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Simulated microgravity effects on cell cycle in adipose derived stem cells
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Farid Ebnerasuly1   , Zahra Hajebrahimi 2, Seyed Mehdi Tabaie3 , Mojtaba Darbouy4 |
1- Department of Biology, Fars Science and Research Branch , Islamic Azad University, Marvdasht, Iran.
2- Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, Iran , hajebrahimi@ari.ac.ir
3- Medical Laser Research Center, Iranian Academic Center for Education, Culture and Research (ACECR), Tehran, Iran.
4- Department of Biology, Fars Science and Research Branch, Islamic Azad University, Marvdasht, Iran. |
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Abstract: (4140 Views) |
Background and Aim: In recent years, biophysical forces especially microgravity increasingly has been used for cell manipulation, in particular cell differentiation and tissue engineering. Microgravity, as a mechanical factor, has been demonstrated to stimulate cell proliferation and differentiation. In this study, we investigated the impact of simulated microgravity condition generated by one-axis clinostat, on cell cycle and apoptosis rate of adipose derived stem cells (ADSCs).
Material and Methods: After isolation and characterization of human ADSCs, they were exposed to simulated microgravity condition generated by one-axis clinostat, for 3 days. After 3 days of clinorotation, control and microgravity samples were stained with PI, then, we evaluated cell cycle and apoptosis rate.
Results: Isolated cells expressed mesenchymal markers and were able to differentiate into adipocytes and osteocytes. Our results showed that 3 days of simulated microgravity induced by one-axis clinostat had no statistically significant effects on the cell cycle and apoptosis rate.
Conclusion: Previous studies have shown that simulated microgravity induced by 3-axis clinostat or RPM altered cell cycle and increased apoptosis rate. Based on our data, it seems that shear stress generated by one-axis clinostat is much less than that generated by three-axis clinostat. Therefore, we recommend use of one-axis clinostat for evaluation of cell differentiation and cell therapy under microgravity conditions for the future studies.
Keywords: Adipose derived stem cells, Simulated microgravity, One-axis clinostat.
Received: Feb 4, 2017 Accepted: Jul 8, 2017 |
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| Keywords: Adipose derived stem cells, Simulated microgravity, One-axis clinostat. |
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Full-Text [PDF 273 kb]
(1482 Downloads)
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Type of Study: Original Research |
Subject:
General
Received: 2017/11/20 | Accepted: 2017/11/20 | Published: 2017/11/20
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