TY - JOUR JF - HBI_Journals JO - SJKU VL - 18 IS - 3 PY - 2013 Y1 - 2013/10/01 TI - Comparison of adenosine deaminase level in HIV positive patients co-infected with hepatitis B and C and healthy subjects TT - مقایسه سطح سرمی آنزیم Adenosine Deaminase (ADA) در بیماران HIV مثبت مبتلا به هپاتیت B و C با افراد سالم و غیر مبتلا به هپاتیت N2 - ABSTRACT Background and Aim: Measurement of adenosine deaminase and its isoenzymes in serum has been used for diagnosis and differentiation of intracellular infections and malignancies of immune cells. The aim of this study was to compare ADA levels and its isoenzymes in HIV positive patients and HIV positive patients co-infected with hepatitis B and C and healthy individuals, as a quick and low-cost diagnostic biochemical marker. Material and Methods: This historical cohort study included 97 HIV positive IDU patients. After obtaining blood samples, serum was separated and examined for hepatitis B and C. We used Guisti method for measurment of ADA activity and EHNA inhibitor for the activity of its isoenzymes. Data analysis was performed by SPSS 16 (SPSS Inc., Chicago). Results: Our results showed that there was a high prevalence of HBV and HCV in HIV positive IV drug users. We found that total ADA activity in the patients groups was significantly higher than control group. Also HIV-HCV group showed a higher activity of this enzyme in comparison to HIV positive groups. Significant differences were found in regard to ADA2 isoenzymes among the four groups (P SP - 93 EP - 103 AU - Khodadadi, Iraj AU - Abdi, Mohammad AU - Javid, Saman AU - Ahmadi, Abas AU - Roshani, Daem AU - Rahbari, Rezgar AU - Sharifipour, Mozhdeh AU - Shahmohammad-nezhade, Shiva AU - Khatooni, Zahed AD - Kurdistan University of Medical Sciences KW - IV drug user KW - Adenosine deaminase KW - Isoenzyme KW - HIV positive KW - HBV-HBV positive KW - HIV-HCV positive KW - CD4+. UR - http://sjku.muk.ac.ir/article-1-1131-en.html ER -