per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
1
8
article
Survival rate estimation and its associated factors in patients with stomach cancer in Tohid Hospital in Sanandaj
Yousef Veisani
1
Ali Delpisheh
alidelpisheh@yahoo.com
2
Koroush Sayehmiri
3
Ezatollah Rahimi
4
Ilam University of Medical Sciences
Ilam University of Medical Sciences
Ilam University of Medical Sciences
Kurdistan University of Medical Sciences
ABSTRACT
Background and Aim: Estimation of the survival rate after diagnosis is an important indicator of treatment process in cancer patients. The aim of the present study was to determine 5-yearsurvival rate and its associated factors in the patients with stomach cancer.
Materials and Methods: This was a descriptive analytical study. We reviewed records of 239 patients with confirmed diagnosis of stomach cancer who had been hospitalized at Tohid Hospital in Sanandaj city, during a five-year interval from 2006 onwards. Survival rate was calculated according to the Kaplan-Meier and by using Log rank statistical methods.
Result: Our study included 178 male (74.5%) and 61 female (25.5%) patients with a mean age of 68.8 ±11.97 years. The mean maximum survival rate was 19.85 months (CI 95%: 16.46-23.24) which belonged to the patients with intestinal type of tumors (60.6%, n=145), followed by the patients who had surgical therapy (10.3%, n=21) with a mean survival rate of 15.67 months (CI95%: 8.78-22.58). Variables such as gender, residential areas, and history of stomach cancer in first degree relatives, history of symptoms of gastric diseases, cigarette smoking and psychiatric disorders during treatment course showed no significant association with mean survival rate. The mean survival rate in the patients which their cancer had been diagnosed at early stages was 14.86 months (CI 95%, 12.77-16.94) which was significantly higher as compared to those with late diagnosis of the disease(P=0.049). One to five year survival rates were 41%, 17%, 13%, 10% and 5.4% respectively.
Conclusions: Early diagnosis of stomach cancer increases 5 years survival rate in the patients significantly.
Conflict of Interest: Nil
Received: Mar7, 2012 Accepted: Jan23, 2013
http://sjku.muk.ac.ir/article-1-1060-en.pdf
Stomach cancer
Survival rate
Sanandaj.
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
9
17
article
Assessment of Illness perception in the patients with diabetes mellitus and its association with control of blood sugar in the patients referring to Tohid Hospital in Sanandaj city
Fahimeh Valipoor
1
Farzin Rezaei
frrezaie@yahoo.com
2
Kurdistan University of Medical Sciences
Kurdistan University of Medical Sciences
ABSTRACT
Background and Aim: Illness perception affects patients' behavior, compliance, self-management and consequences of the disease. The aim of this study was to determine the illness perception and its relation to treatment response in the patients with diabetes mellitus in Tohid Hospital.
Material and Methods: In this descriptive-analytical study, 200 diabetic patients were randomly selected. The illness perception data were obtained using IPQ questionnaire, and other variables including HbA1C were extracted from the patients' records. Collected data were entered into SPSS software and analyzed by means of Mann-Whitney U and Spearman correlation coefficient statistical tests. p
http://sjku.muk.ac.ir/article-1-1061-en.pdf
Illness perception
Diabetes
Blood sugar control
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
18
25
article
One novel PMS2 germline mutation in a patient with hereditary non polyposis colorectal cancer
Mahdi Montazer Haghighi
mah_haghighi@hotmail.com
1
Mohammad Yaghoob Taleghani
2
Zahra Akbari
3
Maedeh Alidadi
4
Ehsan Nazemalhosseini Mojarad
5
Mahsa Molaei
6
Mohammad Reza Zali
7
Shahid Beheshti University of Medical Sciences
Shahid Beheshti University of Medical Sciences
Shahid Beheshti University of Medical Sciences
Shahid Beheshti University of Medical Sciences
Shahid Beheshti University of Medical Sciences
Shahid Beheshti University of Medical Sciences
Shahid Beheshti University of Medical Sciences
ABSTRACT
Background and Aim: Hereditary non polyposis colorectal cancer (HNPCC) is an autosomal dominant syndrome. The cancer appears between 40 and 50 years of age. Mutation in mismatch repair genes can lead to this cancer .One of the genes which is involved in this disease is PMS2 gene. Here, we present a case with a novel germline mutation in PMS2 gene. The aim of this report was to examine PMS2 gene and identify novel germline mutations in this gene.
Case presentation: A 77-year-old male with diagnosis of colorectal carcinoma was referred for genetic testing. He suffered from a polyp with a diameter of 6.8 cm in hepatic flexure. The patient did not meet Amsterdam Criteria and Bethesda Guidelines, but screening for HNPCC was performed on account of pathological findings. Blood sample was used for identification of mutation and the paraffin embedded block was prepared for MSI analysis.
Conclusion: One mutation in PMS2 gene was detected by analysis of the amplicon sequencing. The mutation was a transitional mutation in position 676 which led to transformation of guanine to adenine resulting in substitution of glutamic acid for glycine. Immunohistochemistry confirmed abnormal expression of PMS2 gene and MSI assay showed instability of sequenced amplicons in this gene.
Conflict of Interest: Nil
Received: Mar 11, 2012 Accepted: Apr 7, 2013
http://sjku.muk.ac.ir/article-1-1062-en.pdf
HNPCC
PMS2 gene
MSI.
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
26
35
article
The effect of sesame oil on the liver phosphatidate phosphohydrolase and serum lipoproteins in hypercholesterolemic rabbits
Esfandiar Heidarian
heidarian_e@skums.ac.ir
1
Baharak Kashani
2
Mahmood Rafieian-Kopaei
3
Reza Hajhosseini
4
Roya Ansari-Samani
5
Shahrekord University of Medical Sciences
Tehran Payamenour University
Shahrekord University of Medical Sciences
Tehran Payamenour University
Shahrekord University of Medical Sciences
ABSTRACT
Background and Aim: Nowadays, the effect of medicinal plants on the reduction of the prevalence of cardiovascular diseases and atherosclerosis has been confirmed. Liver phosphatidate phosphohydrolase (PAP) is a key regulatory enzyme in the glycerolipid metabolism. The aim of this study was to assess the effects of sesame oil on liver PAP activity, liver triglyceride, liver cholesterol and serum lipoprotein levels in hypercholesterolemic rabbits.
Material and Methods: In this experimental study 27 New Zealand rabbits were randomly assigned to 3 groups (n=9). Group1 (control) was fed with standard diet. Group II (hypercholesterolemic group) animals received hypercholesterolemic diet (1%) without treatment. Group III was fed with hypercholesterolemic diet (1%) plus sesame oil (5%). After two months, liver PAP activity, liver triglyceride and cholesterol content, serum lipoproteins and malondialdehyde, and antioxidant capacity were measured. One way ANOVA was used for analysis of the mean values of the variables and for pair-wise comparison of the groups we used Tukey’s test.
Results: Group III had a significant decrease (P< 0.05) in the liver PAP activity compared to group II. In group II, consumption of the enriched cholesterol diet led to a significant elevation (P< 0.05) in serum lipoproteins compared to group I (control). Also, sesame oil in group III decreased the serum lipoproteins, liver triglyceride, and liver cholesterol in comparison to group II (p<0.05). However, a significant elevation (P< 0.05) in serum antioxidant capacity and a significant reduction in malondialdehyde level occurred in group III compared to group II (P<0.05).
Conclusion: Sesame oil can be effective in reducing risk factors of cardiovascular diseases by decreasing serum lipids through making desirable alterations in serum lipoproteins. Also addition of sesame oil to hypercholesterolemic diets can reduce the liver PAP activity resulting in reduced liver triglyceride synthesis, which can decrease the risk of development of fatty liver in hypercholesterolemic diets.
Conflict of Interest: Nil
Received: Feb 25, 2012 Accepted: Feb 6, 2013
http://sjku.muk.ac.ir/article-1-1063-en.pdf
Sesame oil
Phosphatidate phosphohydrolase
Hypercholesterolemia
Liver triglyceride
Serum lipoproteins.
