TY - JOUR T1 - The effect of guidance channel on sciatic nerve repair in adult rats TT - اثر کانال راهنمای عصب در ترمیم عصب سیاتیک در موش صحرایی بالغ JF - HBI_Journals JO - HBI_Journals VL - 16 IS - 3 UR - http://sjku.muk.ac.ir/article-1-581-en.html Y1 - 2011 SP - 9 EP - 19 KW - Guidance channel KW - NGF KW - PVDF KW - Sciatic nerve repair KW - Spinal motor neurons KW - Tracing. N2 - ABSTRACT Background and Aim: Peripheral nerve lesions are a challenge for neurosurgeons and different surgical repairing methods are applied for the treatment of this problem. This study was conducted to evaluate the poled polyvinelidene fluoride (PVDF) tube filled with nerve growth factor (NGF) and collagen gel as a substitute for nerve autograft. Material and Methods: In this experimental study the left sciatic nerve was manipulated in 50 male Wistar rats and then the animals were divided randomly into five groups. In the epineural group the injured nerves were repaired by end to end suture. In the rats with autograft a 10 mm piece of sciatic nerve was rotated through 180° and sutured in the nerve gap. In the nerve guidance channel group (NGC), polarized piezoelectric PVDF tube containing NGF and collagen gel was replaced in the gap and in the axotomy group two nerve ends were hidden among muscles. The left sciatic nerve was exposed but not transected in the sham group. After two months L4-L6 segment neurons of spinal cord were studied histologically and by immunohistochemical and axonal DiI tracing. The collected data were analyzed by one way ANOVA, LSD and paired t- test. Results: The mean number of Bax positive cells and labeled motor neurons increased significantly in axotomy and sham group respectively, compared to the other groups (P M3 ER -