:: Volume 24, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2019) ::
SJKU 2019, 24(3): 34-42 Back to browse issues page
Effect of chromatin condensation on frozen-thawed sperm DNA integrity in normozoospermic men
Farhad Golshan-Iranpour1 , Fahimeh Zamani Rarani2 , Gholam Reza Dashti 3
1- Saint Maryam Fertility and Infertility Center, Shahid Beheshti Hospital, Isfahan, Iran.
2- Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
3- Saint Maryam Fertility and Infertility Center, Shahid Beheshti Hospital, Isfahan, Iran. , dashti@med.mui.ac.ir
Abstract:   (297 Views)
Background and Aim: Sperm cryopreservation is a common technique used for management of male infertility. But this method has detrimental effects on sperm DNA and chromatin quality. Evaluation of different markers associated with the health of genetic material of sperm is beneficial for determination of the fertility of sperm. The aim of this study was to assess the effect of sperm chromatin condensation on frozen-thawed sperm DNA integrity in normozoospermic men.
Material and Methods: In this experimental study, 30 semen samples from normozospermia men were cryopreserved for two weeks with a common technique used in most infertility centers, at -196 ° C and then thawed. Samples before and after freezing were evaluated for abnormal chromatin condensation, DNA denaturation and DNA fragmentation by toluidine blue (TB) staining, acridine orange (AO) staining and sperm chromatin dispersion (SCD) test respectively.
Results: Before freezing, we found a significant correlation only between abnormal chromatin condensation (evaluated by TB) and DNA denaturation (assessed by AO) (p < 0.05). While after cryopreservation correlation was found between abnormal chromatin condensation and DNA denaturation and fragmentation (p < 0.05).
Conclusion: Abnormal chromatin condensation can make DNA susceptible to denaturation and fragmentation.
Keywords: sperm, DNA, chromatin, normozoospermic
Full-Text [PDF 273 kb]   (105 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/12/8 | Accepted: 2019/07/7


XML   Persian Abstract   Print



Volume 24, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2019) Back to browse issues page