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
36
43
article
Comparison of oral and intravenous N-acetylcysteine administration in the treatment of acetaminophen poisoning
Mohammad Arefi
1
Behnam Behnoush
2
Hamed Pouraziz
hamedpk53@yahoo.com
3
Vahid Yousefinejad
4
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
ABSTRACT
Background and Aim: Intravenous or oral administration of N-acetylcysteine for acetaminophen poisoning has been a matter of controversy until recently. Each of these routes of administration has its advantages and disadvantages. This study was carried out to compare the effect of intravenous and oral acetylcysteine in the treatment of acetaminophen poisoning.
Material and Methods: This study was a parallel RCT study. The study population included all adults over 18 years of age who had been used more than 7.5 gr acetaminophen during a 24 hour interval before admission to poisoning emergency ward of Baharloo Hospital in Tehran. Each group consisted of 30 patients. The study was approved by the Ethical Committee of Tehran University of Medical Sciences. Patients were divided into two groups by block randomization method: oral (72 h) and IV (20 h) N-acetylcysteine treatment groups. Liver enzyme levels were measured at baseline and then daily up to 72 hours after treatment. Side effects of drug administration in both groups were recorded.
Data were entered into SPSS16 software and the data frequency tables were prepared. Chi-square, Mann-Whitney U and t-tests were used for data analysis.
Results: There were no statistically significant differences between the two groups in relation to different variables at baseline. The two groups showed no significant differences in regard to the mean serum levels of AST, ALT, bilirubin, and prothrombin time24, 48 and 72 hours after treatment and the therapeutic results were similar in both groups.
Occurrence of nausea and hypotension after treatment in the oral treatment group was significantly higher than in the intravenous treatment group (57.6٪ vs. 33.3٪ and 12.1٪ vs. Zero respectively) (p<0.05). Considering other complications, no significant differences were noticed between the two groups.
Conclusion: Considering the same safety and efficacy of both intravenous and oral acetylcysteine treatment, mild and manageable side effects of oral therapy on one hand and economic status and cultural attitudes in our community about admission and discharge of the patients, it seems more advisable to use oral treatment as the first therapeutic choice for acetaminophen poisoning in the absence of any contraindication to its use.
Irct ID: IRCT201112218488N1
Conflict of Interest: Nil
Received: Feb25, 2013Accepted: Apr24, 2013
http://sjku.muk.ac.ir/article-1-1064-en.pdf
Acetaminophen
N-Acetylcysteine
Poisoning
Drug administration method
Complications.
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
44
50
article
Correlation between iron deficiency anemia and febrile convulsion
Parsa Yousefi
1
Aziz Eghbali
2
Mohammad Rafeie
3
Mohaddeseh Zolfi
dr.firouzifar@arakmu.ac.ir
4
Mohammad Reza Firouzifar
5
Arak University of Medical Sciences
Arak University of Medical Sciences
Arak University of Medical Sciences
Arak University of Medical Sciences
Arak University of Medical Sciences
ABSTRACT
Background and Aim: Febrile convulsion is the most common neurological disease of childhood. Regarding the current hypotheses about possible effect of iron deficiency on prevention of febrile convulsion and its relationship to neuronal stimulation threshold, we conducted this study to evaluate the relationship between iron deficiency anemia and simple febrile convulsion.
Materials and methods: In this case-control study, 382 children who met our inclusion criteria and had been admitted in Arak Amirkabir Hospital were assigned to two groups: case (with febrile convulsion) and control (other febrile diseases). After the fever subsided, a 5 cc sample of blood was taken from every patient for complete blood count and iron reserve profile. The results were analyzed by using descriptive statistics and independent t-test.
Results: Iron deficiency anemia was significantly less frequent in our case group compared to the control group (22.5% vs. 34.0%) (p<0.001). CBC indices such as Hb, HCT, MCV, MCH and MCHC were significantly (p<0.001) higher in the case group in comparison to the control group.
Conclusion: Iron deficiency can prevent the febrile convulsion probably by increasing neuronal excitation threshold.
