Scientific Journal of Kurdistan University of Medical Sciences

fa بررسی اثر فاکتورهای بیماریزای هلیکوباکترپیلوری (vacA، cagA، oipA و iceA) بر روند بیماریزایی این باکتری در بیماران مبتلا به سوء هاضمه Effects of H. pylori Virulence Factors (vacA, cagA, oipA and iceA) on Its Pathogenicity in Patients Suffering from Gastroduodenal Disorders میکروبیولوژی Microbiology پژوهشي اصیل Original Research زمینه و هدف: هلیکوباکتر پیلوری، باکتری مسبب مشکلات گوارشی و سوء هاضمه می باشد که در نهایت می تواند منجر به سرطان معده شود. ژنهای مربوط به فاکتورهای بیماریزای هلیکوباکتر پیلوری عبارتند از : vacA، cagA، oipA و .iceA <a name="_Hlk82680777">این مطالعه در  غرب ایران و در بیماران مبتلا به سوء هاضمه، با در نظر گرفتن فراوانی فاکتورهای بیماری زای هلیکوباکترپیلوری، به ارتباط این فاکتورها با یافته های پاتولوژیک مربوطه تمرکز کرده است.</a> مواد و روش ها: در این مطالعه از 120 بیماران مبتلا به سوء هاضمه (60 بیمار دارای التهاب معده و 60 بیمار دارای پپتیک اولسر) نمونه های بیوپسی معده به عمل آمد. ژنوتایپ فاکتورهای بیماری زای هلیکوباکترپیلوری با استفاده از روش واکنش زنجیره ای پلیمراز  انجام گردید. تجزیه و تحلیل داده ها با استفاده از نرم افزار SPSS 16.0  انجام شد. مقادیر  0.05> p از نظر آماری معنی دار در نظر گرفته شد.  یافته‌ها: فراوانی ژنوتایپ های بررسی شده در این مطالعه به ترتیب عبارتند از: vacA (%51/7s1  ، %21/7s2 ، 15/8%s1s2 ، 32/51%m1 ، %45m2 ،7/5% m1m2 ، %29/2s1m1 ، % 27/5s1m2 ،7/5%s2m1 ،7/5 %  s2m2)، 64/2 % cagA، 57/5%oipA ، 55 % iceA2 و 15 % iceA1/iceA2. همچنین بین حضور فاکتورهای بیماری زایcagA  (0/013 =p)، vacA (p=0/041) و  iceA (p=0/035) و نوع بیماری (گاستریت و پپتیک اولسر) از سوی دیگر ارتباط معنی داری مشاهده گردید. ارتباط معنی دار بین فاکتورهای بیماریزا و یافته های پاتولوژیک عبارتند از: فاکتور iceA با التهاب حاد (039/0 p=) و همچنین فاکتورهای oipA(p=0/00) و vacA (p=0/011) با میزان تراکم باکتریایی هلیکوباکترپیلوری به ترتیب (p=0/00)  و(p=0/011)را دارند. Background and Aim: H. pylori can cause digestive problems and eventually can lead to gastric cancer. This study deals with the relationship between H. pylori virulence factors and pathological findings in the patients with gastroduodenal disorders in a particular geographical area in the west of Iran. Materials and Methods: In this study, gastric biopsies were taken from 120 patients with indigestion (60 patients with gastritis and 60 patients with peptic ulcer). H. pylori virulence factors, vacA, cagA, oipA and iceA were determined by polymerase chain reaction (PCR). SPSS 16.0 software was used for data analysis. p≤0.05 was considered statistically significant. Results: The frequencies of the genotypes were as following: vacA (s1 51.7 %, s2 21.7%, s1s2 15.8 %, m1 32.51 %, m2 45 %, m1m2 7.5 %, s1m1 29.2 %, s1m2 27.5%, s2m1 7.5 %, s2m2 7.5 %), cagA 64.2%, oipA 57.5 %, iceA2 55% and iceA1/iceA2 15% respectively. Also, a significant relationship was observed between virulence factors such as cagA (p=0.013), vacA (p=0.041) and iceA (p=0.035) on one hand, and type of disorder (gastritis and peptic ulcer) on the other hand. Considering the relationships between the virulence factors and pathological findings, a significant relationship was found between iceA and acute inflammation (p=0.039). Also oipA and vacA showed significant relationships with bacterial density of H. pylori (p=0,000) and (p=0.011) respectively. Conclusion: The results of this study showed role of vacA and cagA in the type of disease and the impact of oipA and iceA in microscopic findings. Background and Aim: H. pylori can cause digestive problems and eventually can lead to gastric cancer. This study deals with the relationship between H. pylori virulence factors and pathological findings in the patients with gastroduodenal disorders in a particular geographical area in the west of Iran. Materials and Methods: In this study, gastric biopsies were taken from 120 patients with indigestion (60 patients with gastritis and 60 patients with peptic ulcer). H. pylori virulence factors, vacA, cagA, oipA and iceA were determined by polymerase chain reaction (PCR). SPSS 16.0 software was used for data analysis. p≤0.05 was considered statistically significant. Results: The frequencies of the genotypes were as following: vacA (s1 51.7 %, s2 21.7%, s1s2 15.8 %, m1 32.51 %, m2 45 %, m1m2 7.5 %, s1m1 29.2 %, s1m2 27.5%, s2m1 7.5 %, s2m2 7.5 %), cagA 64.2%, oipA 57.5 %, iceA2 55% and iceA1/iceA2 15% respectively. Also, a significant relationship was observed between virulence factors such as cagA (p=0.013), vacA (p=0.041) and iceA (p=0.035) on one hand, and type of disorder (gastritis and peptic ulcer) on the other hand. Considering the relationships between the virulence factors and pathological findings, a significant relationship was found between iceA and acute inflammation (p=0.039). Also oipA and vacA showed significant relationships with bacterial density of H. pylori (p=0,000) and (p=0.011) respectively. Conclusion: The results of this study showed role of vacA and cagA in the type of disease and the impact of oipA and iceA in microscopic findings. Background and Aim: H. pylori can cause digestive problems and eventually can lead to gastric cancer. This study deals with the relationship between H. pylori virulence factors and pathological findings in the patients with gastroduodenal disorders in a particular geographical area in the west of Iran. Materials and Methods: In this study, gastric biopsies were taken from 120 patients with indigestion (60 patients with gastritis and 60 patients with peptic ulcer). H. pylori virulence factors, vacA, cagA, oipA and iceA were determined by polymerase chain reaction (PCR). SPSS 16.0 software was used for data analysis. p≤0.05 was considered statistically significant. Results: The frequencies of the genotypes were as following: vacA (s1 51.7 %, s2 21.7%, s1s2 15.8 %, m1 32.51 %, m2 45 %, m1m2 7.5 %, s1m1 29.2 %, s1m2 27.5%, s2m1 7.5 %, s2m2 7.5 %), cagA 64.2%, oipA 57.5 %, iceA2 55% and iceA1/iceA2 15% respectively. Also, a significant relationship was observed between virulence factors such as cagA (p=0.013), vacA (p=0.041) and iceA (p=0.035) on one hand, and type of disorder (gastritis and peptic ulcer) on the other hand. Considering the relationships between the virulence factors and pathological findings, a significant relationship was found between iceA and acute inflammation (p=0.039). Also oipA and vacA showed significant relationships with bacterial density of H. pylori (p=0,000) and (p=0.011) respectively. Conclusion: The results of this study showed role of vacA and cagA in the type of disease and the impact of oipA and iceA in microscopic findings. Background and Aim: H. pylori can cause digestive problems and eventually can lead to gastric cancer. This study deals with the relationship between H. pylori virulence factors and pathological findings in the patients with gastroduodenal disorders in a particular geographical area in the west of Iran. Materials and Methods: In this study, gastric biopsies were taken from 120 patients with indigestion (60 patients with gastritis and 60 patients with peptic ulcer). H. pylori virulence factors, vacA, cagA, oipA and iceA were determined by polymerase chain reaction (PCR). SPSS 16.0 software was used for data analysis. p≤0.05 was considered statistically significant. Results: The frequencies of the genotypes were as following: vacA (s1 51.7 %, s2 21.7%, s1s2 15.8 %, m1 32.51 %, m2 45 %, m1m2 7.5 %, s1m1 29.2 %, s1m2 27.5%, s2m1 7.5 %, s2m2 7.5 %), cagA 64.2%, oipA 57.5 %, iceA2 55% and iceA1/iceA2 15% respectively. Also, a significant relationship was observed between virulence factors such as cagA (p=0.013), vacA (p=0.041) and iceA (p=0.035) on one hand, and type of disorder (gastritis and peptic ulcer) on the other hand. Considering the relationships between the virulence factors and pathological findings, a significant relationship was found between iceA and acute inflammation (p=0.039). Also oipA and vacA showed significant relationships with bacterial density of H. pylori (p=0,000) and (p=0.011) respectively. Conclusion: The results of this study showed role of vacA and cagA in the type of disease and the impact of oipA and iceA in microscopic findings. Background and Aim: H. pylori can cause digestive problems and eventually can lead to gastric cancer. This study deals with the relationship between H. pylori virulence factors and pathological findings in the patients with gastroduodenal disorders in a particular geographical area in the west of Iran. Materials and Methods: In this study, gastric biopsies were taken from 120 patients with indigestion (60 patients with gastritis and 60 patients with peptic ulcer). H. pylori virulence factors, vacA, cagA, oipA and iceA were determined by polymerase chain reaction (PCR). SPSS 16.0 software was used for data analysis. p≤0.05 was considered statistically significant. Results: The frequencies of the genotypes were as following: vacA (s1 51.7 %, s2 21.7%, s1s2 15.8 %, m1 32.51 %, m2 45 %, m1m2 7.5 %, s1m1 29.2 %, s1m2 27.5%, s2m1 7.5 %, s2m2 7.5 %), cagA 64.2%, oipA 57.5 %, iceA2 55% and iceA1/iceA2 15% respectively. Also, a significant relationship was observed between virulence factors such as cagA (p=0.013), vacA (p=0.041) and iceA (p=0.035) on one hand, and type of disorder (gastritis and peptic ulcer) on the other hand. Considering the relationships between the virulence factors and pathological findings, a significant relationship was found between iceA and acute inflammation (p=0.039). Also oipA and vacA showed significant relationships with bacterial density of H. pylori (p=0,000) and (p=0.011) respectively. Conclusion: The results of this study showed role of vacA and cagA in the type of disease and the impact of oipA and iceA in microscopic findings. هلیکوباکترپیلوری, گاستریت, VacA,CagA , IceA H. pylori, Gastritis, VacA, CagA, IceA 74 86 http://sjku.muk.ac.ir/browse.php?a_code=A-10-6363-1&slc_lang=fa&sid=1 Amin Talebi Abadi فرید زندی zandifarid@gmail.com 5400319475328460050458 5400319475328460050458 No Ph.D. Candidate, Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. دانشجوی دکتری، گروه باکتری شناسی پزشکی، دانشکده پزشکی، دانشگاه تربیت مدرس، تهران، ایران. ک امین طالبی بزمین آبادی amin.talebi@modares.ac.ir 5400319475328460050459 5400319475328460050459 Yes Assistant Professor, Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran., دانشیار، گروه باکتری شناسی پزشکی، دانشکده پزشکی، دانشگاه تربیت مدرس، تهران، ایران.، اشرف محبتی مبارز mmmobarez@modares.ac.ir 5400319475328460050460 5400319475328460050460 No Professor, Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran استاد، گروه باکتری شناسی پزشکی، دانشکده پزشکی، دانشگاه تربیت مدرس، تهران، ایران. محمد عبدی abdi@muk.ac.ir 5400319475328460050461 5400319475328460050461 No Assistant Professor, Department of Clinical biochemistry, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj. Iran دانشیار، گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی کردستان، سنندج، ایران.