Scientific Journal of Kurdistan University of Medical Sciences
مجله علمي دانشگاه علوم پزشكي كردستان
SJKU
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http://sjku.muk.ac.ir
54
journal54
1560-652X
2345-4040
sjku
10.22034
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jalali
1405
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2026
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ارزیابی مولکولی ارتباط پمپ افلاکس AdeABC با الگوی مقاومت آنتی بیوتیکی در جدایههای بالینی اسینتوباکتر بومانی
Molecular evaluation of the relationship between the AdeABC efflux pump with antibiotic resistance patterns in clinical isolates of Acinetobacter baumannii
میکروبیولوژی
Microbiology
پژوهشي اصیل
Original Research
<span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">زمینه و هدف</span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">:</span></span></b> <i><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">اسینتوباکتر بومانی</span></span></i> <span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">به دلیل دارا بودن انواعی از عوامل موثر در مقاومت آنتی­بیوتیکی مانند پمپهای افلاکس </span></span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""><span style="color:black">قادر به ایجاد انواع عفونتهای بیمارستانی </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">می باشد</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">این مطالعه با هدف بررسی فراوانی </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">ژنهای پمپ افلاکس </span></span><i><span dir="LTR" style="font-size:12.0pt"><span style="background:yellow">AdeABC</span></span></i><span style="font-size:12.0pt"><span b="" style="font-family:" zar=""> و سیستم تنظیمی آن </span></span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">در جدایه</span></span><span dir="LTR" style="font-size:12.0pt">­</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">های <i>اسینتوباکتر بومانی</i> و </span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" style="font-family:" zar=""><span style="color:black">ارزیابی ارتباط آن با مقاومت­های ضد میکروبی </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">انجام شد.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">مواد و روشها:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""><span style="color:black">الگوی مقاومت آنتی­ بیوتیکی</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> در 100 جدایه<i> اسینتوباکتر بومانی</i> </span></span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""><span style="color:black">با روش دیسک دیفیوژن</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""><span style="color:black">، مورد مطالعه قرار گرفت. سپس </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">حضور </span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:yellow"><span b="" style="font-family:" zar="">ژنهای </span></span></span><i><span dir="LTR" style="font-size:12.0pt"><span style="background:yellow">adeA</span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span style="background:yellow"><span b="" style="font-family:" zar="">، </span></span></span><i><span dir="LTR" style="font-size:12.0pt"><span style="background:yellow">adeB</span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span style="background:yellow"><span b="" style="font-family:" zar="">، </span></span></span><i><span dir="LTR" style="font-size:12.0pt"><span style="background:yellow">adeC</span></span></i><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="background:yellow"><span b="" style="font-family:" zar="">و ژن­های تنظیمی </span></span></span><i><span dir="LTR" style="font-size:12.0pt"><span style="background:yellow">adeR</span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span style="background:yellow"><span b="" style="font-family:" zar=""> و </span></span></span><i><span dir="LTR" style="font-size:12.0pt"><span style="background:yellow">adeS</span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> در جدایه ها با روش </span></span><span dir="LTR" style="font-size:12.0pt">PCR</span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""> مورد بررسی قرار گرفت. <span style="color:black">نتایج بدست آمده <span style="background:yellow">با نرم افزار </span></span></span></span> <span dir="LTR" style="font-size:12.0pt"><span style="background:yellow"><span style="color:black">SPSS</span></span></span><span style="font-size:12.0pt"><span style="background:yellow"><span b="" style="font-family:" zar=""><span style="color:black"> ورژن 16 و آزمون کای اسکوئر مورد تجزیه و تحلیل قرار</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""><span style="color:black"> گرفت.