:: Volume 23, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2018) ::
SJKU 2018, 23(2): 57-69 Back to browse issues page
Evalution of phytochemical and anticancer properties of cotton thistle (Onopordon leptolepis DC.) extract on the survival of CACO2 cancer cells
Neda Mirzaei 1, Babak Mokhtari Dr2, Maryam Kolahi Dr 3
1- Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Iran.
2- Associated Professor Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Iran.
3- Assistant Professor Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Iran , m.kolahi@scu.ac.ir
Abstract:   (462 Views)
Background and Aim: The aim of this study was to detect the organic compounds of Onopordon leptolepis DC. and also evaluate the anticancer and anti-oxidant effects of the plant extract on CaCO2 cells.
Materials and Methods: The effective compounds of air branches of Onopordon leptolepis DC. were studied using phytochemicals methods. Using GC-MS device the components of  ethanolic extract of air branches of Onopordon leptolepis DC. were isolated by Soxhlet method. Also the ethanolic extract of three parts of the plant including air branches, leaf, and stem were evaluated for anti-cancer properties using MTT and NBT methods based on a complete randomized block design.
Results: Using GC-MS device we identified 27 components in the extract. Among them, A-Neooleana-3 (5), 12-diene (78.92%), 2 (1H) Naphthalenone, 3.5,6,7,8,8a-hexahydro-4, 8a-dimethyl-6 - (1-methylethenyl) - (13.65%), and hexadecanoic acid (1.33%), were the main components of the extract respectively. In this study, presence of alkaloids, cyanogenic glycosides, tannins, and steroids were confirmed in the plant. The results of cell viability analysis using MTT and free radical activity showed that ethanolic extracts of air branches, leaf, and stem of the plant had anticancer activity. The highest rate of neutralization of free radicals produced by cancer cells was observed in the extract of the air branches of the plant.
Conclusion: The medicinal and anti-cancer properties of this plant may be due to the presence of some of the alkaloids, glycosides, phenols and other phytochemical compounds in the plant which can be involved in the antioxidant activity of the plant. The detected chemical compounds in the plant can be used in the food industry and production of effective chemical medicines for the treatment of cancer.
Key words: Anticancer, Chemical compound, Gas chromatography, Onopordon leptolepis DC.
 
Received: Aug 14, 2017     Accepted: Apr 20, 2018 
Keywords: Anticancer, Chemical compound, Gas chromatography, Onopordon leptolepis DC.
Full-Text [PDF 719 kb]   (223 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/05/31 | Accepted: 2018/05/31 | Published: 2018/05/31
References
1. 1. Valizadeh E, Fatholahy Zonouz N, Zand A, Shahbazi S, Malekian A. Evaluation of antioxidant potentials of extracts of cotton thistle (Onopordum leptolepis DC.) obtained by various solvents. Aust J Crop Sci 2011; 5: 1163-6.
2. Singh RP, Pandey VB. Nivetin, a neoflavonoid from echinops niveus. Phytochemistry 1999; 29: 680-1. [DOI:10.1016/0031-9422(90)85148-9]
3. Briese DT. Impact of the Onopordum capitulum weevil Larinus latus on seed production by its host-plant. J Appl Ecol 2000; 37: 238-46. [DOI:10.1046/j.1365-2664.2000.00489.x]
4. Pourhoseingholi M A, Fazeli Z, Fazeli-Bavandpour F S, Abadi A. Study of mortality trends of colorectal cancer in Iran between 1995 and 2004. Medical Sciences 2014; 23: 16-20. [In Persian]
5. Kolahi M, Mokhtari B, Mirzaee N. A phytochemical study and comparison of the effect of pomegranate extracts spongy tissue on colon cancer cells caco2. Jundishapur Sci Med J 2016; 15: 201-15. [In Persian]
6. Mahdavi Meyman Z, Mir-Tajaddinni SM. Phytochemical evaluation of 30 plant species collected from Shahrbabak (Kerman/Iran). JKMU 2006; 13: 95-102. [In Persian]
7. Kokate CK, Gokhale SB, Purohit APA. Textbook of pharmacognosy. 29nd ed. Maharashtra: Nirali Prakashan, 2008: 635.
8. Arora M, Kaur P. Phytochemical screening of orange peel and pulp. IJRET 2013; 12: 517-22.
9. Satheesh Kumar B, Suchetha Kumari N, Vadisha SB, Sharmila KP, Mahesh Prasad B. Preliminary phytochemical screening of various extracts of Punica granatum peel, whole fruit and seeds. NUJHS 2012; 2: 34-8.
10. Nasrabadi M, Halimi M, Nadaf M. Phytochemical screeningand chemical composition of extract of Muscari neglectum. Middle-East J Sci Res 2013; 14: 566-9.
11. Mokhtari B, Kolahi M, Mirzaei N. A comparison study of extraction methods and mass spectrum for compounds in Echinops dichrous and comparison of effects of extracts on colon cancer cells CaCo2. JMP 2017; 2: 145-57. [In Persian]
12. Zeping H, Xiaoxia Y, Ho PCL, Chan SY, Heng PWS, Eli C, et al. Herb-drug interactions: a literature review. Drugs 2005; 65: 1239-82. [DOI:10.2165/00003495-200565090-00005]
13. Khazraei-Moradian S, Andalib A, Ganjalikhani-Hakemi M, Safari Z, Zare A, Kardar GhA. The effect of protein extract of Licorice root in proliferation of HT-29 and CT26 cancer cell lines. J Isfahan Med Sch 2014; 32: 1338-46.
14. Freeman R, King B.A. Modification to the N.B.T. Test. Lancet 1971; 2: 1154. [DOI:10.1016/S0140-6736(71)91312-2]
15. Adams RP. Identification of essential oil components by gas chromography/mass spectrometry. Allured publishing corporation. 4nd ed. United States: Allured Publishing Corporation, 2007: 804.
16. Glasby JS. Dictionary of plants containing secondary metabolites. UK: Taylor and Francis, Basingstoke, 1991: 100-200.
17. Knapp DR. Handbook of Analytical Derivatization reactions. United States: John Wiley & Sons Inc, 1979: 18.
18. Davarynejad G, Taghizadeh S, Asili J. Effect of different solvents on total phenolic contents and antioxidant activity of Zizyphus jujube miller fruits. Journal of Horticultural Science 2017; 31: 158-66.
19. Yao H, Qing L. Antioxidant activities of barley seeds extracts. Food Chem 2007; 102: 732-7. [DOI:10.1016/j.foodchem.2006.06.051]
20. Masoko D, Galolo SS, Mokgotho MP, Eloff JN, Howard Rl, Mampara LJ. Evaluation of the antioxidant, antibacterial andante prolife rative activities of the aceton extract of the roots of Sen naitulical (fabaceue). Afr J Tradit Complement Altern Med 2010; 7: 138-48. [DOI:10.4314/ajtcam.v7i2.50873]
21. Zarezadeh Mehrizi RA, Emam-Djomeh Z, Shahedi Bagh Khandan M, Loni E Akhavan HR, Biabani J. Identification and quantification of anthocyanins in pomegranate peel extract. JFST 2015; 12: 31-40.
22. Zolfaghari B, Yegdaneh A. Recent advances in extraction methods of medicinal plant components. J Herbal Drugs 2010: 1: 51-5. [In Persian]
23. Mohamadi M, ElhamiRad AH, Pourfallah Z. Determination of total phenolic compound contents and antioxidant capacity of persimmon skin. Journal of Food Hygiene 2012; 2: 41-55.
24. Locatelli M, Travaglia F, Coisson J.D, Martelli A, Stevigny C, ArlorioM. Analytical Methods Total Antioxidant Activity of Hazelnut Skin (Nocciola Piemonte PGI): Impact of different roasting conditions. Food Chem 2010; 119: 1647-55. [DOI:10.1016/j.foodchem.2009.08.048]



XML   Persian Abstract   Print



Volume 23, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2018) Back to browse issues page