Conflict of Interest: Nil
Received: May 16, 2012 Accepted: Apr7, 2013
http://sjku.muk.ac.ir/article-1-1065-en.pdf
Febrile convulsion
Iron deficiency anemia
Children
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
51
57
article
Prevalence of human T-lymphotropic virus types I;II among high risk groups in Sanandaj in 2010
Naaman Ardalan
1
Mohammad Abdi
2
Bahareh Rahimian Zarif
3
Amir Amini
4
Farzam Meamari
5
Erfan Haydari
6
Abbas Ahmadi
abbasahmady@yahoo.com
7
Islamic Azad University of Sanandaj
Tarbiat Modarres University
Islamic Azad University of Sanandaj
Baqiyatallah University of Medical Sciences
Islamic Azad University of Sanandaj
Islamic Azad University of Sanandaj
Kurdistan University of Medical Sciences
ABSTRACT
Background and Aim: HTLV-I is the etiological cause of adult T-lymphocytic leukemia (ATL) and a chronic degenerative neurologic disorder, called tropical spastic paraparesis (TSP). HTLV infection can be transmitted through different ways: from mother to child or fetus, sexual intercourse, transfusion of contaminated blood or blood products, and sharing contaminated syringe needles. As the presence of these infections in high risk groups can be an approximate indicator of their prevalence in the society and blood donors, in this study was tried to find prevalence of HTLV in HIV positive or negative intravenous drug users (IVDU patients with major thalassemia, and hemodialysis patients, in Sanandaj.
Materials and Methods: This descriptive study included 351 cases: 130 HIV positive and 110 HIV negative intravenous drug users (IVDU), 46 cases of major thalassemia, and 65 hemodialysis patients. All participants completed written informed consent forms. After obtaining blood samples and serum separation, all specimens were kept in freezer at -20oC up to the time of analysis. Serum samples were screened for measurement of the titers of HTLV I;II antibodies by Dia-Pro ELISA kits, manufactured in Italy. Positive and suspicious reactions were reanalyzed. For confirmation of positive and suspicious reactions, samples with one positive reaction were examined by use of western blot kid (HTLV blot 2.4, manufactured by MP Diagnostics in Switzerland). Data were entered into SPSS 16 software and the prevalence rates of these viruses were obtained by using frequency distribution table.
Results: The results of this study showed that one HIV positive, one HIV negative patient and another patient with major thalassemia were HTLV I positive (0.85%). None of the hemodialysis patients had antibody against HTLV type 1. We did not find any antibody against HTLV type 2 in our study.
Conclusions: The prevalence rate of HTLV (types 1 and 2) among these high risk groups was not high in Sanandaj. This may reflect its low prevalence in general population and in blood donors. However, it is necessary to take preventive measures to reduce its spread. To assess the exact prevalence rate we recommend screening of all donated blood samples and general population.
Conflict of Interest: Nil
Received: May 24, 2012 Accepted: Oct 11, 2012
http://sjku.muk.ac.ir/article-1-1066-en.pdf
HIV
HTLV I&II
Major thalassemia
Intravenous drug user
Hemodialysis
ELISA
Western Blot
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
58
66
article
Role of pain and its associated cognitive related variables in the patients with rheumatoid arthritis
Samaneh Ghooshchian Choobmasjedi
samaneh_641@yahoo.com
1
Jamileh Moghimi
2
Behnaz Behnam
3
University of Tarbiat Moallem
Semnan University of Medical Sciences
Semnan University of Medical Sciences
ABSTRACT
Background and Aim: In recent decades there had been considerable progress in the research works on chronic pain, perhaps as a consequence of costs and challenges associated with this problem. The aim of this study was to compare rheumatoid arthritis patients and healthy individuals in regard to fear of pain, pain catastrophizing and catastrophic cognition.
Materials and methods: In this study we selected 100 patients with rheumatoid arthritis and 100 healthy individuals from a public hospital using census sampling method. After informed consent, demographic variables such as age, sex, level of education, duration of illness were recorded for every subject. We used three questionnaires of fear of pain (FPQ), catastrophic cognition (CCQ) and scale of pain catastrophizing (PCS) in this study.
Results: Multivariate analyses of variance (MANOVA) showed significant differences between the two groups in regard to the three cognitive variables of "fear of pain", "catastrophic cognition" and "pain catastrophizing"(P<0.05).
Conclusion: These results suggested that fear of pain catastrophic cognition and pain catastrophizing were important cognitive factors contributing to aggravation of pain in the patients with rheumatoid arthritis. The results of the current study are consistent with basic assumptions of cognitive–affective model which emphasizes the importance of the ‘pain experience’ as potential modulators of the pain–cognition relationship.
Conflict of Interest: Nil
Received: Feb 8, 2012 Accepted: Apr 7, 2013
http://sjku.muk.ac.ir/article-1-1067-en.pdf
Fear of pain
Pain catastrophizing
Catastrophic cognition
Rheumatoid arthritis.
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
67
76
article
The effect of genu varum deformity on balance control following postural perturbation in adolescent girls
Maryam Panah-abadi
1
Azar Aghayari
2
Fatemeh Salari Esker
3
Mehrdad Anbarian
m_anbarian@yahoo.com
4
Payame Noor University, Tehran,
Payame Noor University, Tehran,
Bu Ali Sina University
ABSTRACT
Background and Aim: Despite the fact that balances has often been considered as a measure of lower extremity function, the effect of lower extremity malalignment on balance performance has received little attention. The aim of the present study was to examine postural control following a sudden external perturbation in adolescent girls with genu varum of the knees in comparison to age matched healthy controls.
Materials and methods: This comparative and interventional study included eight adolescent girls with genu varum (age: 13. 5± 1.4 years) and eight age-matched healthy controls (age: 13.8 ± 1.3). The subjects underwent unexpected forward, backward and medio-lateral perturbations triggered by a weight equivalent to 10% of the body weight, of every subject while standing on a foot pressure system mounted on a movable plate. The displacement of the center of pressure (COP) of the feet was measured. Independent t-test was used for statistical analysis. p≤0.05 was considered significant.
Results: Subjects with genu varum of the knees showed a significantly greater COP displacement after perturbation in comparison to the control group (p=0.039).
Conclusion: Our findings revealed that postural control following balance perturbations is weaker in subjects with genu varum deformity than in control group. Consequently, abnormal lower limb alignment is a risk factor which can influence postural control in response to a balance perturbation.
Conflict of Interest: Nil
Received: Jan 17, 2012 Accepted: Dec 20, 2012
http://sjku.muk.ac.ir/article-1-1068-en.pdf
Balance perturbation
Genu varum
Adolescent girls
Postural control.
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
77
85
article
Molecular typing of Salmonella enteritidis strains isolated in several laboratory centers in Tehran by ERIC-PCR
Reza Ranjbar
ranjbarre@gmail.com
1
Reza Torabi
2
Amir Mirzaie
3
Baqiyatallah University of Medical Sciences
Baqiyatallah University of Medical Sciences
Baqiyatallah University of Medical Sciences
ABSTRACT
Background and Aim: Salmonella enterica subspecies enterica serotype enteritidis is one of the most frequent serotypes that cause gastroenteritis and bacteremia in human. The aim of the present study was to study the molecular types of Salmonella enteritidis strains isolated from different laboratory centers in Tehran by ERIC-PCR.
Materials and methods: In this descriptive study from April 2008 to December 2011, Salmonella isolates were obtained from the patients admitted to several hospitals in Tehran. Salmonella isolates were identified by the standard microbiological methods and were serotyped by slide agglutination with commercial mono and polyvalent antisera. Molecular typing of the strains and their genetic relationship were investigated by using ERIC-PCR.
Results: All Salmonella enteritidis strains investigated by ERIC-PCR were type able. This technique produced 6 to 17 amplified DNA bands with 200 to 3200 different bp. In general, ERIC-PCR differentiated all Salmonella enterica serotype enteritidis isolates into nine distinct clusters (E1-E9), however the majority of the strains (35%) belonged to cluster E1.
Conclusion: In this study the most common serotype was Salmonella enteritidis and ERIC-PCR showed that the strains belonged to diverse genotypic clusters.
Conflict of Interest: Nil
Received: Feb8, 2012 Accepted: Mar11, 2013
http://sjku.muk.ac.ir/article-1-1069-en.pdf
Salmonella enteritidis
ERIC-PCR
Genotyping
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
86
96
article
Effect of upper and lower body exercises on cardiovascular, metabolic and hematologic factors
Fariborz Havanloo
1
Sajjad Ahmadizad
2
Ameneh Mardani
3
Mina Sahami
mina.sahami@gmail.com
4
Shahid Beheshti University
Shahid Beheshti University
Shahid Beheshti University
Shahid Beheshti University
ABSTRACT
Background and Aim: Considering the sophisticated responses of cardiovascular system to different types of body exercises, dealing with this system and the effect of exercise on it has assumed an added importance. The aim of this study was to compare the effect of two types of exercise, arm cranking and leg cycling, on cardiovascular, metabolic and hematologic factors.
