:: Scientific Journal of Kurdistan University of Medical Sciences - No 2, 2022 ::
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HPLC determination of major active components in the Iranian (Kurdistan) propolis extract
Vahid Yousefinejad1 , Nazila Darvishi2 , Zakaria Vahabzadeh1 , Asrin Babahajian3 , Sayed Hossein Davoodi 4
1- Assistant Professor, liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
2- PhD of Nutrition, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3- MSc of Anatomical Science, liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
4- Professor, Nutrition Department, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , hdavoodi1345@gmail.com
Abstract:   (247 Views)
Background and Aim: Propolis, as a natural product, has been shown to provide several biological benefits including antioxidant, anti-inflammatory, and antimicrobial as well as anticancer properties. Its application extensively depends on its composition and the amounts of the major active components. Therefore, in the presented study we aimed to evaluate the amounts of chrysin, quercetin and, caffeic acid as three active components, in the Iranian propolis using UV-HPLC method.
Materials and Methods: Three different samples of propolis were collected from Saral & Avalan regions (Kurdistan Province, Iran) during spring 2017. To separate and measure the three desired components, the samples were then extracted with an ethanolic solution (80%) and subsequently injected to the UV-HPLC system with an isocratic mode at a flow rate of 1ml/min for 20 min and ultraviolet detection at λmax 260 or 310 nm. The linearity and detection limits of the method were calculated to evaluate the quality control of the assay.
Results: Chrysin was not detected in our samples. The average amounts of quercetin and caffeic acid were 65.425 and 27.498 µg/g, respectively.
Conclusion: The results of this study, for the first time, accurately quantified the amounts of three active components of Iranian propolis which many of propolis's beneficial effects are attributed to them
Keywords: Propolis, Chrysin, Quercetin, Caffeic acid, HPLC, Iran.
     
Type of Study: Original Research | Subject: Biochemistry and Clinical Laboratory
Received: 2021/04/3 | Accepted: 2021/09/13


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