Scientific Journal of Kurdistan University of Medical Sciences
مجله علمي دانشگاه علوم پزشكي كردستان
SJKU
General
http://sjku.muk.ac.ir
54
journal54
1560-652X
2345-4040
sjku
10.22102
en
jalali
1405
2
1
gregorian
2026
5
1
31
3
online
1
fulltext
fa
بررسی آزمایشگاهی اثرات سمیت سینرژیک Sophoraflavanone G استخراج شده از Sophora pachycarpa و داروی سیس پلاتین و دوستاکسل بر روی کارسینومای سلول سنگفرشی حفره دهان
In vitro investigation of synergistic effects of sophoraflavanon G extracted from Sophpra pachycarpa in combination with cisplatin/docetaxel on oral squamous cell carcinoma
بیوتکنولوژی
Biotechnology
پژوهشي اصیل
Original Research
<span style="font-size:12pt"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.1pt">زمینه</span></span></span></span></span></span></span></b><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.1pt"> و </span></span></span></span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.1pt">هدف</span></span></span></span></span></span></span></b><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.1pt">:</span></span></span></span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">کارسینوم سلول سنگ‏فرشی حفرۀ دهان شایعترین نئوپلاسم سرطانهای سر و گردن است که بیش از 90 درصد از تمام سرطانهای حفرۀ دهان را شامل میشود. نشان داده شده است که ترکیبات طبیعی اثرات ضدسرطانی قابل‏توجهی روی سلول‏های بدخیم دارند. از این میان، </span></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">Sophora pachycarpa</span></span></span></i> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">یکی از منابع ترکیبات</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">فلاونوئیدی مانند </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">sophoraflavanone G</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">(</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">SG</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">) است که دارای طیف وسیعی از خواص</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">بیولوژیکی ازجمله اثرات ضدسرطانی است. بنابراین، این مطالعه به‏منظور بررسی اثرات سیتوتوکسیک </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">SG</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">استخراج‏شده از </span></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">S</span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:" times="">. </span></span></span></i><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">pachycarpa</span></span></span></i> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">در ترکیب با داروی سیسپلاتین و یا دوستاکسل روی سلول‏های کارسینومای سلول سنگ‏فرشی حفرۀ دهان (سلول‏های </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">KB</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">) در شرایط برونتنی طراحی و انجام شد.</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times",serif"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:-.15pt">مواد و روشها</span></span></span></span></span></span></span></b><b><span lang="AR-SA" style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:-.15pt">:</span></span></span></span></span></span></b> <span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times=""><span style="letter-spacing:-.15pt">SG</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.15pt">از </span></span></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times=""><span style="letter-spacing:-.15pt">S. pachycarpa</span></span></span></span></i><span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.15pt"> با خلوص بیش از 95 درصد</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.15pt">استخراج شد. برای تعیین اثرات سیتوتوکسیک داروها از روش </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times=""><span style="letter-spacing:-.15pt">MTT</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.15pt">استفاده شد. برای بررسی اثرات</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.15pt">ترکیبی </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times=""><span style="letter-spacing:-.15pt">SG</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.15pt">و سیسپلاتین و یا دوستاکسل روی سلول‏های </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times=""><span style="letter-spacing:-.15pt">KB</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.15pt">، شاخص ترکیبی (</span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times=""><span style="letter-spacing:-.15pt">CI</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.15pt">) داروها ازطریق نرم‏افزار </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times=""><span style="letter-spacing:-.15pt">CompuSyn</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.15pt">مورد ارزیابی قرار گرفت</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:" times=""><span style="letter-spacing:-.15pt">.</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.15pt"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.1pt">یافتهها</span></span></span></span></span></span></span></b><b><span lang="AR-SA" style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.2pt">:</span></span></span></span></span></span></b><span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"> نتایج نشان داد </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">SG</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">اثرات سیتوتوکسیک قابل‏توجهی روی سلول‏های </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">KB</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">در مقایسه با </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">HEK-293</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">دارد. برخلاف سلول‏های نرمال، این ترکیب فعالیت ضدسرطانی سیسپلاتین/دوستاکسل را دربرابر سلول‏های </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">KB</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">افزایش داد. </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">SG</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">و سیسپلاتین/دوستاکسل در غلظتهای ترکیبی پایین، اثرات همافزایی قابل‏قبولی</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">بر سلول‏های </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">KB</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">با شاخص ترکیبی بین 33/0-43/0 ایجاد کردند. در کنار آن، ترکیب دارو و ماده تغییرات مورفولوژیکی قابل‏توجهی ایجاد کرد که احتمالا میتوان آن را به‏عنوان</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">شاخص آپوپتوز در نظر گرفت.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:-.15pt">نتیجهگیری:</span></span></span></span></span></span></span></b><span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:"> درمجموع، </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span style="font-family:" times="">SG</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:90%"><span b="" nazanin="" style="font-family:">میتواند کاندید مناسبی برای درمان هدفمند سرطان دهان معرفی شود. </span></span></span></span></span></span></span></span></span>