Material and Methods: After obtaining informed consent 10 non athlete female students with a mean age of 25±2.69 years and mean height of 161.75±5.5cm participated in this study. They completed four experimental testing sessions (two maximal oxygen consumption test on a cycle ergometer and arm cranking to assess variations in respiratory gases and heart rate by using ergometer cycling and arm cranking vo2max, and two sub-maximal exercises including warm up for 5 minutes and body exercise on cycle ergometer or arm cranking with 70% of maximum O2 consumption or 75-80% of maximum heart rate, for 30 minutes. Then the subjects took a 30 min rest in the sitting position. Heights and weights of the subjects were measured before exercise. Before and after exercise and after 30 min rest in sitting position, heart rate and blood pressure were recorded and blood samples were collected for measurement of blood lactate concentration, RBC, Hb, Hct, MCV, MCH and MCHC.
Results: No differences were found between the two types of exercises in regard to the HR, RBC, Lac, HG, Hct, MCV and MCHC but there were significant differences in relation to BP and MCH (P=0.013 and f1.9 =9.385 for blood pressure P=0.046 and f1.9=5.372 for MCH).
Conclusion: The results indicated no differences in the cardiologic, hematologic and metabolic factors during exercises with similar relative intensity. But we found a higher BP after arm cranking and an increase in MCH after leg exercise. Also, there were significant differences in all parameters except HG and MCH, before and after exercise.
Conflict of Interest: Nil
Received: Apr 4, 2012 Accepted: Dec 5, 2012
http://sjku.muk.ac.ir/article-1-1070-en.pdf
Arm cranking
Ergometer cycling
Cardiovascular factors
Metabolic and hematologic factors.
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
97
105
article
Isolation and assessment of phenotypic and genotypic characteristics of metallo-β-lactamase genes in Pseudomonas aueroginosa strains isolated from clinical samples at Vali-e-Asr Hospital in Zanjan province
Masoomeh Doosti
1
Ali Ramazani
ramazania@zums.ac.ir
2
Mahdi Hajojagh Faghihi
3
Zanjan University of Medical Sciences
Zanjan University of Medical Sciences
Zanjan University of Medical Sciences
ABSTRACT
Background and Aim: Infections caused by Pseudomonas aeruginosa and dissemination of metallo-beta-lactamases (MBL) have been reported with increasing frequency through-out the world. The aim of this study was to investigate the antibiotic susceptibility and identification of blaVIM and blaIMP genes in P. aeruginosa isolates in Zanjan Province.
Material and Methods: A total of 70 P. aeruginosa isolates were identified from the patients at intensive care units (ICU) in Zanjan Province. The antimicrobial susceptibility was tested by disk diffusion (Kirby-Bauer) method and MBL producing strains were identified by double-disk synergy test (DDST). From genotypical point of view the presence of blaVIM and blaIMP genes and class 1 integron was confirmed by PCR.
Results: Of 70 strains of P. aeruginosa detected by phenotypic method, the highest rate of resistance was found in the following order: meropenem, cefotaxime and ceftazidime, imepenem gentamicin, piperacillin, and ciprofloxacin.DDS test showed that of 44 resistant isolates to imepenem 36 (81.8%) were MBL producing. We found 33 MBL producing strains by PCR, 23 isolates had VIM gene (52.2%) and 10 isolates carried IMP gene (22.3%). The IMP gene in 8 of the 10 isolates was IMP1 (18.2%). Of 44 resistant strains to imepenem only 31 isolates (70.5%) had class 1 integron gene.
Conclusion: Our results showed that prevalence of antibiotic resistance due to MBL production in our province is on the rise and among the MBL producing strains the frequency of VIM genes is higher than IMP genes.
