.<b> </b></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">: According to the results of this study, </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">pistachio hull hydroalcoholic extract ointment</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> potentially possesses healing effects on the wound created in the skin of the rat.&nbsp;</span></span></span></span></span></span></span><br> &nbsp; پماد, گیاهان دارویی, عصاره گیاه, رنگ آمیزی Ointments, Medicinal plants, Plant extracts, Staining 12 22 http://sjku.muk.ac.ir/browse.php?a_code=A-10-1815-3&slc_lang=fa&sid=1 Masoumeh Pourjabali معصومه پورجبلی pourjabali.M@umsu.ac.ir 5400319475328460051394 5400319475328460051394 No Assistant Professor, Department of Pathology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran استادیار، گروه پاتولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی ارومیه، ارومیه ، ایران، Leila Zarei لیلا زارعی leilazarei652@yahoo.com 5400319475328460051395 5400319475328460051395 Yes Associate Professor, Department of Anatomical Sciences, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. دانشیار، گروه علوم تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی لرستان، خرم آباد، ایران،) Vahid Ezzati Sarai وحید عزتی سرای vahidezzati@gmail.com 5400319475328460051396 5400319475328460051396 No Medical Student, Student Research Committee, Urmia University of Medical Sciences, Urmia, Iran دانشجوی دکتری عمومی، دانشکده پزشکی، دانشگاه علوم پزشکی ارومیه، ارومیه ، ایران