TY - JOUR JF - HBI_Journals JO - SJKU VL - 22 IS - 4 PY - 2017 Y1 - 2017/9/01 TI - Protective effects of hydro-alcoholic garlic extract on spermatogenic disorders in streptozotocin-induced diabetic C57BL/6 mice TT - بررسی اثرات محافظتی عصاره هیدروالکلی سیر بر اختلالات اسپرماتوژنز در موش های C57BL/6 دیابتی شده با استرپتوزوتوسین N2 - Background and Aim: Diabetes can cause sexual dysfunction including spermatogenic disorders in the diabetic patients. The aim of the present study was to investigate the antioxidant effects of garlic extract in diabetic mice. Materials and Methods: Diabetes was induced by multiple low-dose streptozotocin injections (40 mg/kg/day for 5 consecutive days), in male C57BL/6 mice (15-20 gr body weight). After induction of diabetes, the mice were divided into 4 groups: group 1 (normal control group); group 2 (diabetic control group); group 3 (treatment with garlic extract 200 mg/kg for 35 days) and group 4 (treatment with garlic extract 400 mg/kg for 35 days). Mice were euthanized on the 35th day and serum testosterone levels were estimated. Testes and epididymis were removed for sperm evaluation and measurement of nitric oxide (NO) levels and total antioxidant capacity (TAC). Results: Garlic treatment significantly improved semen parameters and increased serum level of testosterone (p<0.05). Aside from reducing the elevated tissue nitric oxide level, garlic increased total antioxidant capacity in testis tissue samples. Conclusion: This study showed potent antioxidant effect of hydro-alcoholic garlic extract on the testes of mice against type 1 diabetes -induced oxidative stress. Keywords: Type 1 diabetes, Garlic, Spermatogenesis, Nitric oxide, Total antioxidant capacity. Received: Nov 21, 2016 Accepted: May 13, 2017 SP - 8 EP - 17 AU - Soleimanzadeh, Ali AU - Malekifard, Farin AU - Kabirian, Alireza AD - Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran KW - Type 1 diabetes KW - Garlic KW - Spermatogenesis KW - Nitric oxide KW - Total antioxidant capacity. UR - http://sjku.muk.ac.ir/article-1-3390-en.html DO - 10.22102/22.4.8 ER -