Background and Aim: Recent studies on the treatment of melanoma, have focused on designing efficient methods with low side effects. The aim of the present study was to investigate the effect of quercetin- omega-3 and chrysin- omega-3 complexes on proliferation and apoptosis in the A-375 melanoma cell line.
Materials and Methods: The cells were cultured and then were treated with different concentrations of quercetin- omega-3 and chrysin- omega-3 complexes. MTT assay and flow cytometry were used to evaluate the effects of the above compounds on the rate of cell proliferation and apoptosis.
Results: Chrysin- omega-3 and quercetin- omega-3 complexes in the experimental concentrations, inhibited cell growth in a time and concentration-dependent manner. No significant changes in cell growth rate were observed after 24 and 48 hours of treatment with 0-125μM of chrysin- omega-3 complex. However, concentrations of 150, 175, and 200μM chrysin- omega-3 complex significantly decreased the growth of melanoma cells after 24 and 48h (p <0.05). After 72hours, the inhibitory effect was more potent, so that the inhibitory effect was also seen in lower concentrations (100 and 125μm). The highest rate of induction of apoptosis was observed after 72hours (p< 0.05). In regard to quercetin- omega-3 complex, the results of the study were similar.
Conclusion: Antiproliferative effects of quercetin- omega-3 and chrysin- omega-3 complexes on melanoma cells are very promising, and suggest these compounds can be considered as potential therapeutic candidates.