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Determination of malachite green and leucomalachite green residues in rainbow trout in Sanandaj City
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Reza Rezaee1    , Afshin Maleki2 , MAHDI SAFARI2 , BEHZAD SHAHMORADI2 , ALI JAFARI3 , OMID GIAHI2 , SHIVA ZANDI2 , NIKU KHALEDIAN4 , SHIMA SHARIFI4 , Shadieh Mohammadi2 |
1- Environmental Health Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran , rezaee.eng@gmail.com
2- Environmental Health Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
3- Department of environmental health engineering, faculty of health and nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran
4- Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran |
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Abstract: (4360 Views) |
Background and Aim: Malachite green (MG) is an organic compound which his used as a dye in textile industry. This compound is applied incorrectly and illegally in aquiculture as an antimicrobial agent. This compound has mutagenic and carcinogenic effects.
Materials and Methods: In this study we determined the concentrations of MG and leucomalachite green (LMG) in the muscle tissue of 90 rainbow trouts (Oncorhynchus mykiss) collected from the local markets in Sanandaj City within a period of six months in 2018. In order to measure the residues of MG and LMG, we used enzyme-linked immunosorbent assay (ELISA) based on the European Union Decision 2002/657/EC.
Results: The results showed presence of MG and LMG in all 90 samples: The residues of MG in the samples were as follows: 1-2 μg/Kg in 41 (6.45%), more than 2 μg/Kg in 45 (50%) and less than 1 μg/Kg in 4 samples (4.4%). Furthermore, the residues of LMG in the samples were as follows: more than 2 μg/Kg in 13 (45.14%), 1-2 μg/Kg in 67 (44.74%) and less than 1 μg/Kg in 10 samples (11.11%). Moreover, the limit of detection (LOD) in the method was 0.43 μg/Kg.
Conclusions: It is concluded that MG is illegally applied in fish farms, which can be a threat to human health and the environment. |
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| Keywords: Malachite green, Leucomalachite green, Rainbow trout, ELISA, Sanandaj |
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Full-Text [PDF 555 kb]
(3008 Downloads)
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Type of Study: Original Research |
Subject:
Health and Food quality control
Received: 2020/01/18 | Accepted: 2020/03/10 | Published: 2020/07/22
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