<span style="font-size:12pt"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="font-family:Optima"><span style="color:#274cc3"><span style="letter-spacing:.15pt">Background and Aim:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:Optima">Oral squamous cell carcinoma is the most common neoplasm in the oral cavity, accounting for 90% of all oral cavity cancers. It has been shown that natural compounds could exert significant anti-cancer effects on malignant cells. Amongst Sophora species, Sophora pachycarpa, a source of flavonoid compounds such as sophoraflavanone G (SG), possesses a wide range of biological properties,</span></span><span style="font-size:9.0pt"><span style="font-family:Optima"> including anticancer effects. Therefore, this study was conducted to investigate the cytotoxic effects of SG extracted from <i>S. pachycarpa,</i> in combination with cisplatin or docetaxel, on </span></span><span style="font-size:9.0pt"><span style="font-family:Optima">oral squamous cell carcinoma</span></span><span style="font-size:9.0pt"><span style="font-family:Optima"> cells. </span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="font-family:Optima"><span style="color:#274cc3">Materials and Methods: </span></span></span></b><span style="font-size:9.0pt"><span style="font-family:Optima">SG was extracted from <i>S. pachycarpa</i> with more than 95% purity. The MTT assay was used to determine the cytotoxic effects of the drugs. To investigate the combinatorial effects of SG and drugs on KB cells, the combination index (CI) was evaluated using CompuSyn software.</span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="font-family:Optima"><span style="color:#274cc3"><span style="letter-spacing:.15pt">Results:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:Optima">SG exhibited considerable cytotoxic effects on KB cells compared with HEK-293 cells. In normal cells, this compound enhanced the anticancer activity of cisplatin/docetaxel against KB cells. The combination index showed that SG and cisplatin/docetaxel at low combination concentrations produced synergistic cytotoxic effects on KB cells, with values between 0.3 and 0.4. Alongside, SG+cisPt/DTX caused noticeable morphological changes that could be considered an apoptotic indicator. </span></span><span lang="X-NONE" style="font-size:9.0pt"><span style="font-family:Optima"></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="font-family:Optima"><span style="color:#274cc3"><span style="letter-spacing:-.1pt">Conclusion:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:Optima">These results suggest the potential of SG to enhance the anticancer activity of cisplatin/docetaxel against KB cell lines. It also reduces the cytotoxic effects of cisPt/DTX on HEK-29 cells. Therefore, SG could be a promising candidate for the </span></span><span style="font-size:9.0pt"><span style="font-family:Optima">targeted treatment of oral cancer</span></span><span dir="RTL" lang="FA" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span style="font-size:9.0pt"><span style="font-family:Optima"></span></span></span></span></span></span>
Sophora pachycarpa, Sophoraflavanone G, سیسپلاتین, دوستاکسل, کارسینوم سلول سنگفرشی حفرۀ دهان, ردۀ سلولی KB, سمیت سلولی, اثرات ترکیبی
Cell Cytotoxicity, Cisplatin, Combinational Effects, Docetaxel, KB Cell Line, Sophoraflavanone G, Sophora pachycarpa, Squamous Cell Carcinoma
0
0
http://sjku.muk.ac.ir/browse.php?a_code=A-10-7902-1&slc_lang=fa&sid=1
Manouchehr
Teymouri
منوچهر
تیموری
manochehr.teimory@gmail.com
5400319475328460074150
5400319475328460074150
No
. Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
مرکز تحقیقات فرآوردههای طبیعی و گیاهان دارویی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران
Negar
Abdolahzadeh
نگار
عبدالله زاده
abdolahzadeh.negar@gmail.com
5400319475328460074151
5400319475328460074151
No
Department of Advanced Technologies, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
گروه فناوریهای نوین، دانشکده پزشکی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران
Saied
Mahmoodi
سعید
محمودی
saidmahmoodi2020@gmail.com
5400319475328460074152
0009-0001-3479-6713
No
School of Dentistry, North Khorasan University of Medical Sciences, Bojnurd, Iran
دانشکده دندانپزشکی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران
Fatemeh
Didari Meymand
فاطمه
دیداری میمند
fatemehdidari2015@gmail.com
5400319475328460074153
0009-0003-4285-0070
No
School of Dentistry, North Khorasan University of Medical Sciences, Bojnurd, Iran
دانشکده دندانپزشکی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران
Milad
Iranshahi
میلاد
ایرانشاهی
Iranshahym@mums.ac.ir
5400319475328460074154
5400319475328460074154
No
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
گروه فارماکوگنوزی، دانشکده داروسازی، دانشگاه علوم پزشکی مشهد، مشهد، ایران
Javid
Rasekhi
جاوید
راسخی
J.Rasekhi@nkums.ac.ir
5400319475328460074155
5400319475328460074155
No
School of Dentistry, North Khorasan University of Medical Sciences, Bojnurd, Iran
دانشکده دندانپزشکی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران
Mahla
Abdari
مهلا
آبداری
mahla_abdari@yahoo.com
5400319475328460074156
0009-0003-2837-0716
No
Comprehensive Research Laboratory, North Khorasan University of Medical Sciences, Bojnurd, Iran
آزمایشگاه جامع تحقیقات، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران
Alireza
Afshari-Safavi
علیرضا
افشاری صفوی
alireza.afsharisafavi@gmail.com
5400319475328460074157
5400319475328460074157
No
Department of Biostatistics and Epidemiology, School of Health, North Khorasan University of Medical Sciences, Bojnurd, Iran
گروه آمار زیستی و اپیدمیولوژی، دانشکده بهداشت، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران
Javad
Hashemi
جواد
هاشمی
J.hashemi@nkums.ac.ir
5400319475328460074158
5400319475328460074158
No
Department of Clinical Biochemistry, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران
Fahimeh
Khaghani
فهیمه
خاقانی
F.khaghani@nkums.ac.ir
5400319475328460074159
0009-0000-6128-8691
No
School of Dentistry, North Khorasan University of Medical Sciences, Bojnurd, Iran
دانشکده دندانپزشکی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران
Samaneh
Mollazadeh
سمانه
ملازاده
samanehmollazadeh@yahoo.com
5400319475328460074160
5400319475328460074160
Yes
Department of Medical Biotechnology, School of Medicine, North Khorasan University of Sciences, Bojnurd, Iran
گروه بیوتکنولوژی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران