TY - JOUR T1 - Comparative study of the effect of garlic tablet and blueberry extract on Cryptosporidum parvum oocysts in HANK solution TT - بررسی مقایسه ای تاثیر قرص سیر و عصاره الکلی ذغال اخته بر روی اووسیست کریپتوسپوریدیوم پارووم در محیط HANK JF - HBI_Journals JO - HBI_Journals VL - 17 IS - 2 UR - http://sjku.muk.ac.ir/article-1-800-en.html Y1 - 2012 SP - 53 EP - 60 KW - Blue berry cherry KW - Garlic KW - Cryptosporidium parvum KW - Parasite. N2 - Background and Aim: Cryptosporidium is a common cause of diarrhea worldwide in both humans and animals. It causes severe and chronic diseases in HIV positive people and neonates. As yet no effective treatment has been found for it. The aim of this study was to evaluate the therapeutic effects of blue berry cherry, garlic and mixture of them on the Cryptosporidium parvum oocysts in HANK medium. Material and methods: Different concentrations of blue berry cherry extract, garlic tablets and mixture of them, with dilutions of %100, %50, %25 and %12.5, were prepared and sterilized by filter 0.22. The oocysts were separated from the stool of young calves suffering from diarrhea and were concentrated by use of sucrose and Shyter methods. 100λ of the concentrate containing 2×106 oocysts and 900λ of the above mentioned dilutions (blue berry cherry extract, garlic tablets and mixture of them) were poured into the Ependrof vials (pipes) and kept into an incubator at 37ºC for 24 to 48 hours (tests were performed in triplicates). Results: The results showed that all concentrations were effective in reducing Cryptosporidium parvum oocysts, but 1/100 garlic concentration, was more effective than blue berry cherry at the same concentration (P M3 ER -