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" style="font-family:" zar="">یافته ها:</span></b> <span b="" style="font-family:" zar=""><span style="color:black">تحقیق حاضر نشان داد که در بین جدایه های <i>اسینتوباکتر بومانی، </i>86 جدایه <span style="background:yellow">دارای ژن </span></span></span><i><span dir="LTR" style="background:yellow">adeA</span></i><span lang="FA" style="background:yellow"><span b="" style="font-family:" zar=""><span style="color:black">، 80 جدایه دارای </span></span></span><span lang="FA" style="background:yellow"><span b="" style="font-family:" zar=""><span style="color:black">ژن </span></span></span><i><span dir="LTR" style="background:yellow">adeB</span></i><i> </i><span lang="FA" style="background:yellow"><span b="" style="font-family:" zar=""><span style="color:black">، 66 جدایه دارای ژن </span></span></span><i><span dir="LTR" style="background:yellow">adeC</span></i><span style="background:yellow"><span b="" style="font-family:" zar=""><span style="color:black"> ،</span></span></span><span lang="FA" style="background:yellow"><span b="" style="font-family:" zar=""><span style="color:black"> 81 جدایه دارای ژن </span></span></span><i><span dir="LTR" style="background:yellow">adeR</span></i><span lang="FA" style="background:yellow"><span b="" style="font-family:" zar=""><span style="color:black"> و 78 جدایه دارای ژن </span></span></span><i><span dir="LTR" style="background:yellow">adeS</span></i> <span lang="FA" style="background:yellow"><span b="" style="font-family:" zar=""><span style="color:black">بودند. میزان حضور ژن </span></span></span><i><span dir="LTR" style="background:yellow">adeA</span></i> <span lang="FA" style="background:yellow"><span b="" style="font-family:" zar=""><span style="color:black">و </span></span></span><i><span dir="LTR" style="background:yellow">adeR</span></i><span b="" style="font-family:" zar=""><span style="color:black"> در میان جدایه­ها بیش از دیگر ژن­ها بود.</span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" style="font-family:" zar="">نتیجه گیری:</span></b> <span b="" style="font-family:" zar=""><span style="color:black">نتایج نشان داد</span></span><span b="" style="font-family:" zar=""> که <span style="color:black">جدایههای <i>اسینتوباکتر بومانی</i> </span></span><span lang="FA" style="background:white"><span b="" style="font-family:" zar=""><span style="color:black">به </span></span></span><span b="" style="font-family:" zar="">اغلب آنتی بیوتیک ها بیش از 95 درصد مقاوم بودند و <span style="color:black">ژنهای </span></span><i><span dir="LTR" lang="EN-GB" style="background:yellow"><span style="color:black">adeA</span></span></i><span b="" style="font-family:" zar=""><span style="color:black">، </span></span><i><span dir="LTR" lang="EN-GB" style="background:yellow"><span style="color:black">adeB</span></span></i><span b="" style="font-family:" zar=""><span style="color:black">، </span></span><i><span dir="LTR" lang="EN-GB" style="background:yellow"><span style="color:black">adeR</span></span></i><span b="" style="font-family:" zar=""><span style="color:black"> و </span></span><i><span dir="LTR" lang="EN-GB" style="background:yellow"><span style="color:black">adeS</span></span></i> <span b="" style="font-family:" zar=""><span style="color:black">در بیش <span style="background:yellow">از 75</span></span></span><span lang="FA" style="background:yellow"><span b="" style="font-family:" zar=""><span style="color:black"> جدایه</span></span></span><span lang="FA" style="background:yellow"><span b="" style="font-family:" zar=""><span style="color:black"> وجود</span></span></span><span b="" style="font-family:" zar=""><span style="color:black"> داشت که </span></span><span b="" style="font-family:" zar="">با فنوتیپ </span><span b="" style="font-family:" zar="">مقاومت های بالای دارویی </span><span b="" style="font-family:" zar="">مشاهده شده در جدایه ها هماهنگ می باشد.</span><span style="font-family:"B Zar"" lang="FA"></span></span></span></span></span></span><br>