Conflict of Interest: Nil
Received: Apr 4, 2012 Accepted: Apr 7, 2013
http://sjku.muk.ac.ir/article-1-1071-en.pdf
Pseudomonas aeruginosa
Metallo-beta-lactamases
Class 1 integron
PCR
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
106
113
article
Descriptive investigation of causes of thunderclap headache in patients at Imam-Reza Hospital in Kermanshah from 2009 to 2011
Nazanin Razazian
nrazazian@gmaill.com
1
Maryam Baziar
2
Mahnaz Tavakolian
3
Sasan BahramiGhiasabadi
4
Mohammad Hadadian
5
Kermanshah University of Medical Sciences
Kermanshah University of Medical Sciences
Kermanshah University of Medical Sciences
Kermanshah University of Medical Sciences
Kermanshah University of Medical Sciences
ABSTRACT
Background and Aim: Headache is one of the most frequent causes of patient attendance in emergency rooms. One important type of headache is known as thunderclap headache which reaches its peak in approximately one minute. It has several different causes.
The aim of this study was to evaluate the demographic and clinical characteristics and causes of this type of headache in the patients referring to Imam-Reza hospital in Kermanshah.
Material and Methods: In this descriptive analytic study, we reviewed the records of all the patients with thunderclap headache in Imam-Reza Hospital from April 2009 to April 2011. Demographic data, paraclinical investigations and final diagnoses were analyzed with SPSS software.
Results: This study included 48 patients, 28 female and 20 male patients with a mean age of 50.7 years. The most common accompanying complaints were nausea and vomiting. Brain imaging was performed for all patients. The most frequent cause of headache was subarachnoid hemorrhage. Cerebral venous thrombosis and intracerebral hemorrhage were the next common causes.
Conclusion: Our findings revealed that the most frequent cause of thunderclap headache in our study population was subarachnoid hemorrhage. The second most frequent cause was cerebral venous thrombosis, and the least frequent causes were idiopathic intracranial hypertension and tension headache. A significant percentage of the causes of thunderclap headache can be life-threatening.
Conflict of Interest: Nil
Received: Apr27, 2012 Accepted: Dec11, 2012
http://sjku.muk.ac.ir/article-1-1072-en.pdf
Thunderclap headache
Subarachnoid hemorrhage
Cerebral venous thrombosis
per
Kurdistan University of Medical Sciences
Scientific Journal of Kurdistan University of Medical Sciences
1560-652X
2345-4040
2013-06
18
2
114
120
article
Frequency of sialic acid binding adhesin gene in Helicobacter pylori isolated from patient with gastroduodenal diseases
Zahra Pakbaz
1
Mohammad Hasan Shirazi
mhshirazi@tums.ac.ir
2
Mohammad Reza Pourman
3
Reza Ranjbar
4
Mostafa Hoseini
5
Ziba Vaise Malekshahi
6
Sara Haji Khani
7
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Baqiyatallah University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
ABSTRACT
Background and Aim: Sialic acid binding adhesin gene is one of the most important factors contributing to adhesin of Helicobacter pylori (H. pylori) to epithelial cell layer of stomach. The prevalence rates of sialic acid binding adhesin gene vary in different geographic areas. The aim of this study was to determine the frequency of sialic acid binding adhesin coding gene in the patients with different gastroduodenal diseases.
Materials and Methods: This is a cross-sectional study. One hundred twenty patients with GI symptoms were enrolled in this study. Two gastric biopsy specimens were taken from each of the patients for rapid urease test (RUT) and DNA extraction. Presence of H. pylori was investigated by RUT and urease A gene (ureA) PCR. sialic acid binding adhesin gene was detected by using gene specific primers.
Results: Among 120 samples, presence of H. pylori was confirmed in 82 cases, of which 64 strains (78%) were positive for sialic acid binding adhesin gene. The frequency of this gene was 84.6%, 86.7%, 77.8% and 72.2% for gartric cancer, gastric ulcer, duodenal ulcer and gastritis (%66.7) respectively.
Conclusion: The frequency of sialic acid binding adhesin gene in different samples was almost the same. Discrepancies in the frequency of this gene in different studies may be related to geographical diversity or use of different primers for detection of this gene.
Conflict of Interest: Nil
Received: Apr 6, 2012 Accepted: Sep 27, 2012
http://sjku.muk.ac.ir/article-1-1073-en.pdf
Helicobacter pylori
Sialic acid binding adhesin
Gastrointestinal disease.