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Effect of prenatal immobilization stress on spatial memory, anxiety-like behavior and brain BDNF concentration in the F1 generation male mice
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Nastaran Aliabadi1   , Hedayat Sahraei2 , Zahra Bahari3 , Gholamhossein Meftahi 4 |
1- Department of Biology, Azad Islamic University, North Branch, Tehran, Iran
2- Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
3- Department of Physiology and Medical Physic, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
4- Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran , hossein.meftahi@bmsu.ac.ir |
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Abstract: (3981 Views) |
Background and Aim: In this study, we investigated the effect of immobilization stress during pregnancy on the spatial memory, anxiety-like behavior and brain BDNF level in F1 generation male NMRI mice.
Materials and Methods: Twenty female pregnant mice were randomly divided into stress and control groups. The stress group received stress using a restraint cylinder (6 cm ID, 20 cm L) 60 min/day from the 1st to the 15th day of pregnancy. The control group did not receive stress. The male F1 offspring was nursed by their mothers (n=10/group) until reaching weights between 20 and 25 g. Then they were tested for spatial memory using Barnes maze and anxiety-like behavior by the elevated plus-maze. Also, brain BDNF level was measured by means of an ELISA reader.
Results: Barnes maze test results showed that the time and distance to reach the target hole were significantly (p<0.001) increased in the stress group. Also, the number of errors and anxiety-like behavior in the stress group were significantly (p<0.001) increased compared to those in the control group (p<0.001). BDNF level in the brain was significantly (p<0.05) higher in the control group.
Conclusion: The present study indicated that prenatal stress can lead to decreased level of BDNF in the brain of the offspring which may result in spatial memory disorder and anxiety-like behavior.
Key Words: Restraint stress, Barnes maze, Spatial memory, Anxiety-like behavior, BDNF.
Received: Feb 17, 2018 Accepted: May 22, 2018 |
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| Keywords: Restraint stress, Barnes maze, Spatial memory, Anxiety-like behavior, BDNF. |
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Full-Text [PDF 319 kb]
(1725 Downloads)
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Type of Study: Original Research |
Subject:
General
Received: 2018/07/31 | Accepted: 2018/07/31 | Published: 2018/07/31
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