Scientific Journal of Kurdistan University of Medical Sciences

fa بررسی ارتباط پلی مورفیسم INDEL HLA-G با بیماری سیستمیک لوپوس اریتماتوز(SLE) در استان گیلان Association of HLA-G INDEL polymorphism with systemic lupus erythematosus (SLE) in Guilan Province, in Iran عمومى General پژوهشي اصیل Original Research زمینه و هدف سیستمیک لوپوس اریتماتوز (SLEعلت تاکنون ناشناخته است اما برخی<span b="" dir="LTR" font-size:="" lang="AR-SA" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" simplified="" span="" style="font-family: " times=""> نقش دارند. کلاس I ی<span .="" b="" dir="LTR" face="Calibri" font="" font-size:="" lang="AR-SA" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-hansi-font-family:="" new="" simplified="" span="" style="font-family: " times=""> به خانواده آنتیژنهای غیر کلاسیک HLA تعلق دارد.  هدف از این مطالعه بررسی ارتباط پلیمورفیسم INDEL (درج یا حذف قطعه bp14) ناحیه  3'UTR ژن HLA-G در بیماران مبتلا به سیستمیک لوپوس اریتماتوز می باشد. در این تحقیق 80 بیمار مبتلا به SLE ژنومی از خون محیطی استخراج گردید. تعیین ژنوتیپ توسط ARMS-PCR (ویرایش ) صورت گرفت. <span :="" b="" color="#000000" dir="LTR" font="" font-size:="" lang="AR-SA" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" span="" style="color: black; font-family: " times="">14 (bp<span b="" dir="LTR" font-size:="" lang="AR-SA" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" span="" style="color: black; font-family: " times="">14+)، هتروزیگوتها (bp<span b="" dir="LTR" font-size:="" lang="AR-SA" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" span="" style="color: black; font-family: " times="">14-) و هموزیگوتهای حذف قطعه bp<span 14="" b="" dir="LTR" font-size:="" lang="AR-SA" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" span="" style="color: black; font-family: " times="">14-/bp<span .="" b="" dir="LTR" font-size:="" lang="AR-SA" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" span="" style="color: black; font-family: " times="">14+/bp<span .="" b="" dir="LTR" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" span="" style="color: black; font-family: " times="">05/0<P). پلیمورفیسم INDEL <span b="" dir="LTR" font-size:="" lang="AR-SA" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" simplified="" span="" style="font-family: " times=""> مرتبط نیست. <span b="" dir="LTR" font-size:="" font-style:="" lang="AR-SA" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times=""> در بیمار SLE<span a="" b="" color="#000000" dir="LTR" font="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-hansi-font-family:="" name="OLE_LINK3" new="" p="" span="" style="font-family: " times="">، 14bp وصول مقاله:21/4/94 اصلاحیه نهایی:19/8/94 پذیرش:2/9/94 Background and Aim: Systemic lupus erythematosus (SLE) is an autoimmune disease that is characterized by the presence of autoantibodies against nuclear antigens. The exact cause of SLE is unknown, but some of these genes are active in the key pathways, including immune complexes, host immune signal transduction, and interferon pathways which have significant roles in the pathogenesis of SLE. Since the classical HLA class I and II molecules are highly involved in the peptide presentation to the components of immune system, the genes that encode these molecules can be primary candidates associated with susceptibility to autoimmune disorders. HLA-G belongs to the family of non-classical HLA class I antigens. The purpose of this study was to evaluate the association of INDEL polymorphism of the 3’UTR region of HLA-G gene with SLE. Material and Methods: In this study, 80 patients with SLE, and 102 healthy individuals were examined. Genomic DNA was extracted from peripheral blood. The genotypes were determined using ARMS-PCR. Data analysis was performed using MedCalc version 12.1. Results: The frequencies of homozygot genotypes for the presence of (+14bp/+14bp)14bp, hetrozygot genotypes for (-14bp/+14bp), and homozygot genotypes with deletion of (-14bp/-14bp)14bp segments in the healthy individuals were  21.57%, 41.18% and 37.25%, and in the patients were 17.5%, 42.5% and 40% respectively. The most frequent genotype in the healthy individuals and hetrozygot patients was (-14bp/+14bp). We found no statistically significant differences in the genotype distributions between the cases and controls (Ρ>0.05). Conclusion: In this study INDEL polymorphism of the 3’UTR region of HLA-G gene showed no association with systemic lupus erythematosus. However, further studies are required to confirm the validity of these results. Keywords: Systemic lupus erythematosus, HLA-G gene, INDEL polymorphism, 14bp   Received: Jul 12, 2015      Accepted: Nov 23, 2015 سیستمیک لوپوس اریتماتوز, ژن HLA-G , پلی مورفیسم INDEL, 14bp Systemic lupus erythematosus, HLA-G gene, INDEL polymorphism, 14bp 44 53 http://sjku.muk.ac.ir/browse.php?a_code=A-10-25-42&slc_lang=fa&sid=1 Leila Asali Salek Moalemi لیلا عسلی سالک معلمی 5400319475328460018721 5400319475328460018721 No Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران. Fatemeh Asali Salek Moalemi فاطمه عسلی سالک معلمی 5400319475328460018722 5400319475328460018722 No Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران. Zivar Salehi زیور صالحی geneticzs@yahoo.co.uk 5400319475328460018723 5400319475328460018723 Yes Department of Biology, Faculty of Sciences, University of Guilan گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران Seyed Habib Zayeni سید حبیب زینی 5400319475328460018724 5400319475328460018724 No گروه بیماری های داخلی، دانشکده پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران.