TY - JOUR JF - HBI_Journals JO - SJKU VL - 22 IS - 1 PY - 2017 Y1 - 2017/4/01 TI - The effect of allopurinol on high glucose- induced neurotoxicity in PC12 cells TT - بررسی تاثیر آلوپورینول بر نوروتوکسیسیتی ناشی از غلظت بالای گلوکز در سلول های PC12 N2 - Background and Aim: Hyperglycemia which occurs in diabetes is one of the main factors which can lead to serious complications such as diabetic neuropathy. There is evidence that allopurinol has neuroprotective effect against many types of damaging stimuli. The present study investigated the effects of allopurinol on high glucose induced neurotoxicity in PC12 cells as a suitable in-vitro model for evaluation of neuronal functions. Material and Methods: Neurotoxicity was induced by high glucose concentration, and cells were exposed to allopurinol in the presence or absence of high glucose concentration. Cell viability was assessed by MTT assay. Biochemical markers of oxidative stress were investigated by measurement of lipid peroxidation (LPO), total thiol groups, and total antioxidant power (TAP). Results: The present results indicated that allopurinol significantly inhibited high glucose- induced cell death in PC12 cells. Furthermore, treatment with allopurinol decreased lipid peroxidation level. It also increased the total thiol groups and TAP. Conclusion: These findings showed protective effects of allopurinol on HG-induced cell death in PC12 cells, which may be related to its antioxidant effect and inhibition of oxidative stress. Keywords: PC12 cells, Glucose, Neurotoxicity, Allopurinol. Received: Apr 23, 2016 Accepted: Nov 26, 2016 SP - 1 EP - 10 AU - Aminzadeh, Azadeh AD - Department of Pharmacology and Toxicology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran KW - PC12 cells KW - Glucose KW - Neurotoxicity KW - Allopurinol. UR - http://sjku.muk.ac.ir/article-1-2955-en.html DO - 10.22102/22.1.1 ER -