:: Scientific Journal of Kurdistan University of Medical Sciences - No 2, 2022 ::
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The effect of Vaspin on Fatty acids metabolism in steatosis model: comparison between two-dimensional (2D) and three-dimensional (3D) culture
Alina Abdollahi1 , Esmael Izadpanah2 , Zakaria Vahabzadeh3 , MOHAMMAD RAMAN MOLOUDI 4
1- Ph.D. Candidate of Molecular Medicine, Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
2- Assistant Professor of Physiology, Liver & Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
3- Assistant Professor of biochemistry, Liver & Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
4- Kurdistan University of Medical Sciences , x.moloudi@gmail.com
Abstract:   (247 Views)
Background and Aim: Non-alcoholic fatty liver disease, characterized by abnormal fat accumulation in the liver, is associated with obesity and insulin resistance. Vaspin is an adipokine secreted by adipose tissue, and its gene expression is increased when insulin sensitivity is reduced. In this study, the effect of vaspin on fatty acid metabolism in a 3D and 2D culture was investigated.
Materials and Methods: The steatosis model was induced by oleic and palmitic acid in HepG2 cell line in two- and three-dimensional culture (collagen gel). The cells were then treated with 100 ng/ml vaspin for 24 hours. Expression of FABP4, MCAD, FAS and ApoB100 genes was measured by Real time PCR.
Results: In two- and three-dimensional cultures, the expression of FABP4 and FAS genes showed a significant decrease. There was no significant difference between the expressions of these genes in two- and three-dimensional culture. Expression of MCAD and ApoB100 genes was significantly increased in 2D and 3D cultures. MCAD and ApoB100 gene expression was not significantly different in two- and three-dimensional cultures.
Conclusion: The results of this study showed that vaspin treatment can alter the expression of genes involved in uptake, lipogenesis, oxidation and secretion of fatty acids compared to the control group in 2-D and 3-D culture. While the difference in expression of these genes in 2-D culture compared to 3-D was not significant.
Keywords: Collagen, Hep G2 cells, non-alcoholic fatty liver disease, lipogenesis
     
Type of Study: Original Research | Subject: Physiology and neurosciences
Received: 2021/04/24 | Accepted: 2021/08/3

Ethics code: IR.MUK.REC.1397/46


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