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<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background and Aim:</span></b> <i><span lang="EN" style="font-size:11.0pt">Acinetobacter baumannii</span></i><span lang="EN" style="font-size:11.0pt"> is capable of causing various nosocomial infections due to its presence of multiple factors that contribute to antibiotic resistance, such as efflux pumps.</span> <span lang="EN" style="font-size:11.0pt">This study aimed to investigate the frequency <span style="background:yellow">of <i>AdeABC</i> efflux</span> pump genes and its regulatory system in </span><i><span style="font-size:11.0pt">A. baumannii</span></i><span style="font-size:11.0pt"> isolates and to evaluate its relationship with antimicrobial resistance.</span><span style="font-size:11.0pt"></span></span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and Methods:</span></b> <span lang="EN" style="font-size:11.0pt">The antibiotic resistance pattern in 100 isolates of </span><i><span style="font-size:11.0pt">A. baumannii</span></i><span style="font-size:11.0pt"> was studied by disk diffusion method.</span> <span lang="EN" style="font-size:11.0pt">Then, the presence <span style="background:yellow">of <i>adeA</i>, <i>adeB</i>, <i>adeC</i> genes</span> and regulatory genes <i><span style="background:yellow">adeR</span></i> and <i><span style="background:yellow">adeS</span></i> in the isolates was investigated by PCR method.</span> <span lang="EN" style="font-size:11.0pt"><span style="background:yellow">The results were analyzed using chi-squared test in SPSS software</span></span><span style="font-size:11.0pt"><span style="background:yellow"> (version 16)</span></span><span lang="EN" style="font-size:11.0pt"><span style="background:yellow">. </span></span><span style="font-size:11.0pt"><span style="background:yellow"></span></span></span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="background:yellow">Results:</span></span></b> <span lang="EN" style="font-size:11.0pt"><span style="background:yellow">This study showed that among the isolates of </span></span><i><span style="font-size:11.0pt"><span style="background:yellow">A. baumannii</span></span></i><span lang="EN" style="font-size:11.0pt">, 86 isolates had the <i><span style="background:yellow">adeA</span></i> gene, 80 isolates <i><span style="background:yellow">adeB</span></i> gene, 66 isolates <i><span style="background:yellow">adeC</span></i> gene, 81 isolates <i><span style="background:yellow">adeR</span></i> gene, and 78 isolates <i><span style="background:yellow">adeS</span></i> gene.</span> <span lang="EN" style="font-size:11.0pt">The presence of <span style="background:yellow">the <i>adeA</i></span> and <i><span style="background:yellow">adeR</span></i> genes among the isolates was more than the other genes.</span><span style="font-size:11.0pt"></span></span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Conclusion: </span></b><span lang="EN" style="font-size:11.0pt">The results showed that </span><i><span style="font-size:11.0pt">A. baumannii</span></i><span lang="EN" style="font-size:11.0pt"> isolates were more <span style="background:yellow">than 95 percent resistant</span> to most antibiotics and <span style="background:yellow">the <i>adeA</i>, <i>adeB</i>,<i> adeR</i>, and <i>adeS</i></span> genes were present <span style="background:yellow">in more than 75 percent</span> of them, which is consistent with the high drug resistance phenotype observed in the isolates.</span><span style="font-size:11.0pt"></span></span></span><br>
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اسینتوباکتر بومانی، پمپ افلاکس، مقاومت آنتی بیوتیکی
Acinetobacter baumannii, Efflux pump, Antibiotic resistance
0
0
http://sjku.muk.ac.ir/browse.php?a_code=A-10-8358-2&slc_lang=fa&sid=1
Razieh
nazari
راضیه
نظری
Razieh.nazari@iau.ac.ir
5400319475328460073400
5400319475328460073400
Yes
Department of Microbiology, Qo.C, Islamic Azad University, Qom, Iran
گروه میکروبیولوژی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران
mahnaz
zarei
مهناز
زارعی
mahnaz.z2630@gmail.com
5400319475328460073401
0009-0004-4648-0625
No
Department of Microbiology, Qo.C, Islamic Azad University, Qom, Iran
گروه میکروبیولوژی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران