fa ارزیابی مولکولی ارتباط پمپ افلاکس AdeABC با الگوی مقاومت آنتی بیوتیکی در جدایه‌های بالینی اسینتوباکتر بومانی میکروبیولوژی Microbiology پژوهشي اصیل Original Research <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.1pt">زمینه</span></span></span></span></span></span></b><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.1pt">&nbsp;و هدف:</span></span></span></span></span></span></b>&nbsp;<i><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">اسینتوباکتر بومانی</span></span></i><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> به‌دلیل داشتن انواعی از عوامل مؤثر در مقاومت آنتی‌بیوتیکی، مانند پمپ‌های افلاکس، می‌تواند انواع عفونت‌های بیمارستانی را ایجاد کند. این مطالعه با هدف بررسی فراوانی ژن‌های پمپ‌ افلاکس </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">AdeABC</span></span></i><span style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> و سیستم تنظیمی آن در جدایه‌های اسینتوباکتر بومانی و ارزیابی ارتباط آن با مقاومت‌های ضدمیکروبی انجام شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#234cc3">مواد و روش‌ها:</span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">درباره الگوی مقاومت آنتی‌بیوتیکی در 100 جدایه اسینتوباکتر بومانی با روش دیسک دیفیوژن، مطالعه شد. سپس حضور ژن‌های </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeA</span></span></i><i><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">، </span></span></i><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeB</span></span></i><i><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">، </span></span></i><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeC</span></span></i><span style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> و ژن‌های تنظیمی </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeR</span></span></i><span style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeS</span></span></i><span style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> در جدایه‌ها با روش </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">PCR</span></span><span style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> بررسی شد. نتایج به‌دست‌آمده با نرم‌افزار </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">SPSS</span></span><span style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> نسخه 16 و آزمون کای اسکوئر تجزیه‌وتحلیل شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#234cc3">یافته‌ها:</span></span></span></b><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> تحقیق حاضر نشان داد که در بین جدایه‌های اسینتوباکتر بومانی، 86 جدایه ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeA</span></span></i><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">، 80 جدایه ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeB</span></span></i><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">، 66 جدایه ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeC</span></span></i><i><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">،</span></span></i><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> 81 جدایه ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeR</span></span></i><span style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> و 78 جدایه ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeS</span></span></i><span style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> داشتند. میزان حضور ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeA</span></span></i><span style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeR</span></span></i><span style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> در میان جدایه‌ها بیش از دیگر ژن‌ها بود.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#234cc3">نتیجه‌گیری:</span></span></span></b><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> نتایج نشان داد که جدایه‌های اسینتوباکتر بومانی به اغلب آنتی‌بیوتیک ها بیش از 95 درصد مقاوم بودند و ژن‌های </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeA</span></span></i><i><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">،</span></span></i> <i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeB</span></span></i><i><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">،</span></span></i> <i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeR</span></span></i><span style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">adeS</span></span></i><span style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> در بیش از 75 جدایه وجود داشت که با فنوتیپ مقاومت‌های بالای دارویی مشاهده‌شده در جدایه‌ها هماهنگ است.</span></span></span></span></span></span></span> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="font-family:Optima"><span style="color:#274cc3"><span style="letter-spacing:.15pt">Background and Aim:</span></span></span></span></b> <i><span style="font-size:9.0pt"><span style="font-family:Optima">Acinetobacter baumannii</span></span></i><span style="font-size:9.0pt"><span style="font-family:Optima"> is capable of causing various nosocomial infections due to the presence of multiple factors that contribute to antibiotic resistance, such as efflux pumps. This study aimed to investigate the frequency of <i>AdeABC</i> efflux pump genes and their regulatory system in A. baumannii isolates and evaluate their relationship with antimicrobial resistance</span></span><span dir="RTL" lang="FA" style="font-size:9.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span style="font-size:9.0pt"><span style="font-family:Optima"></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="font-family:Optima"><span style="color:#234cc3">Materials and Methods:</span></span></span></b><span style="font-size:9.0pt"><span style="font-family:Optima"> The antibiotic resistance pattern in 100 isolates of A. baumannii was studied by the disk diffusion method. Subsequently, the presence of <i>adeA</i>, <i>adeB</i>, and <i>adeC</i> genes and regulatory genes (<i>adeR</i> and <i>adeS</i>) in the isolates was investigated by the PCR method. The results were analyzed using the Chi-square test through SPSS software (version 16)</span></span><span dir="RTL" lang="FA" style="font-size:9.0pt"><span b="" nazanin="" style="font-family:">. </span></span><span style="font-size:9.0pt"><span style="font-family:Optima"></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="font-family:Optima"><span style="color:#234cc3">Results</span></span></span></b><span style="font-size:9.0pt"><span style="font-family:Optima">: This study showed that among the isolates of <i>A. baumannii</i>, 86 isolates had the <i>adeA</i> gene, 80 isolates had the <i>adeB</i> gene, 66 isolates had the <i>adeC</i> gene, 81 isolates had the <i>adeR</i> gene, and 78 isolates had the <i>adeS</i> gene. The presence of the<i> adeA</i> and <i>adeR</i> genes among the isolates was higher than that of the other genes</span></span><span dir="RTL" lang="FA" style="font-size:9.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span style="font-size:9.0pt"><span style="font-family:Optima"></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="font-family:Optima"><span style="color:#234cc3">Conclusion</span></span></span></b><span style="font-size:9.0pt"><span style="font-family:Optima">: The results showed that <i>A. baumannii </i>isolates were more than 95% resistant to most antibiotics, and the <i>adeA</i>, <i>adeB</i>, <i>adeR</i>, and <i>adeS</i> genes were present in more than 75% of them, which is consistent with the high drug resistance phenotype observed in the isolates</span></span><span dir="RTL" lang="FA" style="font-size:9.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span style="font-size:9.0pt"><span style="font-family:Optima"></span></span></span></span></span></span> اسینتوباکتر بومانی, پمپ افلاکس, مقاومت آنتی‌بیوتیکی Acinetobacter baumannii, Antibiotic resistance, Efflux pump 0 0 http://sjku.muk.ac.ir/browse.php?a_code=A-10-8358-2&slc_lang=fa&sid=1 Razieh nazari راضیه نظری Razieh.nazari@iau.ac.ir 5400319475328460074191 5400319475328460074191 Yes Department of Microbiology, Qo. C, Islamic Azad University, Qom, Iran گروه میکروبیولوژی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران mahnaz zarei مهناز زارعی mahnaz.z2630@gmail.com 5400319475328460074192 0009-0004-4648-0625 No Department of Microbiology, Qo. C, Islamic Azad University, Qom, Iran گروه میکروبیولوژی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران